Abstract: [Objective] The explants of two inbred oil sunflower (1538-3 and 1573-2) were used to establish the tissue regeneration culture system, and the optimum strain was selected to lay the foundation for genetic transformation.[Method]Two different inbred lines of oil sunflower were taken respectively, and the regeneration of adventitious bud of oil sunflower was induced from two aspects of indirect and direct organs by different hormone ratio.[Result]The results showed that inbred lines 1538-3 was easy to form callus in the mediums containing NAA, 6-Benzylaminopurine (6-BA) and kinetin (KT), but all the callus were white with high water damage level.The sunflower cotyledonary node could induce adventitious buds when the concentration of 6-BA was 0 to 1.0 mg/L, which couldn't be observed in other hormone combinations.The combination of MS + 0.6 mg/L 6-BA + 0.1 mg/L GA3 was most suitable for the induction of adventitious bud from inbred lines 1573-2 hypocotyls explants.While for inbred line 1538-3, the most suitable medium was MS + 0.5 mg/L 6-BA + 0.05 mg/L GA3.The best concentration of NAA for inducing the root of adventitious buds ranged from 0 to 0.2 mg/L in both inbred lines.[Conclusion]The ability of the two genotypes of oil sunflower to induce adventitious bud is different.Among them, the inbred line 1538-3 has stronger regeneration ability, and it is better to choose the 1538-3 axis as the transformation receptor in the genetic transformation.

摘要： [目的]取两种自交系油葵(1538-3和1573-2)的不同外植体,建立组织再生培养体系,选出最适品系,为遗传转化奠定基础.[方法]分别取两种自交系油葵的不同组织,采用不同激素配比,从器官间接和直接发生两个方面分别诱导油葵不定芽再生.[结果]以油葵自交系1538-3的不同外植体为材料,在含有1-萘乙酸(NAA)、6-苄氨基嘌呤(6-BA)、激动素(KT)的培养基中都易形成愈伤组织,但均表现为白色、水渍化程度高,无分化能力;6-BA浓度在0~1.0 mg/L时,子叶节可诱导出不定芽,其他激素配比中未见此现象;以胚轴为外植体时,油葵1573-2自交系不定芽诱导的最佳培养基为MS + 0.6 mg/L 6-BA + 0.1 mg/L GA3;1538-3自交系不定芽诱导的最佳培养基为MS + 0.5 mg/L 6-BA + 0.05 mg/L GA3;两种自交系诱导不定芽生根时NAA的最佳浓度为0~0.2 mg/L.[结论]两种基因型油葵诱导不定芽的能力差别较大,其中自交系1538-3再生能力较强,遗传转化时选用1538-3的胚轴作为转化受体时较好.