Insecticide Resistance Monitoring and Target Molecular Detection of Diamide Insecticides in Field Populations of Ostriniafurnacalis

doi:10.6048/j.issn.1001-4330.2021.04.012

Xinjiang Agricultural Sciences ›› 2021, Vol. 58 ›› Issue (4): 690-699.DOI: 10.6048/j.issn.1001-4330.2021.04.012

• Soil Fertilizer·Plant Protection·Microbes • Previous Articles Next Articles

Insecticide Resistance Monitoring and Target Molecular Detection of Diamide Insecticides in Field Populations of Ostriniafurnacalis

ZHI Haoyu¹, WANG Zhihui², WANG Xingzai¹, DING Xinhua³, JIA Zunzun³, GUO Wenchao², JIANG Weihua¹

1. College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China;
2. Xinjiang Special Environmental Microbiology Laboratory/Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China;
3. Key Laboratory of Integrated Crop Pest Management in Northwestern Oasis of China,MOARA,P. R. China, Institute of Plant Protection,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China

Received:2020-12-01 Online:2021-04-20 Published:2021-04-25
Correspondence author: JIANG Weihua(1969-), female, native place: Nantong, Jiangsu province, associate professor, master supervisor, research interest: molecular biology and physiological toxicology of insects,(E-mail)jwh@njau.edu.cn
GUO Wenchao(1966-), male, native place: Gucheng, Hebei province, doctoral supervisor. research interest: integrated control of agricultural insects, (E-mail)gwc1966@163.com
Supported by:
Project of National Key R & D Program of China (2017YFD0201800)

亚洲玉米螟的抗药性监测及双酰胺类药剂抗性靶标分子检测

支昊宇¹, 王智慧², 王兴仔¹, 丁新华³, 贾尊尊³, 郭文超², 姜卫华¹

1.南京农业大学植物保护学院,南京 210095;
2.新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐 830091;
3.新疆农业科学院植物保护研究所/农业部西北荒漠绿洲作物有害生物综合治理重点实验室,乌鲁木齐 830091

通讯作者: 姜卫华(1969-),女,江苏人,副教授,硕士生导师,研究方向为昆虫分子生物学及生理毒理学,(E-mail)jwh@ njau.edu.cn
郭文超(1966-),男,河北人,研究员,博士生导师,研究方向为农业害虫综合防治,(E-mail)gwc1966@163.com
作者简介:支昊宇(1994-),男,硕士研究生,研究方向为昆虫生理生化与分子生物学,(E-mail)1415031496@qq.com
基金资助:
国家重点研发计划(2017YFD0201800)

Abstract

Abstract: 【Objective】 The study aims to provide a basis for guiding the rational use of insecticides and resistance management for efficient controlling Ostriniafurnacalis through resistance monitoring and detection of field populations to the commonly used insecticides mainly including diamides, acting on the ryanodine receptor (RyR). 【Methods】 In this study, it was monitored for the resistance to insecticides including chlorantraniliprole, cyantraniliprole, aricaandlangat against O.furnacalis from Urumqi (UQ), Shule (SL1,), Yining (YN1) and Zepu (ZP) in 2018 and Urumqi (UQ), Shule(SL2), Yining (YN2) and Changji (CJ) in 2019 in Xinjiang using artificial diet dipping method. Meanwhile, we analyzed and detected expression changes and mutation of ryanodine receptor genes in the populations collected in 2018 and 2019 by quantitative real-time PCR (qPCR) and PCR amplification, respectively. 【Results】 SL1 population had reduced sensitivity to chlorantraniliprole and medium level of resistance to arica with resistance ratios (RR) of 3.67 and 20.17-fold compared to a relatively sensitive population, respectively, YN1 population developed moderate resistance to cyantraniliprole and arica with RR being 15.26 and 14.10-fold respectively, and ZP population had a moderate level of resistance to arica and langat with RR of 10.97 and 13.48-fold, respectively. Among populations collected in 2019, SL2 population showed reduced sensitivity and the rest populations, namely UQ, YN2 and CJ, exhibited low to extremely high level resistance to cyantraniliprole with RR of 3.61, 8.14, 96.49 and 1,429-fold compared with the susceptible population JZ-S, respectively. The qPCR results showed that there was no significant difference in the expression of ryanodine receptor genes among the different field populations collected in 2018, while those in CJ and YN2 populations collected in 2019 increased significantly by 4.04 and 3.86 times compared with the susceptible strain. The gene mutation test results showed that it was detected for a mutation in the ryanodine receptor of CJ population, in which glutamic acid (E) was replaced by aspartic acid (D) at position 1,424 (E1424D) with 30% of the mutation frequency.【Conclusion】 The resistance of the inter-field population of O. furnacalis in Xinjiang to diamides varies from low to medium levels, and its resistance was related to the increase of the expression and mutation of the ryanodine receptor gene.

Key words: Ostriniafurnacalis; chlorantraniliprole; cyantraniliprole; resistance; ryanodine receptor; gene expression; point mutation

摘要： 【目的】 研究监测和检测亚洲玉米螟田间种群对双酰胺类等药剂的抗性,为指导田间合理用药及抗性治理提供依据。【方法】 于2018和2019年,采用人工饲料浸药法,测定新疆乌鲁木齐市、伊宁市、疏勒县、泽普县和昌吉市亚洲玉米螟田间种群对氯虫苯甲酰胺、溴氰虫酰胺、阿立卡(噻虫嗪·高效氯氟氰菊酯)和亮泰(阿维菌素·氯虫苯甲酰胺)的抗性水平。利用定量PCR分析不同田间种群鱼尼丁受体(ryanodine receptor,RyR)基因表达量的变化及利用PCR扩增检测RyR分子突变。【结果】 2018年的测试种群中,与相对种群相比,疏勒种群对氯虫苯甲酰胺为敏感性下降,对阿立卡达到中等水平抗性,抗性倍数(RR)分别为3.67和20.17倍;伊宁种群分别对溴氰虫酰胺、阿立卡产生了15.26和14.10倍的中等水平抗性;泽普种群对阿立卡和亮泰具有中等水平抗性,RR分别为10.97和13.48倍。2019采集的田间种群中,与敏感种群JZ-S相比,除了疏勒种群对溴氰虫酰胺为敏感性降低,其它3个种群(乌鲁木齐市、伊宁市和昌吉市)都产生了低至极高水平的抗性,RR分别为3.61、8.14、96.49和1 429倍。2018年新疆亚洲玉米螟各种群的RyR基因表达量无显著差异,而2019年昌吉、伊宁种群的RyR基因表达量显著升高(4.04、3.86倍);昌吉种群RyR第1 424位谷氨酸(E)替换为天冬氨酸的突变(E1424D),突变频率为30%。【结论】 新疆亚洲玉米螟田间种群对双酰胺类杀虫剂产生低至中等水平抗性,其抗性与鱼尼丁受体基因的表达量增加和突变有关。

关键词: 亚洲玉米螟, 氯虫苯甲酰胺, 溴氰虫酰胺, 抗药性, 鱼尼丁受体, 基因表达, 突变

CLC Number:

S435.132

ZHI Haoyu, WANG Zhihui, WANG Xingzai, DING Xinhua, JIA Zunzun, GUO Wenchao, JIANG Weihua. Insecticide Resistance Monitoring and Target Molecular Detection of Diamide Insecticides in Field Populations of Ostriniafurnacalis[J]. Xinjiang Agricultural Sciences, 2021, 58(4): 690-699.

支昊宇, 王智慧, 王兴仔, 丁新华, 贾尊尊, 郭文超, 姜卫华. 亚洲玉米螟的抗药性监测及双酰胺类药剂抗性靶标分子检测[J]. 新疆农业科学, 2021, 58(4): 690-699.

References 27

[1]	王振营,王晓鸣. 我国玉米病虫害发生现状、趋势与防控对策[J].植物保护,2019,45(1): 1-11.WANG Zhenying, WANG Xiaoming. Current status and management strategies for corn pests and diseases in China [J]. Plant Protection, 2019,45(1): 1-11.
[2]	丁新华, 刘芳慧, 解玉梅,等. 新疆荒漠绿洲生态区玉米螟田间药效研究及经济效益评价[J]. 新疆农业科学, 2016, 53(4):663-672.DING Xinhua, LIU Fanghui, XIE Yumei, et al. Study on the efficacy and economic benefit of Ostriniafurnacalis in Xinjiang desert oasis ecological region [J]. Xinjiang Agricultural Sciences, 2016, 53(4): 663-672.
[3]	慕立义, 王开运. 亚洲玉米螟对六六六抗药性及取代药剂的研究[J]. 植物保护学报, 1987,14(8):209-215.MU Liyi, WANG Kaiyun. Studies on the resistance of Asian corn borer to BHC and alternation insecticide in China [J]. Journal of Plant Protection, 1987, 14(8): 209-215.
[4]	慕立义, 王开运. 杀虫剂室内选育亚洲玉米螟抗药性及交互抗性的研究[J]. 植物保护学报,1988, 15(3):209-214.MU Liyi, WANG Kaiyun. Study on effect of resistance Asian corn borer with insecticide in laboratory [J]. Journal of Plant Protection, 1988, 15(3): 209-214.
[5]	魏岑, 魏瑞云, 范贤林,等.玉米螟对E-605抗药性的测定[J]. 植物保护,1987,13(6):28-29.WEI Cen, WEI Ruiyun, FAN Xianlin, et al. Resistance assay of Asian corn borer to E-605 [J]. Journal of Plant Protection, 1987, 13(6):28-29.
[6]	张贵锋, 姜策, 吕广辉,等. 4种杀虫剂对一代玉米螟的田间药效试验[J]. 辽宁农业科学, 2013,(3):73-74.ZHANG Guifeng, JIANG Ce, LÜ Guanghui, et al. Field efficacy test of four insecticides on first generation Ostriniafurnacalis [J]. Liaoning Agricultural Science, 2013,(3): 73-74.
[7]	Lai T C, Li J, Su J Y. Monitoring of beet armyworm Spodoptera exigua (Lepidoptera: Noctuidae) resistance to chlorantraniliprole in China [J]. Pesticide Biochemistry and Physiology, 2011, 101(3):198-205.
[8]	Wang R, Wang J D, Che W N, et al. First report of field resistance to cyantraniliprole, a new anthranilic diamide insecticide, on Bemisiatabaci MED in China [J]. Journal of Integrative Agriculture, 2018, 17(1): 158-163.
[9]	Wang X L, Khakame SK, Ye C, et al. Characterisation of field‐evolved resistance to chlorantraniliprole in the diamondback moth, Plutellaxylostella, from China [J]. Pest Management Science, 2013, 69(5):661-665.
[10]	赵丹丹,周丽琪,张帅,等. 二化螟对双酰胺类杀虫剂的抗药性监测和交互抗性研究[J]. 中国水稻科学, 2017, 31(3): 307-314.ZHAO Dandan, ZHOU Liqi, ZHANG Shuai, et al. Resistance Monitoring and Cross-resistance to the Diamides in the Rice Stem Borer, Chilosuppressalis (Lepidoptera: Pyralidae) [J].Chinese Journal of Rice Science, 2017, 31(3): 307-314.
[11]	Troczka B, Zimmer Ct, Elias J, et al. Resistance to diamide insecticides in diamondback moth, Plutellaxylostella (Lepidoptera: Plutellidae) is associated with a mutation in the membrane-spanning domain of the ryanodine receptor [J]. Insect Biochemistry & Molecular Biology, 2012, 42(11):873-880.
[12]	Guo L, Wang Y, Zhou X G, et al. Functional analysis of a point mutation in the ryanodine receptor of Plutellaxylostella (L.) associated with resistance to chlorantraniliprole [J]. Pest Management Science, 2014, 70(7):1083-1089.
[13]	Guo L, Liang P, Zhou X G, et al. Novel mutations and mutation combinations of ryanodine receptor in a chlorantraniliprole resistant population of Plutellaxylostella (L.)[J]. Scientific Reports, 2014, 4: 6924.
[14]	孙丽娜, 杨代斌, 芮昌辉,等.氯虫苯甲酰胺对小菜蛾鱼尼丁受体基因mRNA表达量的影响[J].农药学学报, 2012, 14(2):136-142.SUN Lina, YANG Daibin, RUI Changhui, et al. Effects of chlorantraniliprole on expression abundance ofryanodine receptor gene mRNA from Plutellaxylostella [J]. Chinese Journal of Pesticide Science, 2012, 14(2):136 -142.
[15]	王少丽, 董钧锋, 李如美, 等. 甜菜夜蛾对氯虫苯甲酰胺抗性种群选育及鱼尼丁受体基因表达特征[J].植物保护学报,2015, 42(3): 425-431.WANG Shaoli, DONG Junfeng, LI Rumei, et al. Selection for resistance in the beet armyworm,Spodoptera exiguato chlorantraniliprole and expression patterns of ryanodine receptor [J]. Journal of Plant Protection, 2015,42(3): 425-431.
[16]	Cui L, Rui C H, Yang D B, et al. De novo transcriptome and expression profile analyses of the Asian corn borer (Ostriniafurnacalis) reveals relevant flubendiamide response genes [J]. BMC Genomics, 2017, 18(1):20.
[17]	沈晋良, 谭建国, 肖斌, 等. 我国棉铃虫对拟除虫菊酯类农药的抗性监测及预报[J]. 昆虫知识, 1991, 28(6):337-341.SHEN Jinliang, TAN Jianguo, XIAO Bin, et al. Prediction and resistance monitoring of Helicoverpaarmigera to pyrethroids in China [J]. KunChongZhiShi, 1991,(6): 337-341.
[18]	Pfaffl M W. A new mathematical model for relative quantification in real-time RT-PCR [J]. Nucleic Acids Research, 2001, 29: e45.
[19]	潘志萍,李敦松.昆虫抗药性监测与检测技术研究进展[J].应用生态学报,2006,17(8):1539-1543.PAN Zhiping, LI Dunsong. Research advance sinmonitoringand detecting insect pesticide-resistance [J]. Chinese Journal of Applied Ecology, 2006, 17(8) :1539-1543.
[20]	Campos M R, Silva T B, Silva W M, et al. Susceptibility of Tutaabsoluta (Lepidoptera: Gelechiidae) Brazilian populations to ryanodine receptor modulators [J]. Pest Management Science, 2015, 71(4): 537-544.
[21]	Sang S, Shu B S, YI X, et al. Cross resistance and baseline susceptibility of Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) to cyantraniliprole in the south of China[J]. Pest Management Science, 2015, 72(5):922-928.
[22]	Zhang S Z, Zhang X L, Shen J, et al. Susceptibility of field populations of the diamondback moth, Plutellaxylostella, to a selection of insecticides in Central China[J]. Pesticide Biochemistry and Physiology, 2016, (132): 38-46.
[23]	SIal A A, Brunner J F. Selection for resistance, reversion towards susceptibility and synergism of chlorantraniliprole and spinetoram in obliquebanded leafroller, Choristoneurarosaceana(Lepidoptera: Tortricidae) [J]. Pest Management Science, 2012, 68(3):462-468.
[24]	邓放,周小毛. 氯虫苯甲酰胺对菜青虫鱼尼丁受体基因表达量的影响[J]. 湖南农业科学, 2014,(7):5-7.DENG Fang, ZHOU Xiaomao. Effects of Chlorantraniliprole on expression level of ryanodine receptor Gene in Pieris rapae [J]. Hunan Agriculture Science, 2014,(7):5-7.
[25]	Jouraku A, Kuwazaki S, Miyamoto K, et al. Ryanodine receptor mutations (G4946E and I4790K) differentially responsible for diamide insecticide resistance in diamondback moth, Plutellaxylostella L [J]. Insect Biochemistry and Molecular Biology, 2020, (118):103308.
[26]	Yao R, Zhao Dd, Zhang S, et al. Monitoring and mechanisms of insecticide resistance in Chilosuppressalis (Lepidoptera: Crambidae) with special reference to diamides [J]. Pest Management Science, 2017, 73(6):1169-1178.
[27]	Lu Y H, Wang G R, Zhong L Q, et al. Resistance monitoring of Chilosuppressalis (Walker) (Lepidoptera: Crambidae) to chlorantraniliprole in eight field populations from east and central China [J]. Crop Protection, 2017, 100:196e202.

Insecticide Resistance Monitoring and Target Molecular Detection of Diamide Insecticides in Field Populations of Ostriniafurnacalis

亚洲玉米螟的抗药性监测及双酰胺类药剂抗性靶标分子检测

PDF

Knowledge

Abstract

Cite this article

share this article

References 27

Related Articles 15

Recommended Articles

Metrics

[1]	WANG Peng, ZHENG Kai, ZHAO Jieyin, GAO Wenju, LONG Yilei, CHEN Quanjia, QU Yanying. Evaluation and index screening of heat resistance of Gossypium hirsutum germplasm resources [J]. Xinjiang Agricultural Sciences, 2023, 60(9): 2081-2090.
[2]	QIN Pengliang, ZHOU Xiao, Kahsay Tadesse Mawcha, WANG Shuang, LI Jiaqi, LIU Ying, ZHANG Na, YANG Wenxiang. EMS mutants of wheat hengguan 35 and identification of the mutants resistant to fusarium crown rot [J]. Xinjiang Agricultural Sciences, 2023, 60(9): 2231-2238.
[3]	ZHANG Liguo, GUO Chungui, CHANG Lichun, GUO Xinlei, ZHANG Tao, WU Jian, LIANG Jianli, GAO Jie, WANG Xiaowu. Development of molecular markers for clubroot resistance and identification of germplasm resources for Brassica rapa [J]. Xinjiang Agricultural Sciences, 2023, 60(9): 2281-2290.
[4]	ZHANG Yongqiang, CHEN Chuanxin, NIE Shihui, XU Qijiang, Sailihan Sai, LEI Junjie. Regulation of chlormequat on lodging resistance of winter wheat stem during the period of drip application of CCC [J]. Xinjiang Agricultural Sciences, 2023, 60(8): 1873-1878.
[5]	SHAO Panxia, ZHAO Zhun, SHAO Wukui, HAO Xiaoyan, GAO Shengqi, LI Jianping, HU Wenran, HUANG Quansheng. Expression analysis of ZmCDPK22 gene in maize under drought stress [J]. Xinjiang Agricultural Sciences, 2023, 60(6): 1372-1378.
[6]	ZHANG Jingcan, ZHANG Yongqiang, LEI Junjie, CHEN Chuanxin, XU Qijiang, NIE Shihui, XU Wenxiu. Effects of different growth regulators on stem characteristics and lodging resistance of winter wheat under drip irrigation [J]. Xinjiang Agricultural Sciences, 2023, 60(5): 1067-1074.
[7]	TANG Bihui, ZHANG Lihua, LI Haiying, ZHANG Chong, JIANG Tinghao, ZHAO Xiaoyu, JIANG Teng, DING Yawen, WU Yingping, ZHAO Quanzhuang. A comparative study on reproductive performance, serum hormone levels and gene expression between Yili goose and hortobágy goose [J]. Xinjiang Agricultural Sciences, 2023, 60(5): 1271-1280.
[8]	YANG Minghua, LIU Qiang, LIAO Biyong, PEN Yuncheng, Buayxam Namat, Dawulai Jiekeshan. Comprehensive evaluation of lodging resistance of NCII maize combinations [J]. Xinjiang Agricultural Sciences, 2023, 60(4): 832-840.
[9]	SHANG Jing, PANG Hongbo, WANG Lanlan, LI Xuemei, WANG Yanqiu, LI Yueying. Study on the relationship between auxin and sorghum heterosis [J]. Xinjiang Agricultural Sciences, 2023, 60(4): 841-846.
[10]	TANG Xixi, Mubarek Ayup, XU Panyun, YU Qiuhong, GUO Chunmiao, ZHANG Ping, GONG Peng. Response of root anatomical structure of different rootstock resources of almond to drought stress [J]. Xinjiang Agricultural Sciences, 2023, 60(4): 897-907.
[11]	LIU Min, JIN Juan, Abudoukayoumu Ayimaiti, FAN Dingyu, HAO Qing, YANG Lei, ZHAO Xiaomei, GENG Wenjuan. Evaluation of cold resistance of three fresh edible jujube cultivars in Xinjiang [J]. Xinjiang Agricultural Sciences, 2023, 60(4): 916-924.
[12]	MA Qingqian, YANG Honglan, WEI Xin, ZHANG Dawei, Alisher A Abdullaev, CHENG Lihua, ZHANG Daoyuan. Identification and Screen from Eighteen Foreign Cotton Germplasm on Agronomy and Verticillium Resistance [J]. Xinjiang Agricultural Sciences, 2023, 60(2): 286-294.
[13]	LONG Tianyu, DENG Yahui, ZU Qianli, YANG Long, QU Yanying, CHEN Quanjia. Identification and Evaluation of Indoor Resistance to Cotton Fusarium wilt at Seedling Stage [J]. Xinjiang Agricultural Sciences, 2023, 60(2): 416-423.
[14]	JIA Yonghong, WEI Haipeng, HOU Dianliang, ZENG Chaowu, Nasirula Keremu, LIANG Xiaodong. Evaluation of correlation between drought resistance and agronomic traits of self-breeding spring wheat varieties in Xinjiang [J]. Xinjiang Agricultural Sciences, 2023, 60(12): 2940-2948.
[15]	WANG Dong, YI Haibo, LI Hongbo, QIN Lei, XU Qiqi, WU Huimin, XIA Lining. Drug resistance analysis of Enterococcus faecalis isolated from pigs and detection of related drug resistance genes in Aksu, Xinjiang [J]. Xinjiang Agricultural Sciences, 2023, 60(12): 3113-3120.