Identification of the Pathogen Causing Leaf Spot of Dianthus chinensis

doi:10.6048/j.issn.1001-4330.2021.04.014

Xinjiang Agricultural Sciences ›› 2021, Vol. 58 ›› Issue (4): 712-719.DOI: 10.6048/j.issn.1001-4330.2021.04.014

• Soil Fertilizer·Plant Protection·Microbes • Previous Articles Next Articles

Identification of the Pathogen Causing Leaf Spot of Dianthus chinensis

LI Fan, REN Yuzhong, JIAO Ruilian, SUN Lin, LI Guoying

Key Laboratory for Oasis Agricultural Pest Management and Plant Resource Utilization at Universities Levels of Xinjiang Uygur Autonomous Region/College of Agronomy, Shihezi University, Shihezi Xinjiang 832003,China

Received:2020-04-15 Online:2021-04-20 Published:2021-04-25
Correspondence author: REN Yuzhong(1970-),male,from Xinjiang,associate professor,doctor,research direction is plant disease epidemic and prevention research,(E-mail)404350315@qq.com
Supported by:
National Natural Science Foundation of China(31160357);the Project of Forestry Bureau XPCC(KC009903)

石竹叶斑病病原鉴定

李番, 任毓忠, 焦瑞莲, 孙璘, 李国英

新疆绿洲农业病虫害治理与植保资源利用自治区高校重点实验室/石河子大学农学院,新疆石河子 832003

通讯作者: 任毓忠(1970-),男,新疆人,副教授,研究方向为植物病害防治,(E-mail)404350315@qq.com
作者简介:李番(1995-),女,甘肃人,硕士研究生,研究方向为植物病理学,(E-mail)1986689330@qq.com
基金资助:
国家自然科学基金(31160357);兵团林业局项目(KC009903)

Abstract

Abstract: 【Objective】 The aim of this project is to clarify the pathogen of Dianthus chinensis leaf spot in Shihezi area and control the disease. 【Methods】 Using conventional organic law, 8 representative strains were selected and their pathogenicity was determined by mycelium patch and spray method. The morphological and rDNA-ITS regions, histone 3 and β- tubulin sequences were compared and analyzed to establish a multi-gene combined phylogenetic tree to determine the classification status of pathogens. 【Results】 Morphologically, the conidium was similar to Alternaria nobilis. The sequence analysis of rDNA-ITS and β-tubulin of 8 isolates were more than 99.0% homologous to the reported phylogeny of Alternaria nobilis in GenBank. 【Conclusion】 Combined with the morphological characteristics and molecular biological results, the pathogen causing the Dianthus chinensis leaf spot is identified as Alternaria. nobilis in Shihezi area.

Key words: Dianthus chinensis; multi-gene analysis; pathogen identification

摘要： 【目的】 研究鉴定引起新疆石河子地区石竹叶斑病的病原,为石竹叶斑病的防治提供理论基础。【方法】 采集典型石竹叶斑病发病叶片利用常规组织法分离和纯化,选取8个代表性菌株,采用菌丝块贴接法和喷雾法测定致病性;应用病菌形态学和rDNA-ITS区、组蛋白3和β-微管蛋白序列进行比对和分析,建立多基因联合系统发育树,确定病原菌的分类地位。【结果】 经形态学鉴定其分生孢子与Alternaria nobilis相似;供试菌株rDNA-ITS区和β-微管蛋白序列与已报道的石竹链格孢(A. nobilis)同源性高达99.0%以上,rDNA-ITS区和β-微管蛋白序列联合构建的系统发育树显示,8个代表菌株均与A. dianthi处于同一分支上,与其它链格孢亲缘关系较远。【结论】 引起石河子地区石竹叶斑病的病原菌为石竹链格孢Alternaria nobilis。

关键词: 石竹叶斑病, 多基因联合, 病原鉴定

CLC Number:

S432.9

LI Fan, REN Yuzhong, JIAO Ruilian, SUN Lin, LI Guoying. Identification of the Pathogen Causing Leaf Spot of Dianthus chinensis[J]. Xinjiang Agricultural Sciences, 2021, 58(4): 712-719.

李番, 任毓忠, 焦瑞莲, 孙璘, 李国英. 石竹叶斑病病原鉴定[J]. 新疆农业科学, 2021, 58(4): 712-719.

References 20

[1]	潘晓玲,皮锡铭.新疆石竹亚科的分布系统及区系的研究[J].干旱区研究,1992,(4):63-68.PAN Xiaoling, PI Ximing, Distribution system and flora of Dianthus chinensis subfamily in Xinjiang [J]. Arid Zone Research, 1992,(4):63-68.
[2]	李彪,董刚,马国辉,等.青藏高原甘南地区石竹科藏药植物资源调查研究[J].楚雄师范学院学报,2017,32(3):42-44, 50.LI Biao, DONG Gang, MA Guohui, et al. Investigation on traditional Tibetan medicinal plant resources of Caryophyllaceae in Gannan County of the Qinghai-Tibet plateau [J]. Journal of Chuxiong Normal University, 2017, 32(3):42-44, 50.
[3]	张东坡. 石竹的养护管理及病虫害防治技术[N]. 河北科技报,2017,5-20(007).ZHANG Dongpo. Maintenance and management of Dianthus chinensis disease and pest control technology [N]. Hebei Science and Technology Daily, 2017, 5-20(007).
[4]	赵兰枝,蒋学杰,孙丽,等. 新乡地区香石竹病害调查与病原鉴定[J].山东林业科技,2005,(1):33-34.ZHAO Lanzhi, JIANG Xuejie, SUN Li, et al. Disease investigation and disease factor appraisal of Carnation in Xinxiang [J]. Shandong Forestry Science and Technology, 2005,(1):33-34.
[5]	张健如,熊野林,陶庭典. 香石竹主要病害的危险性评价及其检疫规程初探[J].植物检疫,1993,(4):316-319.ZHANG Jianru, XIONG Yeling, TAO Tingdian. Risk assessment of major carnation diseases and preliminary study on quarantine procedures [J]. Plant Quarantine, 1993,(4):316-319.
[6]	郑红莉,冯淑杰,白成艳,等. 石竹茎腐病病原菌鉴定与生物学特性研究[J].安徽农业科学,2014,42(6):1674-1677. ZHENG Hongli, FENG Shujie, BAI Chengyan. et al. Identification of Pathogens and Researches of Theirs Biological Characteristics on Carnation Stem rot[J]. Journal of Anhui Agricultural Sciences, 2014, 42(6):1674-1677.
[7]	方中达.植病研究方法[M],北京:中国农业出版社,1998.FANG Zhongda. Plant Disease Study [M]. Beijing: China Agriculture Press, 1998.
[8]	胡翠凤,吴琼华. 香石竹叶斑病菌的生物学特性[J].福建农业大学学报,1997,(3):308-312.HU Cuifeng, WU Qionghua. Biological characteristics of carnation leaf spot fungus [J]. Journal of Fujian Agricultural University, 1997,(3):308-312.
[9]	张建宁.十字交叉法测定药剂对棉花黄萎病菌的抑制作用[J].基层农技推广,2015,(11).ZHANG Jianning. The inhibition effect of the agent on verticillium wilt of cotton was determined by cross - sectional method [J]. Extension of Agricultural Technology at the Grass-root Level, 2015,(11).
[10]	丁成. 北疆加工番茄早疫病病原生物学特性及防治研究[D].石河子:石河子大学硕士论文,2006.DING Cheng. Study on biological characteristics of Alternaria solani causing Processing tomato early blight and disease control in north of Xinjiang [D]. Shihezi: Shihezi University, 2006.
[11]	岳永亮,任毓忠,张莉,等. 新疆白菜黄萎病病原的鉴定[J]. 新疆农业科学,2015,52(12):2264-2267.YUE Yongliang,REN Yuzhong,ZHANG Li, et al. Identification of the Pathogen Causing Chinese Cabbage Verticillium wilt in Xinjiang [J]. Xinjiang Agricultural Sciences, 2015, 52(12):2264-2267.
[12]	刘威,袁丁,郭桂义,等. 茶树炭疽病病原鉴定[J].南方农业学报,2017,48(3):448-453.LIU Wei, YUAN Ding, GUO Guiyi, et al. Identification of anthracnose pathogen in tea plant[J]. Journal of Southern Agriculture, 2017,48(3):448-453.
[13]	胡翠凤,陈仲豪. 福州香石竹叶斑病病原鉴定[J].福建农业大学学报,1996,(4):70-72.HU Cuifeng, CHEN Zhonghao. Identification of the Pathogen Causing carnation leaf spot in Fuzhou [J]. Journal of Fujian Agricultural University, 1996,(4):70-72.
[14]	Garibaldi, A., et al. First Report of Leaf Spot of Saponaria officinalis Caused by Alternaria nobilis in Italy [J]. Plant Disease, 2013,97(3):424-424.
[15]	Ahmet ASAN. Checklist of Alternaria Species Reported From Turkey [J]. The Journal of Fungus, 2015, 6(2)43-57.
[16]	Simmons, E. G. Alternaria themes and variations (287-304) species on caryophyllaceae [J]. Mycotaxon, 2002,(82): 1-40.
[17]	孙俊,刘志恒,黄欣阳,等. 辣椒褐斑病菌分生孢子产生条件初探[J].植物病理学报,2010,40(3): 322-324.SUN JUN, LIU Zhiheng, HUANG Xinyang, et al. A preliminary study on the sporulation conditions of Cercospora capsica [J]. Acta Phytophylacica Sinica, 2010, 40(3):322-324.
[18]	Takeshi Horia. Patuogenicity temperature and pathogen coverage of carnation [J]. Ann. Rept. Plant Prot. Noth. Japan, 1998, 49:96-99.
[19]	张能唐,胡晓艳.香石竹叶斑病防治研究[J].植物保护学报,1982,(2):125-130, 147.ZHANG Nengtang, HU Xiaoyan. Study on prevention and treatment of carnation leaf spot [J]. Journal of Plant Protection, 1982,(2):125-130, 147.
[20]	Duan, Chun Fang, et al. First report of Alternaria dianthicola causing flower blight on carnation in China [J]. EPPO Bulletin, 2015, 45(2):195-198.

Identification of the Pathogen Causing Leaf Spot of Dianthus chinensis

石竹叶斑病病原鉴定

PDF

Knowledge

Abstract

Cite this article

share this article

References 20

Related Articles 4

Recommended Articles

Metrics

[1]	ZHAO Zhiqiang, ZHANG Luhui, ZHAO Quanxin, GUO Qingyuan. Identification of Pathogen Causing Sugar Beet Gibellulopsis Yellow Wilt Based on Multiple Gene Sequence Analysis [J]. Xinjiang Agricultural Sciences, 2022, 59(2): 425-432.
[2]	MA Dun;WANG Feng-bin;WU Qiang;ZHANG Yan-hong;YUAN Jie;Hailiqiemu;ZHAO Zhi-qiang;CAO Wei. Studies on Identification and Control of Rice Spikelet Rot Disease (RSRD) Pathogens Species in Xinjiang [J]. , 2017, 54(6): 1093-1098.
[3]	Shanawaer Semaiti;Yushanjiang Maimaiti;GUO Qing-yuan;BAI Jian-yu. Identification of the Pathogen Causing Jujube Fruit Mildew (Part Ⅱ)——Isolation and Identification of Penicillium fungus Causing Jujube Fruit Mildew [J]. , 2016, 53(4): 698-705.
[4]	Shanawaer Semaiti;Yushanjiang Maimaiti;GUO Qing-yuan;BAI Jian-yu. Identification of the Pathogen Causing Jujube Fruit Mildew (Part Ⅰ)-Isolation and Identification of Aspergillus fungus Causing Jujube Fruit Mildew [J]. , 2016, 53(3): 502-509.