Effects of Exogenous Calcium on Physiological Characteristics of Muskmelon Seedling Leaves during High Temperature Period

doi:10.6048/j.issn.1001-4330.2022.03.008

Abstract

Abstract:

【Objective】 To study the effects of different concentrations of CaCl₂ on the morphological changes and physiological characteristics of muskmelon seedlings under high temperature and strong light in autumn field in Turpan area, so as to provide reference for determining the optimal application concentration of CaCl₂, and improving the stress resistance of muskmelon seedlings and the whole seedling and strong seedling rate of melon. 【Methods】 Four treatments were set up to study the physiological characteristics of muskmelon Xizhoumi 17, which were sprayed with 20, 10 and 5 mmol/L, respectively. The effects of different concentrations of CaCl₂ on physiological characteristics of muskmelon seedlings were analyzed. 【Results】 Under high temperature condition, with the growth and exercise of seedlings, the soluble protein content of muskmelon leaves showed a three-stage "S" curve of "slow decline-rapid rise-slow rise", the relative content of chlorophyll always increased, and the antioxidant enzyme activities (SOD, POD, CAT) and MDA content first increased and then decreased. Soluble protein content, antioxidant enzyme activities (SOD, POD, CATt) and MDA content increased significantly, then decreased rapidly from 3-leaf stage to 5-leaf stage, and slowly decreased or fluctuated in a certain range from 5-leaf stage to 9-leaf stage. 【Conclusion】 Spraying 5 mmol/L CaCl₂ can increase the relative content of chlorophyll and soluble protein by 17.70% and 23.61% respectively, and the activities of SOD, pod and cat are increased by 115.79%, 57.42% and 53.52% respectively, and the content of MDA is decreased by 22.13% compared with the control.

Key words: heat stress; melon seedlings; antioxidant enzymes; heat tolerance

摘要：

【目的】研究不同浓度CaCl₂对秋季大田高温强光下甜瓜苗期的形态变化和生理特性的影响,为秋季大田甜瓜生产苗期管理确定CaCl₂最佳施用浓度、提高甜瓜幼苗的抗逆性和甜瓜全苗、壮苗率提供参考。【方法】以大田主栽品种西州密17号甜瓜为试材,设4个处理,以喷施清水为对照(CK),喷施20、10、5 mmol/L 3个浓度的CaCl₂,测定甜瓜苗期的生理特性指标,分析不同CaCl₂浓度处理对甜瓜幼苗生理特性的影响。【结果】在外界高温条件下,随着幼苗的生长和锻炼,甜瓜叶片可溶性蛋白含量总体呈“缓慢下降-迅速上升-缓慢上升”的三段式“S”曲线变化,叶绿素相对含量始终上升,抗氧化酶活性(SOD、POD、CAT)和MDA含量呈先升后降的趋势;从甜瓜幼苗1叶期至3叶期间可溶蛋白含量、抗氧化酶活性(SOD、POD、CAT)和MDA含量显著升高,在3叶期至5叶期之间均迅速下降,5叶期至9叶期之间缓慢下降或一定范围波动。【结论】高温环境下,喷施5 mmol/L CaCl₂,叶绿素相对含量和可溶蛋白含量分别比对照处理高17.70%和23.61%,SOD、POD、CAT活性分别比对照提高115.79%、57.42%、53.52%,MDA含量比对照降低22.13%,提高甜瓜幼苗耐热性效果显著。

关键词: 热胁迫, 甜瓜幼苗, 抗氧化酶, 耐热性

CLC Number:

S652

LIU Zhigang, REN Hongsong, Huxidan Maimaiti, WANG Ruihua, LI Haifeng, HU Guozhi. Effects of Exogenous Calcium on Physiological Characteristics of Muskmelon Seedling Leaves during High Temperature Period[J]. Xinjiang Agricultural Sciences, 2022, 59(3): 588-596.

刘志刚, 任红松, 胡西旦·买买提, 王瑞华, 李海峰, 胡国智. 高温期喷施外源钙对甜瓜幼苗叶片生理特性的影响[J]. 新疆农业科学, 2022, 59(3): 588-596.

Figures/Tables 7

Fig.1 Variation of daily maximum temperature from July to August in 2018 and 2019

Fig.2 Effects of different concentrations of CaCl2 on chlorophyll content in leaves of Muskmelon seedlings

Fig.3 Effects of different concentrations of CaCl2 on solube protein content in leaves of Muskmelon seedlings

Fig.4 Effects of different concentrations of CaCl2 on CAT activity in leaves of Muskmelon seedlings

Fig.5 Effects of different concentrations CaCl2 on SOD activity in leaves of Muskmelon seedlings

Fig.6 Effects of different concentrations CaCl2 on POD activity of leaves of Muskmelon seedlings

Fig.7 Effects of different concentractions CaCl2 on MDA actiyity of Muskmelon seedlings

References 27

[1]	林德佩, 吴大康, 齐三魁. 中国甜瓜[M]. 北京: 科学普及出版社, 1991.
	LIN Depei, WU Dakang, QI Sankui. Melon [M]. Beijing: Science Popularization Press, 1991.
[2]	刘鸿先, 曾韶西, 李平. 植物抗寒性与酶系统多态型的关系[J]. 植物生理学通讯, 1985, (6): 6-11.
	LIU Hongxian, ZENG Shaoxi, LI Ping. Relation on the plant chilling resistance and enzyme system polymorphism[J]. Plant Physiol. Communications, 1981,(6): 6-11.
[3]	张永平, 陈幼源, 杨少军. 高温胁迫对不同甜瓜品种幼苗生长和生理特性的影响[J]. 上海农业学报, 2011, 27(2): 71-76.
	ZHANG Yongping, CHEN Youyuan, YANG Shaojun. Effects of high temperature stress on seedling growth and physiological characteristics of different melon varieties[J]. Shanghai Agricultural Journal, 2011, 27(2): 71-76.
[4]	杨少军, 张永平, 陈幼源. 高温胁迫对不同甜瓜品种的种子萌发及幼苗生长和抗氧化酶活性的影响[J]. 种子, 2012, 31(7): 86-88, 92.
	YANG Shaojun, ZHANG Yongping, CHEN Youyuan. Effects of high temperature stress on seed germination, seedling growth and antioxidant enzyme activities of different melon varieties[J]. Seeds, 2012, 31(7): 86-88, 92.
[5]	金春燕, 郭世荣, 施标, 等. 高温对不同番茄品种幼苗叶片抗氧化系统和渗透调节物质的影响[J]. 上海农业学报, 2011, 27(3): 26-30.
	JIN Chunyan, GUO Shirong, SHI Biao, et al. Effects of high temperature on antioxidant system and Osmoregulation Substances in leaves of different tomato varieties[J]. Shanghai Agricultural Journal, 2011, 27(3): 26-30.
[6]	Batistic O, Kudlaj . Calcium: not just another ion[M]. Springer: Cell Biology of Metals and Nutrients, 2010:17-54.
[7]	Allakhverdiev Si, Kreslavski V, Klimov V, et al. Heatstress: An overview of molecular responses in photosynjournal[J]. Photosynjournal Research, 2008,(98): 541-550.
[8]	Bhattachajee S. Calcium-dependent signaling pathway in the heat-induced oxidative injury in Amaranthuslividus[J]. Biologia Plantarum, 2008, 52(1): 137-140. DOI URL
[9]	李德红, 王小青, 潘瑞炽. 钙信使系统与植物激素信号传递[J]. 生物学杂志, 1998, 15(4): 1-4.
	LI Dehong, WANG Xiaoqing, PAN Ruichi. Calcium messenger system and plant hormone signaling[J]. Journal of Biology, 1998, 15(4): 1-4.
[10]	孙大业, 郭艳林. 细胞信号系统[M]. 北京: 科学出版社, 1991:140-203.
	SUN Daye, GUO Yanlin. Cell signaling system [M]. Beijing: Science Press, 1991:140-203.
[11]	梁永富, 王康才, 隋利, 等. Ca²⁺对高温强光胁迫下多花黄精抗逆生理和光合特性的影响[J]. 生态学杂志, 2018, 37(8):2345-2353.
	LIANG Yongfu, WANG Kangcai, SUI Li, et al. Effects of Ca²⁺ on stress resistance physiology and photosynthetic characteristics of Polygonatummultiflorum under high temperature and strong light stress[J]. Journal of Ecology, 2018, 37(8): 2345-2353.
[12]	张宗申, 利容千, 王建波. 外源Ca²⁺预处理对高温迫下辣椒叶片细胞膜透性和GSH、ASA含量及Ca²⁺分布的影响[J]. 植物生态学报, 2001, 25(2): 230-234.
	ZHANG Zongshen, LI Rongqian, WANG Jianbo. Effects of exogenous Ca²⁺ pretreatment on cell membrane permeability, GSH, ASA contents and Ca²⁺ distribution in Pepper Leaves under high temperature stress[J]. Actaphytoecology, 2001, 25(2): 230-234.
[13]	Gong M, Vander Luit A H,. Knight M R,. Trewavas A J. Heat-shock-induced changes in intracellular Ca²⁺ level in tobacco seedlings in relation to thermo tolerance[J]. Plant Physiology, 1998, (116): 429-437.
[14]	宰学明, 吴国荣, 钦佩, 等. Ca²⁺对花生幼苗耐热性的影响[J]. 南京林业大学学报(自然科学版), 2006,(3): 47-50.
	ZAI Xueming, WU Guorong, QIN Pei, et al. Effect of Ca²⁺ on heat tolerance of peanut seedlings[J]. Journal of Nanjing Forestry University (Natural Science Edition), 2006,(3): 47-50.
[15]	Gong M, Li Y J, Dai M, et al. Involvement of calcium and almodulin the acquisution of heat-shock inducedthermotolerance in Maize[J]. Journal of Plant Physiol, 1997, 150(5): 615-621. DOI URL
[16]	郑秋玲, 谭伟, 马宁. 钙对高温下巨峰葡萄叶片光合作用和叶绿素荧光的影响[J]. 中国农业科学, 2010, 43(9): 1963-1968.
	ZHENG Qiuling, TAN Wei, MA Ning. Effects of calcium on Photosynjournal and chlorophyll fluorescence of Kyoho Grape Leaves under high temperature[J]. Chinese Journal of Agricultural Sciences, 2010, 43(9): 1963-1968.
[17]	齐明芳, 王丹, 齐红岩, 等. 钙处理对高温胁迫下番茄幼苗光合及钙含量的影响[J]. 沈阳农业大学学报, 2015, 46(3): 277-283.
	QI Mingfang, WANG Dan, QI Hongyan, et al. Effects of calcium treatment on Photosynjournal and calcium content of Tomato Seedlings under high temperature stress[J]. Journal of Shenyang Agricultural University, 2015, 46(3): 277-283.
[18]	李天来, 李森, 孙周平. 钙和水杨酸对亚高温胁迫下番茄叶片保护酶活性的调控作用[J]. 应用生态学报, 2009, 2(3): 586-590.
	LI Tianlai, LI Sen, SUN Zhouping. Regulation of calcium and salicylic acid on protective enzyme activity of tomato leaves under sub high temperature stress[J]. Acta Applied Ecology, 2009, 2(3): 586-590.
[19]	新疆西瓜甜瓜资源调查组. 新疆甜瓜西瓜志[M]. 乌鲁木齐: 新疆人民出版社, 1985.
	Xinjiang Watermelon and Melon Resources Investigation Group. Xinjiang melon and watermelon records[M]. Urumqi: Xinjiang people's publishing house, 1985.
[20]	李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000.
	LI Hesheng. Principles and techniques of plant physiological and biochemical experiments [M]. Beijing: Higher Education Press, 2000.
[21]	汪祖程, 徐跃进. 黄瓜耐热性研究进展[J]. 长江蔬菜, 2008,(9): 32-37.
	WANG Zucheng, XU Yuejin. Research progress on heat resistance of cucumber[J]. Changjiang Vegetable, 2008, (9): 32-37.
[22]	杨秋珍, 李军, 王军霞, 等. 高温胁迫下甜瓜生理生态特性的研究[J]中国生态农业学报, 2003,(1): 26-28.
	YANG Qiuzhen, LI Jun, WANG Junxia, et al. Physiological and ecological characteristics of Muskmelon under high temperature stress[J] Chinese Journal of Ecological Agriculture, 2003,(1): 26-28.
[23]	程琳, 赵立群, 吴新新, 等. 不同苗龄期黄瓜根系生理生化及形态指标的研究[J]. 中国农学通报, 2012, 28(1): 181-186.
	CHENG Lin, ZHAO Liqun, WU Xinxin, et al. Study on physiological, biochemical and morphological indexes of cucumber roots at different seedling ages[J]. Chinese Agronomic Bulletin, 2012, 28(1): 181-186.
[24]	张红梅, 金海军, 丁小涛, 等. 黄瓜幼苗对热胁迫的生理反应及叶绿素荧光特性[J]. 上海交通大学学报(农业科学版), 2011, 29(5): 61-66.
	ZHANG Hongmei, JIN Haijun, DING Xiaotao, et al. Physiological response and chlorophyll fluorescence characteristics of cucumber seedlings to heat stress[J]. Journal of Shanghai Jiaotong University (Agricultural Science Edition), 2011, 29(5): 61-66.
[25]	孟焕文, 张彦峰, 程智慧. 黄瓜幼苗对热胁迫的生理反应及耐热鉴定指标筛选[J]. 西北农业学报, 2000, 9(1): 96-99.
	MENG Huanwen, ZHANG Yanfeng, CHENG Zhihui. Physiological response of cucumber seedlings to heat stress and screening of heat resistance identification indexes[J]. Northwest Agricultural Journal, 2000, 9(1): 96-99.
[26]	Jiang Y, Huang B. Plants and the environment. Effects of calcium on antioxidant activities and water relations associated with heat tolerance in two cool-season grasses[J]. Journal of Experimental Botany, 2001, (52): 341-349.
[27]	涂三思, 秦天才. 高温胁迫对黄姜叶片脯氨酸、可溶性糖和丙二醛含量的影响[J]. 湖北农业科学, 2004,(4): 98-100.
	TU Sansi, QIN Tiancai. Effects of high temperature stress on proline, soluble sugar and malondialdehyde contents in leaves of Dioscorea zingiberensis[J]. Hubei Agricultural Sciences, 2004,(4): 98-100.