Evaluation of Field Resistance of 180 Tomato Germplasm Resources to Three Major Leaf Diseases

doi:10.6048/j.issn.1001-4330.2021.04.017

Xinjiang Agricultural Sciences ›› 2021, Vol. 58 ›› Issue (4): 741-755.DOI: 10.6048/j.issn.1001-4330.2021.04.017

• Soil Fertilizer·Plant Protection·Microbes • Previous Articles Next Articles

Evaluation of Field Resistance of 180 Tomato Germplasm Resources to Three Major Leaf Diseases

LI Guohua¹, WANG Xiaomin^1,2,3,4, ZHAO Yufei¹, HU Xinhua⁵, FU Jinjun⁵, GAO Yanming^1,2,3,4, LI Jianshe^1,2,3,4

1. School of Agriculture, Ningxia University, Yinchuan 750021, China;
2. Key Laboratory of Modern Molecular Breeding for Dominant and Special Crops in Ningxia, Yinchuan 750021, China;
3. Ningxia Modern Facility Horticulture Engineering Technology Research Center, Yinchuan 750021, China;
4. Ningxia Facility Horticulture(Ningxia University)Technology Innovation Center, Yinchuan 750021, China;
5. Ningxia Jufeng Seedlings Co., Ltd., Yinchuan 750021, China

Received:2020-04-20 Online:2021-04-20 Published:2021-04-25
Correspondence author: WANG Xiaomin(1981-), female, associate professor, research area: vegetable biotechnology and genetic breeding, (E-mail)wangxiaomin_1981@163.com
Supported by:
Ministry of Education Chunhui Plan 2016 and Special Agricultural Breeding Project of Nixia Hui Autonomous Region (NXNYYZ20150303)

180份番茄种质资源对3种主要叶部病害的田间抗病性评价

李国花¹, 王晓敏^1,2,3,4, 赵宇飞¹, 胡新华⁵, 付金军⁵, 高艳明^1,2,3,4, 李建设^1,2,3,4

1.宁夏大学农学院,银川 750021;
2.宁夏优势特色作物现代分子育种重点实验室,银川 750021;
3.宁夏现代设施园艺工程技术研究中心,银川 750021;
4.宁夏设施园艺(宁夏大学)技术创新中心,银川 750021;
5.宁夏巨丰种苗有限责任公司,银川 750021

通讯作者: 王晓敏(1981-),女,陕西宝鸡人,副教授,研究方向为蔬菜生物技术与遗传育种,(E-mail) wangxiaomin_1981@163.com
作者简介:李国花(1995-),男,山西忻州人,硕士研究生,研究方向为蔬菜生物技术与遗传育种,(E-mail)1546585843@qq.com
基金资助:
2016年度教育部春晖计划;宁夏回族自治区农业育种专项(NXNYYZ20150303)

Abstract

Abstract: 【Objective】 Under the natural disease conditions in the solar greenhouse, 180 tomato germplasm resources are identified for disease resistance in order to screen multi-resistance materials and lay a foundation for the breeding of multi-resistance tomato in Ningxia. 【Methods】 The disease types were determined according to the disease symptoms, and the disease incidence and disease index were used to determine the disease resistance grade of 180 tomato germplasm resources to the late blight, leaf mold and early blight by 5-point sampling method. Finally, the fuzzy membership function method was used to evaluate comprehensively the disease resistance of germplasm materials. 【Results】 All the 179 tomato materials had late blight, leaf mold and early blight, and only 1 material had developed late blight and leaf mold, but not early blight. The incidence of late blight was the most serious, followed by leaf mold, the incidence of early blight was the lightest. There were 5, 25 and 50 materials with above “resistance” grade to the late blight, leaf mold and early blight, respectively, which accounted for 2.78%, 13.89% and 27.22% of the tested materials. 16 materials were screened out, all of which reached the “middle resistance” grade to each of the three diseases. Materials 63049 and 63054 ranked the top 2 in the comprehensive resistance, and the average value of membership function was 0.98 and 0.93 respectively. 【Conclusion】 These two materials,63049 and 63054, are all above the disease resistance grade and have excellent comprehensive resistance. They can be used as multi-resistant tomato germplasm resources.

Key words: tomato; germplasm resources; leaf disease; disease resistance; evaluation

摘要： 【目的】 在日光温室内田间自然发病条件下,鉴定180份番茄种质资源进行抗病性,筛选多抗材料,为宁夏番茄多抗品种选育奠定基础。【方法】 对180份番茄种质资源采用5点式取样法,根据病害症状确定病害的类别,调查发病率和病情指数,研究其对晚疫病、叶霉病、早疫病3种主要叶部病害的抗病性等级,利用模糊隶属函数法,综合评价种质材料抗病性。【结果】 179份番茄材料全部发生晚疫病、叶霉病、早疫病,仅1份材料只发生晚疫病和叶霉病,未发生早疫病。晚疫病发病最严重,其次为叶霉病,早疫病发病最轻。对晚疫病、叶霉病和早疫病达到抗病等级以上的材料分别有5、25和50份,占供试材料的2.78%、13.89%和27.22%。筛选出16份材料对3种叶部病害均达到中抗等级以上。材料63049和63054综合抗性排名位列前2,隶属函数平均值分别为0.98和0.93。【结论】 63049和63054这2份材料对这3种病害均达到抗病等级以上且综合抗性好,可作为多抗番茄种质资源。

关键词: 番茄, 种质资源, 叶部病害, 抗病性, 评价

CLC Number:

S436.412

LI Guohua, WANG Xiaomin, ZHAO Yufei, HU Xinhua, FU Jinjun, GAO Yanming, LI Jianshe. Evaluation of Field Resistance of 180 Tomato Germplasm Resources to Three Major Leaf Diseases[J]. Xinjiang Agricultural Sciences, 2021, 58(4): 741-755.

李国花, 王晓敏, 赵宇飞, 胡新华, 付金军, 高艳明, 李建设. 180份番茄种质资源对3种主要叶部病害的田间抗病性评价[J]. 新疆农业科学, 2021, 58(4): 741-755.

References 21

[1]	李天来. 设施蔬菜栽培学[M]. 北京: 中国农业大学出版社, 2011.LI Tianlai. Facilities VegeTable Cultivation [M]. Beijing: China Agricultural University Press, 2011.
[2]	吴晓燕.宁夏温室番茄主要病害的发生与综合防治[D].杨凌:西北农林科技大学,2004.WU Xiaoyan. Occurrence and integrated control of main diseases of tomato in greenhouse in Ningxia [D]. Yangling: Northwest Agriculture & Forestry University, 2004.
[3]	刘世伟.宁夏永宁县设施番茄病害发生情况调查及防治策略探讨[D].杨凌: 西北农林科技大学,2018.LIU Shiwei. Investigation and control strategy of tomato plant disease in Yongning county, Ningxia [D]. Yangling: Northwest Agriculture & Forestry University, 2018.
[4]	司天桃,张国强,薛琳,等.加工番茄叶霉病的防治药剂筛选及其品种抗性鉴定[J].植物保护,2017,43(5):225-228.SI Tiantao, ZHANG Guoqiang, XUE Lin, et al. Screening of antifungal agents and identification of resistant variety against leaf mold of processed tomato [J]. Plant Protection, 2017, 43(5):225-228.
[5]	尚春明,高振江,李秀华,等.番茄早疫病抗性材料的苗期接种鉴定[J].北方园艺,2018,(14):1-5.SHANG Chunming, GAO Zhenjiang, LI Xiuhua, et al. Inoculation identification of tomato germplasm resistance to early blight seedling stage [J]. Northern Horticulture, 2018,(14):1-5.
[6]	何伟,杨华,崔元玗,等.新疆喀什地区设施番茄晚疫病调查及品种抗病性鉴定[J].新疆农业科学,2014,51(11):2025-2029.HE Wei, YANG Hua, CUI Yuanyu, et al. Survey of tomato Late blight and identification of resistance of cultivars in greenhouse in Kashi region of Xinjiang [J]. Xinjiang Agricultural Sciences, 2014, 51(11):2025-2029.
[7]	张英杰,张举梅,张骞红.番茄叶霉病苗期抗病性鉴定方法[J].北方园艺,1998,(3):41-42.ZHANG Yingjie, ZHANG Jumei, ZHANG Qianhong. Method for identifying disease resistance of tomato leaf mold at seedling stage [J]. Northern Horticulture, 1998,(3):41-42.
[8]	许向阳.番茄苗期多抗性鉴定方法研究及抗病种质资源筛选[D].哈尔滨: 东北农业大学,2000.XU Xiangyang. Study on the methods of identification for multiple disease resistance in tomato seedling and the screening of resistant source of tomato [D]. Harbin: Northeast Agricultural University, 2000.
[9]	王彦杰,刘守伟,李景富.番茄抗早疫病育种研究进展[J].东北农业大学学报,2004,35(5):612-621.WANG Yanjie, LIU Shouwei, LI Jingfu. Researching advancement in breeding of tomato resistance to early blight [J]. Journal of Northeast Agricultural University, 2004, 35(5):612-621.
[10]	张子君,邹庆道,李海涛,等.番茄早疫病抗病性鉴定研究[J].北方园艺,2005,(1):53-55.ZHANG Zijun, ZOU Qingdao, LI Haitao, et al. Identification of disease resistance of tomato early blight [J]. Northern Horticulture, 2005,(1):53-55.
[11]	薛敏菊,李宝聚,傅俊范.番茄晚疫病研究进展[J].沈阳农业大学学报,2002,33(6):456-460.XUE Minju, LI Baoju, FU Junfan, et al. Research progress in tomato Late blight[J]. Journal of Shenyang Agricultural University, 2002,33(6):456-460.
[12]	苏银玲,杨子祥,麻继仙,等.41份番茄资源对3种番茄主要叶部病害的抗性鉴定[J].南方农业学报, 2014, 45(7): 1197-1200.SU Yinling, YANG Zixiang, MA Jixian, et al. Resistance of 41 tomato resources against three foliar diseases[J].Journal of Southern Agriculture, 2014, 45(7):1197-1200.
[13]	李锡香,杜永臣.番茄种质资源描述规范和数据标准[M].北京:中国农业出版社, 2006.LIX Xixiang, DU Yongchen. Descriptors and data standard for tomato [M]. Beijing: China Agriculture Press, 2006.
[14]	芮文婧,王晓敏,张倩男,等.47份大果番茄种质资源表型性状的遗传多样性[J].江苏农业科学,2017,45(12):92-95.RUI Wenjing, WANG Xiaomin, ZHANG Qiannan, et al. Genetic diversity analysis of 47 tomato germplasm resources by phenotypic traits[J]. Jiangsu Agricultural Sciences, 2017,45(12):92-95.
[15]	温晓涵,张喜春.引进番茄品种抗晚疫病苗期鉴定及抗性品种筛选[J].中国农学通报,2010,26(4):189-194.WEN Xiaohan, ZHANG Xichun. The identification of introduced tomato varieties Phytophora infestans (Mont.) De Bary in the detached sage sequent and varieties selection [J]. Chinese Agricultural Science Bulletin, 2010, 26(4):189-194.
[16]	张振粉,杨成德,陈秀荣,等.甘肃省番茄早疫病菌致病力测定及品种田间抗病性鉴定[J].甘肃农业大学学报,2010,12(6):114-117.ZHANG Zhenfen, YANG Chengde, CHEN Xiurong, et al. Pathogenicity determination and species identification of field disease resistance against tomato early blight (Alternaria soLateni) in Gansu Province[J].Journal of Gansu Agricultural University,2010,12(6):114-117.
[17]	王志泰, 马瑞, 马彦军, 等. 利用隶属函数法分析胡枝子抗旱性[J].干旱区资源与环境,2013,27(9):119-123.WANG Zhitai, MA Rui, MA Yanjun, et al. The drought resistance of 5 species of Lespedza [J]. Journal of Arid Land Resources and Environment, 2013, 27(9):119-123.
[18]	王进明. 张掖市加工番茄白粉病的研究[D].兰州:甘肃农业大学, 2009.WANG Jinming. Study on tomato powdery mildew in Zhangye City [D]. Lanzhou: Gansu Agricultural University, 2009.
[19]	李元龙, 王中华. 分子标记技术在作物育种中的应用与展望[J].河南师范大学学报(自然科学版), 2016, 44(3): 140-145, 183.LI Yuanlong, WANG Zhonghua. Application and perspective of molecular marker technology in crop breeding [J].Journal of Henan Normal University (Natural Science Ed.), 2016, 44(3): 140-145, 183.
[20]	GAO Caixia. Precision plant breeding using genome editing technologies [J].Transgenic Research, 2019, (28): 53-55.
[21]	霍晋彦,李姣,荆雅峰, 等.CRISPR/Cas9系统在植物基因功能研究中的应用进展[J].植物生理学报,2019,55(3):241-246.HUO Jinyan, LI Jiao, JING Yafeng, et al. Progress on the application of CRISPR/Cas9 system in the functional study of plant genes [J]. Plant Physiology Journal, 2019, 55(3): 241-246.

Evaluation of Field Resistance of 180 Tomato Germplasm Resources to Three Major Leaf Diseases

180份番茄种质资源对3种主要叶部病害的田间抗病性评价

PDF

Knowledge

Abstract

Cite this article

share this article

References 21

Related Articles 15

Recommended Articles

Metrics

[1]	WANG Peng, ZHENG Kai, ZHAO Jieyin, GAO Wenju, LONG Yilei, CHEN Quanjia, QU Yanying. Evaluation and index screening of heat resistance of Gossypium hirsutum germplasm resources [J]. Xinjiang Agricultural Sciences, 2023, 60(9): 2081-2090.
[2]	LIU Yufang, ZHANG Zhigang, LI Changcheng, LI Hong, CHENG Ping, YANG Lu. Effects of different temperature and maturity on rot rate and quality of apricot during storage [J]. Xinjiang Agricultural Sciences, 2023, 60(9): 2189-2197.
[3]	SONG Bingmei, JIANG Yan, CHEN Xin, ZHANG Yu, CHENG Wannan, PAN Hongsheng. Evaluation of saline/alkali tolerance of new transgenic High-Yield cotton at germination and seedling stages [J]. Xinjiang Agricultural Sciences, 2023, 60(9): 2239-2247.
[4]	WANG Dandan, LI Yan, ZHANG Qingyin, Li Shidong, PANG Yongchao, MA Kunzhi, MA Long, NIU Ruisheng, ZHONG Zengming, QI Lianfen, SHI Jianhua. Effects of different microbial treatments on tomato soil microbial diversity [J]. Xinjiang Agricultural Sciences, 2023, 60(9): 2248-2257.
[5]	PU Min, RUAN Xiangyang, XIAO Lele, SUO Changkai, CHEN Guoyong, YE Jun, GAO Bo. Effect of citric acid soluble calcium magnesium fertilizer on calcium and magnesium absorption and quality of processing tomato [J]. Xinjiang Agricultural Sciences, 2023, 60(8): 1987-1995.
[6]	XIAO Lingan, MA Yan, SONG Bingwei, JIAO Ruibin, XING Jianfei. Research status and progress of micro-jet irrigation technology under plastic film in greenhouses [J]. Xinjiang Agricultural Sciences, 2023, 60(7): 1731-1740.
[7]	QU Kejia, SHI Xiaolei, ZHANG Heng, WANG Xingzhou, GENG Hongwei, DING Sunlei, ZHANG Jinbo, YAN Yongliang. Evaluation of drought resistance of introduced spring wheat under PEG treatment [J]. Xinjiang Agricultural Sciences, 2023, 60(6): 1363-1371.
[8]	LIU Jiangna, ZHANG Xiying, LI Rongxia, ZHANG Xiaowei, BAI Yunfeng, ZHANG Aiping. Molecular characteristics and promoter analysis of SlLCY-B2 gene in tomato [J]. Xinjiang Agricultural Sciences, 2023, 60(6): 1460-1465.
[9]	ZHANG Yanhong, HOU Tianyu, BA Yinhua, ZHAO Caiyue, LYU Yuping, Buhalikeimu Abunzi, ZHAO Zhiqiang, LI Dong, DU Xiaojing, YUAN Jie, WANG Fengbin. Identification and evaluation of salt tolerance of rice recombinant inbred lines at bud and seedling stages [J]. Xinjiang Agricultural Sciences, 2023, 60(5): 1041-1049.
[10]	HUANG Qiannan, Maerheba Aisibaier, ZOU Hui, WANG Cairong, Ailimaimaiti Kuerban, SUN Na, LEI Junjie. Genetic diversity of main agronomic traits in Xinjiang winter wheat germplasm resources [J]. Xinjiang Agricultural Sciences, 2023, 60(5): 1050-1058.
[11]	SANG Zhiwei, LIANG Yajun, GONG Zhaolong, ZHENG Juyun, WANG Junduo, LI Xueyuan, CHEN Quanjia. Analysis of mechanical harvesting characters of germplasm resources of different upland cotton [J]. Xinjiang Agricultural Sciences, 2023, 60(5): 1088-1098.
[12]	LU Tao, ZENG Qingtao, ZHANG Wen, WANG Wenbo, WANG Zhengyang, YANG Rui, SUN Yuyan. Comprehensive evaluation of cotton yield and quality by principal component analysis and grey correlation analysis [J]. Xinjiang Agricultural Sciences, 2023, 60(5): 1099-1109.
[13]	Halidan Yikeremu, LIU Na, LIU Lianzheng, ZHOU Anding, JIANG Qiyan, Damailijiang Hezier, CAO Junmei, ZHANG Xinzhong. Identification and evaluation of salt tolerance in wheat relatives at germination and seedling stages [J]. Xinjiang Agricultural Sciences, 2023, 60(5): 1118-1126.
[14]	YAO Qing, Aribelegan Hazzetti, YANG Minghua, LI Qiang, MIAO Haocui, CUI Hongliang. Response of quinoa seeds to germination temperature and identification of germination ability under low temperature stress [J]. Xinjiang Agricultural Sciences, 2023, 60(5): 1141-1149.
[15]	YANG Minghua, LIU Qiang, LIAO Biyong, PEN Yuncheng, Buayxam Namat, Dawulai Jiekeshan. Comprehensive evaluation of lodging resistance of NCII maize combinations [J]. Xinjiang Agricultural Sciences, 2023, 60(4): 832-840.