Abstract: [Objective] The candidate mutants of maize ZmCIPK23 gene were continuously backcrossed and self-pollinated with B73 in fields, and finally homozygous mutants with B73 background were obtained, which laid the material foundation for studying the function of this gene.[Method]The candidate mutants of ZmCIPK23 were screened from a maize Mutant library.They were planted and analyzed by nested PCR.The positive plants with Mu-insertion in the ZmCIPK23 gene were self-pollinated and backcrossed with B73 in the field.Thus the homozygous mutants were obtained.[Result]The homozygous mutant of ZmCIPK23 gene was obtained by PCR.[Conclusion]The homozygous mutant (zmcipk23) of Mu insertion of ZmCIPK23 gene was obtained.This study has provided a foundation for the functional analysis of the ZmCIPK23 gene.

摘要： [目的]玉米ZmCIPK23基因的候选突变体连续与玉米自交系B73回交并自交,最终得到以B73为背景的纯合突变体,为研究该基因的功能奠定材料基础.[方法]从玉米Mutator(Mu)突变体库中定向筛选到玉米ZmCIPK23基因的候选突变体.在田间种植候选突变体,利用巢式PCR对这些后代植株进行鉴定,选择阳性植株与B73杂交.通过连续的回交及自交,最终获得该基因的纯合突变体.[结果]通过PCR鉴定,获得了以B73为背景的ZmCIPK23基因纯合的突变体zmcipk23.[结论]获得了玉米ZmCIPK23基因的Mu插入的纯合突变体zmcipk23,为研究该基因的功能奠定了基础.