Effects of urease/nitrification inhibitors on nutrient absorption and yield of cotton

doi:10.6048/j.issn.1001-4330.2024.04.004

Abstract

Abstract:

【Objective】 To investigate the effects of urease inhibitor N-butyl thiophosphoryl triamine (NBPT) and nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) on nutrient uptake and yield in cotton. 【Methods】 Using field experiments, the tested crops were cotton Xinluzao 64 and Lumianyan 24. Four treatments were set up: no nitrogen fertilizer (N₀), nitrogen fertilizer (N₃₀₀), nitrogen fertilizer+urease inhibitor (N₃₀₀+NBPT), and nitrogen fertilizer+nitrification inhibitor (N₃₀₀+DMPP). 【Results】 The results showed that compared to N₀, all nitrogen application treatments significantly increased cotton biomass and promoted the absorption of nutrient nitrogen, phosphorus, and potassium by cotton plants. The order of nitrogen fertilizer utilization efficiency from high to low is N₃₀₀+DMPP>N₃₀₀+NBPT>N₃₀₀. Applying nitrogen fertilizer and urease/nitrification inhibitors can significantly increase cotton yield. The Lumianyan 24 had better nutrient absorption, nitrogen utilization efficiency, and higher yield than Xinluzao 64, but its biomass was significantly lower than that of Xinluzao 64. 【Conclusion】 Adding urease and nitrification inhibitors can promote the absorption and utilization of nutrients by cotton plants, improve nitrogen fertilizer utilization efficiency, and ultimately effectively increase cotton yield.

Key words: cotton; urease inhibitors; nitrification inhibitors; nutrient absorption; yield

摘要：

【目的】研究添加脲酶抑制剂N-丁基硫代磷酰三胺(NBPT)和硝化抑制剂3,4-二甲基吡唑磷酸盐(DMPP)对棉花养分吸收和产量的影响。【方法】采取大田试验,供试棉花品种为新陆早64号和鲁棉研24号,共设置4个处理:不施氮肥(N₀)、施氮肥(N₃₀₀)、氮肥+脲酶抑制剂(N₃₀₀+NBPT)、氮肥+硝化抑制剂(N₃₀₀+DMPP)。【结果】与N₀相比,所有施氮处理均显著增加棉花生物量,促进棉花植株对养分氮、磷和钾的吸收。氮肥利用率从高到低顺序为N₃₀₀+DMPP>N₃₀₀+NBPT>N₃₀₀。施加氮肥和脲酶/硝化抑制剂均能显著提高棉花产量。鲁棉研24号养分吸收、氮肥利用率以及产量均优于新陆早64号,但生物量却显著低于新陆早64号。【结论】添加脲酶抑制剂和硝化抑制剂可以促进棉花植株对养分的吸收利用,并提高氮肥利用率和棉花产量。

关键词: 棉花, 脲酶抑制剂, 硝化抑制剂, 养分吸收, 产量

CLC Number:

S562

YE Yang, HOU Zhen'an, MIN Wei, GUO Huijuan. Effects of urease/nitrification inhibitors on nutrient absorption and yield of cotton[J]. Xinjiang Agricultural Sciences, 2024, 61(4): 814-822.

叶扬, 侯振安, 闵伟, 郭慧娟. 添加脲酶/硝化抑制剂对棉花养分吸收和产量的影响[J]. 新疆农业科学, 2024, 61(4): 814-822.

Figures/Tables 6

Fig.1 Changes of different treatments on cotton stem, leaf, bud and total biomass Note:(a), (b), (c), and (d) respectively represent the effects of different treatments on cotton stems, leaves, buds, and total biomass. The value labeled with similar letters indicate significant difference among treatments (P<0.05)

Fig.2 Changes of different treatments on cotton N absorption Note: (a), (b), (c) and (d) show the effects of different treatments on cotton stems, leaves, buds and bolls and total N uptake. Different letters in the figure indicate that the difference between treatments reaches a significant level (P<0.05)

Fig.3 Changes of different treatments on cotton P absorption Note: (a), (b), (c) and (d) show the effects of different treatments on cotton stems, leaves, buds and bolls and total P uptake. Different letters in the figure indicate that the difference between treatments reaches a significant level (P<0.05)

Fig.4 Changes of different treatments on cotton K absorption Note:Figures (a), (b), (c) and (d) show the effects of different treatments on cotton stems, leaves, buds and bolls and total K uptake. Different letters in the figure indicate that the difference between treatments reaches a significant level (P<0.05)

Fig.5 Changes of different treatments on cotton yield Note:Different letters in the figure indicate that the difference between treatments reaches a significant level (P<0.05)

Tab.1 Changes of Nitrogen fertilizer utilization efficiency of cotton plants under different treatments

品种 Breed	处理 Treatments	氮肥利用率 Nitrogen use efficiency (%)
新陆早64号 Xinluzao 64	N₀	—
	N₃₀₀	43.40
	N₃₀₀+NBPT	49.69
	N₃₀₀+DMPP	64.64
鲁棉研24号 Lumianyan 24	N₀	—
	N₃₀₀	46.29
	N₃₀₀+NBPT	56.481
	N₃₀₀+DMPP	68.94

References 35

[1]	张海清. 中国棉花产区比较优势研究[D]. 北京: 中国农业科学院, 2004.
	ZHANG Haiqing. A Study on Comparative Advantage in Cotton-growing Regions of China[D]. Beijing: Chinese Academy of Agricultural Sciences, 2004.
[2]	刘迪荣. 对新疆棉花品种布局的建议[J]. 中国棉花, 2001, 28(9): 26.
	LIU Dirong. Suggestions on the layout of cotton varieties in Xinjiang[J]. China Cotton, 2001, 28(9): 26.
[3]	中华人民共和国统计局. 中国统计年鉴[J]. 北京: 中国统计出版社, 2022.
	Statistics Bureau of the People's Republic of China. China Statistical Yearbook[J]. Beijing: China Statistical Publishing House, 2022.
[4]	廖育林, 荣湘民, 刘强, 等. 氮肥施用量与莴苣产量和品质及氮肥利用率的关系[J]. 湖南农业大学学报(自然科学版), 2005, 31(1): 47-49.
	LIAO Yulin, RONG Xiangmin, LIU Qiang, et al. Effects of nitrogen rates on yield and quality and nitrogen use efficiency of Asparagus lettuce[J]. Journal of Hunan Agricultural University (Natural Sciences), 2005, 31(1): 47-49.
[5]	朱兆良, 金继运. 保障我国粮食安全的肥料问题[J]. 植物营养与肥料学报, 2013, 19(2): 259-273.
	ZHU Zhaoliang, JIN Jiyun. Fertilizer use and food security in China[J]. Plant Nutrition and Fertilizer Science, 2013, 19(2): 259-273.
[6]	Chen X X, Wang C Y, Fu J J, et al. Synthesis, inhibitory activity and inhibitory mechanism studies of Schiff base Cu(II) complex as the fourth type urease inhibitors[J]. Inorganic Chemistry Communications, 2019, 99: 70-76.
[7]	Fu J J, Wang C Y, Chen X X, et al. Classification research and types of slow controlled release fertilizers (SRFs) used-a review[J]. Communications in Soil Science and Plant Analysis, 2018, 49(17): 2219-2230.
[8]	赵亚南, 宿敏敏, 吕阳, 等. 减量施肥下小麦产量、肥料利用率和土壤养分平衡[J]. 植物营养与肥料学报, 2017, 23(4): 864-873.
	ZHAO Yanan, SU Minmin, LYU Yang, et al. Wheat yield, nutrient use efficiencies and soil nutrient balance under reduced fertilizer rate[J]. Journal of Plant Nutrition and Fertilizer, 2017, 23(4): 864-873.
[9]	王趁义, 陈仙仙, 黄兆玮, 等. 第四类脲酶抑制剂对土壤脲酶活性和微生物量的影响[J]. 水土保持通报, 2019, 39(2): 149-154.
	WANG Chenyi, CHEN Xianxian, HUANG Zhaowei, et al. Effects of fourth-type urease inhibitors on soil urease activity and microbial growth[J]. Bulletin of Soil and Water Conservation, 2019, 39(2): 149-154.
[10]	周旋, 吴良欢, 戴锋. 生化抑制剂组合对黄泥田土壤尿素态氮转化的影响[J]. 水土保持学报, 2015, 29(5): 95-100, 123.
	ZHOU Xuan, WU Lianghuan, DAI Feng. Effects of combined biochemical inhibitors on transformation of urea-N in yellow clayey soil[J]. Journal of Soil and Water Conservation, 2015, 29(5): 95-100, 123.
[11]	Trenkel M E. Characteristics and types of slow-and controlled-release fertilizers and nutrification and urease inhibitors[A]. Paris: International Fertilizer Industry Association (IFA), 2010: 56.
[12]	Singh S N, Verma A. Environmental review: the potential of nitrification inhibitors to manage the pollution effect of nitrogen fertilizers in agricultural and other soils: a review[J]. Environmental Practice, 2007, 9(4): 266-279.
[13]	周旋, 吴良欢, 戴锋. 新型磷酰胺类脲酶抑制剂对不同质地土壤尿素转化的影响[J]. 应用生态学报, 2016, 27(12): 4003-4012. DOI
	ZHOU Xuan, WU Lianghuan, DAI Feng. Influence of a new phosphoramide urease inhibitor on urea-N transformation in different texture soil[J]. Chinese Journal of Applied Ecology, 2016, 27(12): 4003-4012. DOI
[14]	王雪薇, 刘涛, 褚贵新. 三种硝化抑制剂抑制土壤硝化作用比较及用量研究[J]. 植物营养与肥料学报, 2017, 23(1): 54-61.
	WANG Xuewei, LIU Tao, CHU Guixin. Inhibition of DCD, DMPP and Nitrapyrin on soil nitrification and their appropriate use dosage[J]. Journal of Plant Nutrition and Fertilizer, 2017, 23(1): 54-61.
[15]	王静, 王允青, 张凤芝, 等. 脲酶/硝化抑制剂对沿淮平原水稻产量、氮肥利用率及稻田氮素的影响[J]. 水土保持学报, 2019, 33(5): 211-216.
	WANG Jing, WANG Yunqing, ZHANG Fengzhi, et al. Effects of urease/nitrification inhibitors on yield and nitrogen utilization efficiency of rice and soil nitrogen of paddy field in plain along the Huaihe River[J]. Journal of Soil and Water Conservation, 2019, 33(5): 211-216.
[16]	鲁艳红, 聂军, 廖育林, 等. 氮素抑制剂对双季稻产量、氮素利用效率及土壤氮平衡的影响[J]. 植物营养与肥料学报, 2018, 24(1): 95-104.
	LU Yanhong, NIE Jun, LIAO Yulin, et al. Effects of urease and nitrification inhibitor on yield, nitrogen efficiency and soil nitrogen balance under double-rice cropping system[J]. Journal of Plant Nutrition and Fertilizers, 2018, 24(1): 95-104.
[17]	鲍士旦. 土壤农化分析[M]. 北京: 中国农业出版社, 1981.
	BAO Shidan. Agrochemical analysis of soil[M]. Beijing: China Agriculture Press, 1981.
[18]	王静, 王允青, 万水霞, 等. 脲酶/硝化抑制剂对沿淮平原糯稻养分吸收利用的影响[J]. 江苏农业学报, 2020, 36(1): 77-82.
	WANG Jing, WANG Yunqing, WAN Shuixia, et al. Effects of urease/nitrification inhibitor on nutrient uptake of glutinous rice in plain along the Huaihe River[J]. Jiangsu Journal of Agricultural Sciences, 2020, 36(1): 77-82.
[19]	周旋, 吴良欢, 戴锋. 生化抑制剂组合与施肥模式对黄泥田水稻养分累积及利用率的影响[J]. 中国生态农业学报, 2017, 25(10): 1495-1507.
	ZHOU Xuan, WU Lianghuan, DAI Feng. Effects of combined biochemical inhibitors and fertilization models on nutrient uptake and use efficiency of rice in yellow clayey field[J]. Chinese Journal of Eco-Agriculture, 2017, 25(10): 1495-1507.
[20]	王小彬, L·D·Bailey, C·A·Grallt, 等. 关于几种土壤脲酶抑制剂的作用条件[J]. 植物营养与肥料学报, 1998, 4(3): 211-218.
	WANG Xiaobin, L·D·BAILEY, C·A·GRALLT, et al. The acting conditions of some urease inhibitors in soils[J]. Plant Natrition and Fertilizen Science, 1998, 4(3): 211-218.
[21]	孙爱文, 石元亮, 张德生, 等. 硝化/脲酶抑制剂在农业中的应用[J]. 土壤通报, 2004, 35(3): 357-361.
	SUN Aiwen, SHI Yuanliang, ZHANG Desheng, et al. Application of nitrification-urease inhibitors in agriculture[J]. Chinese Journal of Soil Science, 2004, 35(3): 357-361.
[22]	姚云柯, 徐卫红, 周豪, 等. 脲酶/硝化抑制剂缓释肥对番茄养分吸收和土壤肥力的影响[J]. 西南农业学报, 2018, 31(4): 748-753.
	YAO Yunke, XU Weihong, ZHOU Hao, et al. Effect of special slow release fertilizer containing urease/nitrification inhibitor on nutrient uptake of tomato and soil fertility[J]. Southwest China Journal of Agricultural Sciences, 2018, 31(4): 748-753.
[23]	Dawar K, Rahman U, Alam S S, et al. Nitrification inhibitor and plant growth regulators improve wheat yield and nitrogen use efficiency[J]. Journal of Plant Growth Regulation, 2022, 41(1): 216-226.
[24]	Zhang M, Fan C H, Li Q L, et al. A 2-yr field assessment of the effects of chemical and biological nitrification inhibitors on nitrous oxide emissions and nitrogen use efficiency in an intensively managed vegetable cropping system[J]. Agriculture, Ecosystems & Environment, 2015, 201: 43-50.
[25]	胡娟, 吴景贵, 孙继梅, 等. 氮肥减量与缓控肥配施对土壤供氮特征及玉米产量的影响[J]. 水土保持学报, 2015, 29(4): 116-120, 194.
	HU Juan, WU Jinggui, SUN Jimei, et al. Effects of reduced nitrogen fertilization and its combined application with slow and controlled release fertilizers on soil nitrogen characteristics and yield of maize[J]. Journal of Soil and Water Conservation, 2015, 29(4): 116-120, 194.
[26]	米国华, 伍大利, 陈延玲, 等. 东北玉米化肥减施增效技术途径探讨[J]. 中国农业科学, 2018, 51(14): 2758-2770. DOI
	MI Guohua, WU Dali, CHEN Yanling, et al. The ways to reduce chemical fertilizer input and increase fertilizer use efficiency in maize in Northeast China[J]. Scientia Agricultura Sinica, 2018, 51(14): 2758-2770. DOI
[27]	阚建鸾, 王晓云, 苏建平, 等. 不同氮肥抑制剂对小麦产量、土壤肥力、氮肥利用率的影响[J]. 中国农学通报, 2023, 39(5): 69-74. DOI
	KAN Jianluan, WANG Xiaoyun, SU Jianping, et al. Effects of different nitrogen fertilizer inhibitors on wheat yield, soil fertility and nitrogen use efficiency[J]. Chinese Agricultural Science Bulletin, 2023, 39(5): 69-74. DOI
[28]	Liu C, Wang K, Zheng X. Effects of nitrification inhibitors (DCD and DMPP) on nitrous oxide emission, crop yield and nitrogen uptake in a wheat-maize cropping system[J]. Biogeosciences, 2013, 10(4): 2427-2437.
[29]	Cui L, Li D P, Wu Z J, et al. Effects of combined nitrification inhibitors on nitrogen transformation, maize yield and nitrogen uptake in two different soils[J]. Communications in Soil Science and Plant Analysis, 2022, 53(8): 1039-1049.
[30]	Byrnes B H, Freney J R. Recent developments on the use of urease inhibitors in the tropics[J]. Fertilizer Research, 1995, 42(1): 251-259.
[31]	Wilson C E Jr, Wells B R, Norman R J. Dicyandiamide influence on uptake of preplant-applied fertilizer nitrogen by rice[J]. Soil Science Society of America Journal, 1990, 54(4): 1157-1161.
[32]	Turner D A, Edis R B, Chen D, et al. Determination and mitigation of ammonia loss from urea applied to winter wheat with N-(n-butyl) thiophosphorictriamide[J]. Agriculture, Ecosystems & Environment, 2010, 137(3/4): 261-266.
[33]	盛素君, 王趁义, 朱培飞, 等. 脲酶抑制剂在水稻增产中的应用研究进展[J]. 安徽农业科学, 2012, 40(21): 10762, 10928.
	SHENG Sujun, WANG Chenyi, ZHU Peifei, et al. Research progress in the application of urease inhibitor to the yield increase of rice[J]. Journal of Anhui Agricultural Sciences, 2012, 40(21): 10762, 10928.
[34]	张文, 刘铨义, 曾庆涛, 等. 不同行距配置对机采棉生长发育及光合特性的影响[J]. 干旱地区农业研究, 2022, 40(5): 155-164.
	ZHANG Wen, LIU Quanyi, ZENG Qingtao, et al. Effects of different row spacing on the growth and photosynthetic characteristics of machine picked cotton[J]. Agricultural Research in the Arid Areas, 2022, 40(5): 155-164.
[35]	魏鑫, 徐建辉, 张巨松. 种植模式对机采棉干物质积累及品质的影响[J]. 新疆农业科学, 2017, 54(7): 1177-1184.
	WEI Xin, XU Jianhui, ZHANG Jusong. Effects of planting patterns on dry matter accumulation and quality of machine-harvesting cotton[J]. Xinjiang Agricultural Sciences, 2017, 54(7): 1177-1184.