Abstract: Objective]With different resistant island cottons as material,using real-time fluorescence quantitative PCR technology to analyze the expression levels of 1 1 genes related to wilt resistance in cotton, this project aims to provide candidate gene resources for breeding resistance varieties to Fusarium wilt of sea island cotton and its mechanism.[Method]Sea Island cotton resistance to Fusarium wilt disease 06-146,the susceptible materials of Xinhai 14 and the parents of the hybrid RIL high generation materials,10893 (super resistant),10895 (supper resistant),10879 (sensible)and 10796 (susceptible)were chosen as the experi-mental materials and at the same time,inoculation treatment was carried out.The seedlings of 0 h,4 h,10 h, 18 h,h,28,40 h,respectively,were taken to extract the mRNA and went through reverse transcription. Based on the known EST sequences of Fusarium wilt,resistance in upland cotton and the resistance related gene sequences screened from the Sea Island cotton disease resistance gene sequences,the real-time fluores-cence quantitative PCR reaction was carried out.According to the expression of different genes in different ma-terials,the relationship between different genes and the resistance to Fusarium wilt of cotton were analyzed.[Result]Four genes,CFW3,CFW10,comp62810,comp71372 in cotton may play a more important role in the process of fighting against Fusarium wilt of Sea Island cotton.In particular,the comp62810 and comp71372 gene,which were obtained from the transcriptional group of the disease resistant material and the susceptible material,were related to the disease resistance.[Conclusion]Through the analysis,it is conclu-ded that the sensitivity of different cotton varieties to the infection of the pathogen is different,which can be applied to determine the roles of the genes.

摘要： 【目的】以不同抗性海岛棉为材料，利用实时荧光定量PCR技术对11个棉花抗枯萎病相关基因进行表达量分析，为海岛棉抗枯萎病育种及抗病机理提供候选基因资源。【方法】选择海岛棉抗枯萎病材料06－146、易感材料新海14号，及以其为父母本杂交的RIL系高代材料10893（超抗）、10895（高抗）、10897（感病）、10796（易感）为实验材料，进行接菌处理。分别取接菌0、4、10、18、28和40 h后的幼苗下胚轴，提取mRNA并反转录。根据已知陆地棉抗枯萎病相关的EST序列和海岛棉抗病转录组测序筛选到的抗病相关基因序列设计引物，进行实时荧光定量 PCR反应。根据各基因在不同材料中的表达值，分析各基因与海岛棉抗枯萎病间的关系。【结果】CFW3、CFW10、comp62810、comp71372四个基因在海岛棉抗枯萎病过程中可能起着比较重要的作用，尤其是利用抗病材料和感病材料转录组测序后得到的与抗病性有关的comp62810、comp71372基因。【结论】不同棉花品种对病菌侵染的灵敏程度，不同的品种的灵敏度不同，用以判断基因的作用。