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Effects of NaCl Stress on Seed Germination of Six Lycium ruthenicum Murr.Types

LIU Qiu-chen;FENG Jian-rong;HAO Yu-jie;FAN Xin-min;ZHANG Yong-dong   

  • Received:2016-11-25 Revised:2016-11-25 Online:2016-11-25 Published:2016-11-25

NaCl胁迫对六个不同类型黑果枸杞种子萌发的影响

刘秋辰;冯建荣;郝玉杰;樊新民;张永东   

  1. 石河子大学农学院/特色果蔬栽培生理与种质资源利用兵团重点实验室,新疆石河子,832000;新疆果王生态农业科技开发有限公司,乌鲁木齐,830001

Abstract: Objective]The objective of this experiment is to compare the salt tolerance of six types of Lycium ruthenicum Murr.The results of this study might provide a technical basis for promoting the best type in Northern Xinjiang.[Method]The study included three ecotypes of L.ruthenicum (Xining wild type,Korla wild type,Wuwei wild type)and the corresponding promising selection (Xining promising selection,Korla promising selection,Wuwei promising selection).The seeds were treated with NaCl concentrations of 50, 100,150,200,250,and 300 mmol/L.A standard of 50 percent germination rates was set for production re-quirements.Seeds were considered to be under significant duress when the germination rates were less than 50 percent.Seed germination rate,relative salt injury rate,salt tolerance concentration,and seedling growth were measured under different NaCl concentrations.[Result]Germination rate,seedling growth decreased as NaCl concentrations increased between 50 and 300 mmol/L.Regression analysis was conducted to determine the relationship between NaCl concentration and germination rate.The regression analysis indicated that the critical NaCl concentration was greater for the promising selection type seeds than for wild type seeds.The crit-ical NaCl concentration was 150-300 mmol/L for the promising selection type seeds and 100-150 mmol/L for the wild type seeds.[Conclusion]The results indicated that among the six types,the promising selection Wuwei type and the promising selection Korla type had relatively high salt tolerance,whereas wild Xining type and wild Wuwei type had relatively low salt tolerance.

摘要: 【目的】对6个不同类型的黑果枸杞种子耐盐性进行评价比较,筛选出耐盐性较高的类型,为在新疆北疆地区推广提供理论基础。【方法】以西宁野生、库尔勒野生、武威野生三个生态型及其相应的优选系:西宁优系、库尔勒优系、武威优系黑果枸杞种子为试验材料,在50、100、150、200、250、300 mmol/L NaCl浓度下进行种子发芽试验,以发芽率达到50;为生产要求,低于50;为受到明显胁迫的标准,测定不同浓度NaCl对发芽率、相对盐害率、耐盐浓度及幼苗生长的影响。【结果】在50~300 mmol/L NaCl浓度处理下,发芽率、胚根、胚轴长随NaCl浓度升高总体呈显著下降趋势;用种子发芽率和NaCl浓度进行回归分析,发现优选型黑果枸杞种子耐盐临界浓度显著高于野生型,优选型耐盐临界浓度为150~300 mmol/L,野生型耐盐临界浓度为100~150 mmol/L。【结论】武威优系、库尔勒优系耐盐能力显著较强;西宁优系、库尔勒野生中等;西宁野生、武威野生显著较差。