Construction of Fingerprinting Map and Purity Identification of Main Cotton Cultivars in Xinjiang

doi:10.6048/j.issn.1001-4330.2019.02.002

Abstract

Abstract: 【Objective】 This project aims to construct fingerprinting map and analyze the purity of four main cotton cultivars in Xinjiang in the hope of providing a basis for molecular identification of cotton varieties.【Method】Typically representative early-mature cotton varieties in northern Xinjiang were used as materials, 25 pairs of primers with high polymorphism, good stability selected from 1,000 pairs of SSR primers as the main extension backbone varieties to construct fingerprinting map, and 10 primers were selected to analyze the purity of 4 varieties of cotton.【Result】The number of alleles detected was 70 and the number of alleles detected by each marker ranged from 2 to 7, with an average of 2.8; The polymorphism information content ranged from 0.169,0 to 0.872,9, with an average of 0.688,3; 4 specific primers could completely differentiate the 4 cotton varieties from each other and their construct fingerprinting map. 10 pairs of primers were used to detect the purity of 4 varieties, and the results showed that the variety with highest purity was Xi 62, with the purity of 99.5% and the variety with lowest purity was Xinluzao 6, whose purity was 92.0%.【Conclusion】The fingerprint and the identification purity of the four cotton varieties were constructed by the SSR molecular marker technique, and the theoretical basis for the construction and the purity identification of the fingerprint of the subsequent main varieties was established.

Key words: cotton; SSR molecular markers; DNA fingerprinting map; purity

摘要： 【目的】构建新疆主栽骨干棉花品种的指纹图谱,分析棉花品种纯度,为棉花品种提供分子鉴定依据。【方法】以典型代表性的新疆北疆早熟棉花主推骨干棉花品种为材料,利用SSR分子标记技术从1 000多对SSR引物中筛选出稳定性好、多态性高的25对核心引物构建指纹图谱,从核心引物中筛选10对引物作为标记,分析4个棉花品种的纯度。【结果】检测到等位基因数70个,每个标记检测到的等位基因数介于2~7个,平均2.8个;引物多态信息量(PIC)值介于0.169 0~0.872 9,平均值为0.688 3;利用4个特征引物将4个棉花品种一次性完全区分开,构建供试品种的指纹图谱;利用10对引物检测4个品种的纯度,新陆早6号纯度变化范围85%~100%,系62纯度变化范围95%~100%。【结论】构建了4个棉花品种的指纹图谱及鉴定纯度,系62号纯度最高,为99.5%,新陆早6号纯度最低,为92.0%。

关键词: 棉花, SSR分子标记, DNA指纹图谱, 纯度

CLC Number:

S562
S188

Buka Ouerna, ZHAG Wen, ZEAG Qing-tao, MA Li-juan, LU Tao, CAI XIAO-LI, ZHAO FU-qiang,. Construction of Fingerprinting Map and Purity Identification of Main Cotton Cultivars in Xinjiang[J]. Xinjiang Agricultural Sciences, 2019, 56(2): 207-215.

布卡·欧尔娜, 张文, 曾庆涛, 马丽娟, 逯涛, 蔡晓莉, 赵富强. 新疆主栽骨干棉花品种指纹图谱构建及纯度鉴定[J]. 新疆农业科学, 2019, 56(2): 207-215.

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