]*>","")" /> A half-sterilized method acquiring sterilized stem- segments with single bud of grape
Xinjiang Agricultural Sciences

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A half-sterilized method acquiring sterilized stem- segments with single bud of grape

YI Li-juan;ZENG You-ling;HE Bin;ZHANG Fu-chun   

  • Received:2005-06-25 Revised:2005-06-25 Online:2005-06-25 Published:2005-06-25

半无菌表面灭菌获得葡萄单芽茎段无菌材料的方法

易丽娟;曾幼玲;贺宾;张富春   

  1. 新疆生物资源基因工程重点实验室,新疆大学生命科学与技术学院,新疆,乌鲁木齐,830046;新疆农科院经作所,新疆,乌鲁木齐,830000





摘要： 介绍了对当年生葡萄嫩枝利用半无菌灭菌方法结合75;酒精处理3～5 s,无菌水洗一次,0.1;升汞3 min,进行表面灭菌,无菌处理效果好,污染率低,且单芽萌发情况好.