Rapid Identification of Farmland's Cicadellidae in Southern Foothills of Pamirs Plateau in China Based on DNA Bar-coding Genes

doi:10.6048/j.issn.1001-4330.2020.12.016

Xinjiang Agricultural Sciences ›› 2020, Vol. 57 ›› Issue (12): 2299-2309.DOI: 10.6048/j.issn.1001-4330.2020.12.016

• Horticultural Special Local Products·Storage and Preservation Processing·Plant Protection • Previous Articles Next Articles

Rapid Identification of Farmland's Cicadellidae in Southern Foothills of Pamirs Plateau in China Based on DNA Bar-coding Genes

ZHANG Haiyan^1,2, CHEN Guanghui³, ZHANG Xiuying^1,2, WANG Yutao^1,2

1. College of Life and Geographic Sciences, Kashi University, Kashi Xinjiang 844000, China;
2. Key Laboratory of Biological Resources and Ecology of Pamirs Plateau in Xinjiang Uygur Autonomous Region, Kashi Xinjiang 844000, China;
3. Technology Center of Kashi Customs, Kashi Xinjiang 844000, China

Received:2019-04-02 Online:2020-12-20 Published:2020-12-24
Correspondence author: WANG Yutao (1979 -), male, Zhenyuan, Gansu Province, Professor, Doctor, Research direction: Animal ecology and molecular evolution, (E-mail) wangytgs@163.com
Supported by:
Supported by the University Scientific R& D Project of Xinjiang Uygur Autonomous Region (XJEDU20201017) and "Light of the West" Project of Chinese Academy of Sciences (2018-XBQNXZ-A1-002)

基于DNA条形码基因快速鉴定帕米尔高原南麓部分地区农田叶蝉

张海燕^1,2, 陈光辉³, 张秀英^1,2, 王玉涛^1,2

1.喀什大学生命与地理科学学院,新疆喀什,844000;
2.新疆帕米尔高原生物资源与生态重点实验室,新疆喀什,844000;
3.喀什海关技术中心,新疆喀什,844000

通讯作者: 王玉涛(1979-),男,甘肃镇原人,教授,博士,研究方向为动物生态与分子进化,(E-mail)wangytgs@163.com
作者简介:张海燕(1986-),女,甘肃武威人,硕士研究生,研究方向为有害生物防治工程,(E-mail)865225679@qq.com
基金资助:
新疆维吾尔自治区高校科研计划(XJEDU20201017);中国科学院“西部之光”项目(2018-XBQNXZ-A1-002)

Abstract

Abstract: 【Objective】 More convenient and efficient identification techniques need to be explored to improve the identification rate.which can be used for rapid and accurate identification of Cicadellidae and provide basic data for the identification and prevention of Cicadellidae in this area. 【Methods】 In this research DNA bar-coding technology was used to obtain DNA bar-code of Cicadellidae, during which COI, Cytb, ITS2 genes were selected, PCR amplification was applied with universal primers and sequenced directly. Bioinformatics analysis software was used to compare and analyze the similarity of target gene sequences. Phylogenetic tree was constructed to analyze genetic and evolutionary relationship. 【Results】 19 mtDNA sequences of COI,Cytb and ITS2 genes were acquired as DNA barcodes of Cicadellidae, including six COI genes, seven Cytb genes and 6 Cytb genes; Among them, one was a new COI sequence, five Cytb sequences and 6 ITS2 sequences, all of which were the first to be reported in this region. 【Conclusion】 Nineteen DNA bar codes of Cicadellidae are obtained, the rapid and accurate identification of 4 species of farmland’s Cicadellidae in the southern foothills of Pamirs Plateau came true.

Key words: Cicadellidae; mtDNA; DNA barcode; identification

摘要： 【目的】 研究更加便捷高效的鉴定技术,提高叶蝉鉴定的速率,为帕米尔高原南麓部分地区叶蝉的鉴定及防治提供基础数据。【方法】 利用DNA条形码技术,选择mtDNA COI、Cytb、ITS2基因,运用通用引物PCR扩增并直接测序,借助生物信息学分析软件对比分析目的基因序列的相似性,构建系统进化树,分析遗传进化关系,获取叶蝉DNA条形码。【结果】 获得了19条mtDNACO1、Cytb及ITS2基因序列,COI基因6条、Cytb基因7条、ITS2基因6条,可作为叶蝉的DNA条形码,包括其中1条COI新序列,5条Cytb和6条ITS2序列。【结论】 获得19条叶蝉的DNA条形码,实现了帕米尔高原南麓部分地区农田4种叶蝉的快速准确鉴定。

关键词: 叶蝉科, mtDNA, DNA条形码, 鉴定

CLC Number:

S188
S43

ZHANG Haiyan, CHEN Guanghui, ZHANG Xiuying, WANG Yutao. Rapid Identification of Farmland's Cicadellidae in Southern Foothills of Pamirs Plateau in China Based on DNA Bar-coding Genes[J]. Xinjiang Agricultural Sciences, 2020, 57(12): 2299-2309.

张海燕, 陈光辉, 张秀英, 王玉涛. 基于DNA条形码基因快速鉴定帕米尔高原南麓部分地区农田叶蝉[J]. 新疆农业科学, 2020, 57(12): 2299-2309.

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Rapid Identification of Farmland's Cicadellidae in Southern Foothills of Pamirs Plateau in China Based on DNA Bar-coding Genes

基于DNA条形码基因快速鉴定帕米尔高原南麓部分地区农田叶蝉

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