Effects of different priming treatments on seed vigor and physiological characteristics of spring wheat

doi:10.6048/j.issn.1001-4330.2024.04.010

Abstract

Abstract:

【Objective】 To study the effects of different seed priming on seed germination and seedling growth characteristics of spring wheat in the hope of providing theoretical basis for improving seed vitality of the wheat.【Methods】 Xinchun 26 wheat seeds were taken as the test material, and primed with different concentrations of SA (0.05, 0.1, 0.15 mmol/L), CaCl₂ (6, 9, 12 mmol/L) and water for different time 4, 6, 8, 10, 12 and 14 h, respectively to measure the indexes such as the germination potential, germination rate, germination index, vigor index, fresh dry weight and length of seedling and root, electrical conductivity, SOD activity, POD activity, dehydrogenase activity and MDA content and other factors.【Results】 The results showed that the seed germination potential, germination rate, germination index, vigor index, fresh dry weight, seedling length and root length, SOD, POD, dehydrogenase activity and other indexes of spring wheat treated with different initiators were significantly increased compared with the control, and the electrical conductivity and malondialdehyde content were significantly decreased, among which A₂ and B₁ treatments had the best effect. Under the condition of different priming time, germination index, seedling growth index and physiological index of each initiator treatment reached the maximum value at 12 h, and there was significant difference between them and other priming time.【Conclusion】 Seed priming enhances the enzyme activity of spring wheat seeds, effectively resists membrane peroxidation, reduces the content of membrane peroxidation products, maintains the integrity of cell structure, and effectively improves the seed activity of spring wheat, and promotes the growth of seedlings. Among them, 0.1 mmol/L SA and 6 mmol/L CaCl₂ have the best effect on the priming time of 12 h.

Key words: spring wheat; seed priming; seed vigor; physiological characters

摘要：

【目的】研究引发剂对春小麦种子萌发及幼苗生长特性的影响,为增强春小麦种子活力提供理论依据。【方法】以春小麦新春26号种子为供试材料,用不同浓度水杨酸SA(0.05、0.1、0.15 mmol/L)、氯化钙CaCl₂(6、9、12 mmol/L)、蒸馏水H₂O分别引发4、6、8、10、12和14 h后回干,测定春小麦种子发芽势、发芽率、发芽指数、活力指数、幼苗鲜干重、苗长和根长、电导率、SOD活性、POD活性、脱氢酶活性和丙二醛含量等指标。【结果】不同引发剂处理春小麦种子发芽势、发芽率、发芽指数、活力指数、幼苗鲜干重、苗长和根长、SOD、POD、脱氢酶活性等指标均较对照显著提高,电导率和丙二醛含量显著降低,其中A₂处理和B₁处理的效果最优。引发12 h时各引发剂处理的春小麦发芽指标、幼苗生长指标、生理指标均达到最大值,与其他引发时间之间差异显著。【结论】引发剂处理使春小麦种子酶活性增强,有效地抵抗了膜质过氧化作用,减少膜质过氧化产物含量,维持细胞结构的完整性,从而有效提高春小麦种子活力,促进幼苗生长,其中0.1 mmol/L SA和6 mmol/L CaCl₂引发12 h的效果最佳。

关键词: 春小麦, 种子引发, 种子活力, 生理特性

CLC Number:

S512

Gulinigaer Tuerhong, ZHANG Jinshan, LI Dandan, ZHANG Lulu, WANG Runqi, SHI Shubing. Effects of different priming treatments on seed vigor and physiological characteristics of spring wheat[J]. Xinjiang Agricultural Sciences, 2024, 61(4): 869-877.

古力尼尕尔·吐尔洪, 张金汕, 李丹丹, 张路路, 王润琪, 石书兵. 不同引发剂处理对春小麦种子活力及生理特性的影响[J]. 新疆农业科学, 2024, 61(4): 869-877.

Figures/Tables 6

References 38

[1]	丁忆然, 李卫华, 柴亚茹, 等. 人工老化和修复处理对小麦种子活力及生理特性的影响[J]. 种子, 2016, 35(4): 13-16, 21.
	DING Yiran, LI Weihua, CHAI Yaru, et al. Effects of artificial accelerated aging and repairing process on seed vigor and physiological characteristics of wheat seeds[J]. Seed, 2016, 35(4): 13-16, 21.
[2]	李如雪. 小麦种子活力状况分析与种子活力评价技术研究[D]. 泰安: 山东农业大学, 2020.
	LI Ruxue. Analysis of Seed Vigor Status and Study on the Evaluation Technology of Seed Vigor in Wheat[D]. Taian: Shandong Agricultural University, 2020.
[3]	马晔, 杨进文, 李宁, 等. 双氧水与PEG协同引发对小麦种子活力的影响[J]. 农学学报, 2022, 12(4): 6-12. DOI
	MA Ye, YANG Jinwen, LI Ning, et al. Synergistic initiation effect of hydrogen peroxide and PEG on wheat seed vigor[J]. Journal of Agriculture, 2022, 12(4): 6-12. DOI
[4]	阮松林, 薛庆中. 植物的种子引发[J]. 植物生理学通讯, 2002,(2):198-202.
	RUAN Songlin, XUE Qingzhong. Plant seed priming[J]. Plant Physiology Communications, 2002,(2):198-202.
[5]	史启申. 种子引发及贮藏对番茄砧木种子发芽、幼苗生长和耐冷性的影响[D]. 郑州: 河南农业大学, 2019.
	SHI Qishen. The Effects of Seed Priming and Storage on Seed Germination, seedling Growth and Cold Tolerance of Tomato Rootstocks[D]. Zhengzhou: Henan Agricultural University, 2019.
[6]	尹玲莉, 侯晓杰. 植物抗性信号分子——水杨酸研究进展[J]. 中国农学通报, 2007, 23(1): 338-342.
	YIN Lingli, HOU Xiaojie. The recent advances of salicylic acid as signal molecules of resistance in plant[J]. Chinese Agricultural Science Bulletin, 2007, 23(1): 338-342. DOI
[7]	雷慧霞. 种质库保存及CaCl₂等4种引发剂处理对白鲜、芍药种子活力的影响[D]. 北京: 中国农业科学院, 2019.
	LEI Huixia. Effects of Germplasm Bank Storage and CaCl₂ Four Initiators Treatments on Seed Vigor of Dictamnus Dasycarpus Turcz.Seeds and Paeonia Lactiflora Pall.Seeds[D]. Beijing: Chinese Academy of Agricultural Sciences, 2019.
[8]	王彦荣, 张建全, 刘慧霞, 等. PEG引发紫花苜蓿和沙打旺种子的生理生态效应[J]. 生态学报, 2004, 24(3): 402-408.
	WANG Yanrong, ZHANG Jianquan, LIU Huixia, et al. Physiological and ecological responses of alfalfa and milkvetch seed to PEG priming[J]. Acta Ecologica Sinica, 2004, 24(3): 402-408.
[9]	朱文彬, 王长林. 种子引发对盐碱地夏枯草出苗质量的影响[J]. 江苏农业科学, 2017, 45(4): 106-108.
	ZHU Wenbin, WANG Changlin. Effect of seed initiation on the emergence quality of Prunella vulgaris in saline-alkali land[J]. Jiangsu Agricultural Sciences, 2017, 45(4): 106-108.
[10]	张彦萍, 刘海河, 申书兴, 等. 多胺引发处理对茄子种子活力及幼苗耐冷性的影响[J]. 园艺学报, 2010, 37(11): 1783-1788.
	ZHANG Yanping, LIU Haihe, SHEN Shuxing, et al. Effect of polyamine priming on seed vigor and seedling chilling tolerance in eggplant[J]. Acta Horticulturae Sinica, 2010, 37(11): 1783-1788.
[11]	马超, 孔蓓蓓, 张均, 等. 不同引发剂处理对水分胁迫下小麦发芽及幼苗生理特性的影响[J]. 核农学报, 2017, 31(2): 357-363. DOI
	MA Chao, KONG Beibei, ZHANG Jun, et al. Effects of seed priming with different agents on seed germination and seedling physiological characteristics of wheat under dehydration stress[J]. Journal of Nuclear Agricultural Sciences, 2017, 31(2): 357-363. DOI
[12]	毛培胜, 张晔, 宋玉梅, 等. 褪黑素引发对盐胁迫敖汉苜蓿种子发芽特性的影响[J]. 种子, 2019, 38(6): 36-42.
	MAO Peisheng, ZHANG Ye, SONG Yumei, et al. Effects of melatonin priming on seeds germination characteristics in Aohan alfalfa under saline stress[J]. Seed, 2019, 38(6): 36-42.
[13]	逯亚玲, 王灵婧, 王宁, 等. 外源水杨酸对NaCl胁迫下紫花苜蓿幼苗生长和生理特性的影响[J]. 草地学报, 2017, 25(6): 1265-1273. DOI
	LU Yaling, WANG Lingjing, WANG Ning, et al. Effects of salicylic acid on physiological characteristics and growth of alfalfa seedling under NaCl stress[J]. Acta Agrestia Sinica, 2017, 25(6): 1265-1273. DOI
[14]	黄玉梅, 张杨雪, 刘庆林, 等. 水杨酸对盐胁迫下百日草种子萌发及幼苗生理特性的影响[J]. 草业学报, 2015, 24(7): 97-105. DOI
	HUANG Yumei, ZHANG Yangxue, LIU Qinglin, et al. Effects of salicylic acid on seed germination and seedling physiological characteristics of Zinnia elegans under salt stress[J]. Acta Prataculturae Sinica, 2015, 24(7): 97-105. DOI
[15]	吴道藩. 提高甘蓝(Brassica oleracea l.var.capitata)种子活力的方法与机理研究[D]. 重庆: 西南农业大学, 2002.
	WU Daofan. Study on Methods and Mechanisms of Improving Vigor in Cabbage (Brassica Oleracea L.var.capitata) Seed[D]. Chongqing: Southwest Agricultural University, 2002.
[16]	孙妙, 杨周婷, 张存莉, 等. 中国沙棘种子的水引发技术及其抗性生理效应[J]. 林业科学, 2014, 50(12): 32-39.
	SUN Miao, YANG Zhouting, ZHANG Cunli, et al. Hydro-priming technique and its resistance physiology effect for sea buckthorn seed[J]. Scientia Silvae Sinicae, 2014, 50(12): 32-39.
[17]	张凤. 低温贮藏对不同含水量种子活力的影响[D]. 泰安: 山东农业大学, 2014.
	ZHANG Feng. Effect of Low Temperature on Seed Vigor with Different Moisture Contents[D]. Taian: Shandong Agricultural University, 2014.
[18]	王玉利. 小麦储藏生理变质相关表征指标研究及其应用[D]. 郑州: 河南工业大学, 2016.
	WANG Yuli. The Characteristics Index of Physiological Deterioration of Stored Wheat and Its Application[D]. Zhengzhou: Henan University of Technology, 2016.
[19]	Paparella S, Araújo S S, Rossi G, et al. Seed priming: state of the art and new perspectives[J]. Plant Cell Reports, 2015, 34(8): 1281-1293. DOI PMID
[20]	刘建新, 刘瑞瑞, 贾海燕, 等. NaHS引发提高裸燕麦种子活力的生理机制[J]. 草业学报, 2021, 30(2): 135-142. DOI
	LIU Jianxin, LIU Ruirui, JIA Haiyan, et al. Physiological mechanism of NaHS priming improvement of seed vigor in naked oat[J]. Acta Prataculturae Sinica, 2021, 30(2): 135-142. DOI
[21]	周天美. 甘蓝种子引发处理的生物学效应及引发处理后的适宜贮藏温度[D]. 南京: 南京农业大学, 2016.
	ZHOU Tianmei. The Preparation and Immune Protective Efficacy of Novel Bivalent Vaccines of Porcine Reproductive and Respiratory Syndrome Virus and Porcine Circovirus Type 2[D]. Nanjing: Nanjing Agricultural University, 2016.
[22]	王铁兵, 王鹏, 蒋建军, 等. 不同药剂引发处理对老化玉米种子萌发及幼苗生长的影响[J]. 中国草地学报, 2020, 42(5): 31-39.
	WANG Tiebing, WANG Peng, JIANG Jianjun, et al. Effects of different initiators on germination and seedlings growth of aged maize seeds[J]. Chinese Journal of Grassland, 2020, 42(5): 31-39.
[23]	马金虎, 郭数进, 王玉国, 等. 种子引发对盐胁迫下高粱幼苗生物量分配和渗透物质含量的影响[J]. 生态学杂志, 2010, 29(10): 1950-1956.
	MA Jinhu, GUO Shujin, WANG Yuguo, et al. Effects of seed priming on biomass allocation and osmotic substance contents of Sorghum (Sorghum bicolor L.) seedlings under salt stress[J]. Chinese Journal of Ecology, 2010, 29(10): 1950-1956.
[24]	贺长征, 胡晋, 朱志玉, 等. 混合盐引发对水稻种子在逆境条件下发芽及幼苗生理特性的影响[J]. 浙江大学学报(农业与生命科学版), 2002, 28(2): 1950-1956.
	HE Changzheng, HU Jin, ZHU Zhiyu, et al. Effect of seed priming with mixed-salt solution on germination and physiological characteristics of seedling in rice (Oryza sativa L.) under stress conditions[J]. Journal of Zhejiang Agricultural University (Agric & Life Sci), 2002, 28(2): 1950-1956.
[25]	马文广, 崔华威, 李永平, 等. 不同药剂引发处理对干旱胁迫下烟草种子发芽和幼苗生长的影响[J]. 浙江农业学报, 2012, 24(6): 949-956.
	MA Wenguang, CUI Huawei, LI Yongping, et al. Effects of seed priming with different agents on seed germination and seedling growth in tobacco(Nicotiana tabacum L.) under drought stress[J]. Acta Agriculturae Zhejiangensis, 2012, 24(6): 949-956.
[26]	de Aquino G S, Di Loreto Y Sampaio M, Lobak T, et al. Chemical and physical treatments can change the germination and seed vigor of Crambe (Crambe abyssinica Hochst). A case study[J]. Australian Journal of Crop Science, 2018, 12(12): 1844-1848.
[27]	陈德碧, 朱建勇. 水杨酸对番茄种子萌发及幼苗生长铬胁迫的缓解效应[J]. 北方园艺, 2010,(2): 13-16.
	CHEN Debi, ZHU Jianyong. Mitigative effect of salicylic acid on tomato seed germination and seedling growth under chromium stress[J]. Northern Horticulture, 2010,(2): 13-16.
[28]	王鹏, 韦月平. 水杨酸浸种对蚕豆种子萌发的影响[J]. 吉林特产高等专科学校学报, 2004, 13(3):4-6.
	WANG Peng, WEI Yueping. Effect of salicylic acid soaking on seed germination of broad[J]. Journal of Jilin Specialty College, 2004, 13(3):4-6.
[29]	牟筱玲, 胡晓丽. 水杨酸浸种对棉花种子萌发及幼芽的影响[J]. 中国棉花, 2003, 30(12): 19-21.
	MOU Xiaoling, HU Xiaoli. Effect of salicylic acid soaking on cotton seed germination and bud[J]. China Cotton, 2003, 30(12): 19-21.
[30]	王英, 何立荣. 水杨酸浸种对小麦种子萌发的影响[J]. 安徽农业科学, 2017, 45(11): 39-40.
	WANG Ying, HE Lirong. Effect of seed soaking with salicylic acid on seed germination of wheat[J]. Journal of Anhui Agricultural Sciences, 2017, 45(11): 39-40.
[31]	穆瑞霞. 人工老化与引发对大葱种子活力及幼苗生理生化变化的影响[D]. 郑州: 河南农业大学, 2008.
	MU Ruixia. Effects of Artificial Aging and Seed Priming on Seed Vigor and Seedlings’ Physiological and Biochemical Changes of Welsh Onion[D]. Zhengzhou: Henan Agricultural University, 2008.
[32]	王建华, 高扬帆, 吴艳兵, 等. 氯化钙和青霉素对黄瓜老化种子发芽及幼苗生长的影响[J]. 河南科技学院学报(自然科学版), 2008, 36(2): 35-36.
	WANG Jianhua, GAO Yangfan, WU Yanbing, et al. Effect of CaCl₂ and penicillin on germination of cucumber aged seed and the growing of seeding[J]. Journal of Henan Institute of Science and Technology (Natural Science Edition), 2008, 36(2): 35-36.
[33]	刘晶, 张鹤婷, 殷悦, 等. 外源硫化氢对干旱胁迫下萌发水稻种子抗氧化代谢的影响[J]. 南方农业学报, 2017, 48(1): 31-37.
	LIU Jing, ZHANG Heting, YIN Yue, et al. Effects of exogenous hydrogen sulfide on antioxidant metabolism of rice seed germinated under drought stress[J]. Journal of Southern Agriculture, 2017, 48(1): 31-37.
[34]	何立荣, 王英. 水杨酸浸种对小麦种子萌发生理生化指标的影响[J]. 黑龙江农业科学, 2017, (2): 37-39.
	HE Lirong, WANG Ying. Effect of salicylic acid soaking on the physiological biochemistry targets of wheat seed germination[J]. Heilongjiang Agricultural Sciences, 2017, (2): 37-39.
[35]	高巧红, 丁希政, 牛志浩, 等. 水杨酸引发处理对低温吸胀期间玉米种子生理指标的影响[J]. 河南农业科学, 2017, 46(3): 41-46.
	GAO Qiaohong, DING Xizheng, NIU Zhihao, et al. Effect of salicylic acid priming on physiological indexes of maize seed during seed imbibition under low temperature[J]. Journal of Henan Agricultural Sciences, 2017, 46(3): 41-46.
[36]	邢燕, 王吉庆, 菅广宇, 等. 不同引发剂处理对西瓜种子萌发及生理特性的影响[J]. 中国农学通报, 2009, 25(11): 133-136.
	XING Yan, WANG Jiqing, JIAN Guangyu, et al. Effect of different materials priming on the germination and physiological characteristics of aged watermelon seed[J]. Chinese Agricultural Science Bulletin, 2009, 25(11): 133-136. DOI
[37]	施春才, 冯国惠. 玉米浸种催芽断根技术应用试验[J]. 现代化农业, 2015, (11): 14-15.
	SHI Chuncai, FENG Guohui. Application test of maize seed soaking to accelerate germination and root cutting technology[J]. Modernizing Agriculture, 2015, (11): 14-15.
[38]	李秀梅, 古吉, 李亚清, 等. 不同引发温度及时间对烟草种子低温萌发的影响[J]. 种子, 2020, 39(5): 99-103.
	LI Xiumei, GU Ji, LI Yaqing, et al. Effects of different initiation temperature and time on low temperature germination of tobacco seeds[J]. Seed, 2020, 39(5): 99-103.

处理 Treatments	发芽势 Germination Potential(%)	发芽率 Germination Rate(%)	发芽指数 Germination index	活力指数 Vigor index
CK	69.33±3.06^d	83.33±1.15^d	51.68±2.60^d	0.6957±0.06^d
H	79.33±4.16^c	90.67±3.06^c	57.41±2.01^c	0.8619±0.04^c
A₁	89.33± 3.06^ab	95.33±4.16^abc	64.33±1.66^a	0.9822±0.04^ab
A₂	93.33±3.06^a	96.67±1.15^a	66.25±2.55^a	1.0727±0.08^a
A₃	87.33±4.16^ab	94.00±2.00^abc	62.34±2.51^ab	0.9752±0.07^abc
B₁	91.33±2.31^ab	96.00±2.00^ab	65.75±4.27^a	1.0524±0.08^a
B₂	86.00±2.00^b	91.33±2.31^bc	59.35±2.52^bc	0.9003±0.05^bc
B₃	88.00±4.00^ab	93.33±3.06^abc	61.88±1.52^abc	0.9597±0.06^abc

处理 Treatments	发芽势 Germination Potential(%)	发芽率 Germination Rate(%)	发芽指数 Germination index	活力指数 Vigor index
CK	69.33±3.06^d	83.33±1.15^d	51.68±2.60^d	0.6957±0.06^d
H	79.33±4.16^c	90.67±3.06^c	57.41±2.01^c	0.8619±0.04^c
A₁	89.33± 3.06^ab	95.33±4.16^abc	64.33±1.66^a	0.9822±0.04^ab
A₂	93.33±3.06^a	96.67±1.15^a	66.25±2.55^a	1.0727±0.08^a
A₃	87.33±4.16^ab	94.00±2.00^abc	62.34±2.51^ab	0.9752±0.07^abc
B₁	91.33±2.31^ab	96.00±2.00^ab	65.75±4.27^a	1.0524±0.08^a
B₂	86.00±2.00^b	91.33±2.31^bc	59.35±2.52^bc	0.9003±0.05^bc
B₃	88.00±4.00^ab	93.33±3.06^abc	61.88±1.52^abc	0.9597±0.06^abc

指标 Factors	处理 Treatments	引发时间Priming time(h)
指标 Factors	处理 Treatments	4	6	8	10	12	14
苗鲜重 Seedling fresh weight(g)	CK	0.103 5^a	0.103 5^a	0.103 5^a	0.103 5^a	0.103 5^a	0.103 5^a
	H	0.109 3^ab	0.106 2^bc	0.106 8^bc	0.105 5^bc	0.114 7^a	0.103 1^c
	A	0.112 6^b	0.106 9^b	0.107 9^b	0.107 2^b	0.133 4^a	0.110 73^b
	B	0.115 4^b	0.108 8^bc	0.111 0^bc	0.108 6^bc	0.126 0^a	0.110 4^bc
苗干重 Seedling dry weight(g)	CK	0.013 5^a	0.013 5^a	0.013 5^a	0.013 5^a	0.013 5^a	0.013 5^a
	H	0.014 0^bc	0.013 4^c	0.013 9^bc	0.014 1^b	0.015 0^a	0.013 6^bc
	A	0.014 7^b	0.014 0^bc	0.014 6^b	0.014 1^bc	0.015 7^a	0.014 0^bc
	B	0.014 7^b	0.014 2^b	0.014 7^b	0.014 3^b	0.015 6^a	0.014 3^b
根鲜重 Root fresh weight(g)	CK	0.170 9^a	0.170 9^a	0.170 9^a	0.170 9^a	0.170 9^a	0.170 9^a
	H	0.184 6^ab	0.178 2^bc	0.176 8^bc	0.177 3^bc	0.189 5^a	0.176 3^bc
	A	0.186 8^b	0.185 6^b	0.182 0^bc	0.179 9^bc	0.204 4^a	0.180 6^bc
	B	0.190 8^a	0.189 3^a	0.184 1^ab	0.185 7^ab	0.199 7^a	0.187 4^a
根干重 Root dry weight(g)	CK	0.010 4^a	0.010 4^a	0.010 4^a	0.010 4^a	0.010 4^a	0.010 4^a
	H	0.012 5^ab	0.011 1^cd	0.011 9^abc	0.011 1^cd	0.012 9^a	0.011 3^bcd
	A	0.012 4^b	0.011 2^d	0.012 2^bc	0.011 8^bcd	0.014 2^a	0.011 4^cd
	B	0.013 1^a	0.011 6^b	0.012 4^ab	0.011 9^b	0.013 5^a	0.011 5^b
苗长 Seedling length(cm)	CK	14.413 3^a	14.413 3^a	14.413 3^a	14.413 3^a	14.413 3^a	14.413 3^a
	H	15.553 3^ab	14.843 3^cd	15.050 0^bc	15.103 3^bc	15.766 7^a	15.186 7^bc
	A	15.803 3^ab	15.166 7^bc	15.026 7^cd	15.390 0^bc	16.320 0^a	15.286 7^bc
	B	15.926 7^ab	15.333 3^b	15.486 7^b	15.630 0^ab	16.130 0^a	15.350 0 ^b
根长 Root length (cm)	CK	13.946 7^a	13.946 7^a	13.946 7^a	13.946 7^a	13.946 7^a	13.946 7^a
	H	15.240 0^b	14.023 3^d	14.826 7^bc	14.233^cd	16.036 7^a	14.433 3^cd
	A	15.590 3^b	14.743 0^c	15.557 8^b	15.193 9^bc	17.215 3^a	14.713 0^c
	B	15.792 8^b	15.262 2^bc	15.672 8^b	15.008 8^bc	17.062 3^a	14.766 2^c

指标 Factors	处理 Treatments	引发时间Priming time(h)
指标 Factors	处理 Treatments	4	6	8	10	12	14
苗鲜重 Seedling fresh weight(g)	CK	0.103 5^a	0.103 5^a	0.103 5^a	0.103 5^a	0.103 5^a	0.103 5^a
	H	0.109 3^ab	0.106 2^bc	0.106 8^bc	0.105 5^bc	0.114 7^a	0.103 1^c
	A	0.112 6^b	0.106 9^b	0.107 9^b	0.107 2^b	0.133 4^a	0.110 73^b
	B	0.115 4^b	0.108 8^bc	0.111 0^bc	0.108 6^bc	0.126 0^a	0.110 4^bc
苗干重 Seedling dry weight(g)	CK	0.013 5^a	0.013 5^a	0.013 5^a	0.013 5^a	0.013 5^a	0.013 5^a
	H	0.014 0^bc	0.013 4^c	0.013 9^bc	0.014 1^b	0.015 0^a	0.013 6^bc
	A	0.014 7^b	0.014 0^bc	0.014 6^b	0.014 1^bc	0.015 7^a	0.014 0^bc
	B	0.014 7^b	0.014 2^b	0.014 7^b	0.014 3^b	0.015 6^a	0.014 3^b
根鲜重 Root fresh weight(g)	CK	0.170 9^a	0.170 9^a	0.170 9^a	0.170 9^a	0.170 9^a	0.170 9^a
	H	0.184 6^ab	0.178 2^bc	0.176 8^bc	0.177 3^bc	0.189 5^a	0.176 3^bc
	A	0.186 8^b	0.185 6^b	0.182 0^bc	0.179 9^bc	0.204 4^a	0.180 6^bc
	B	0.190 8^a	0.189 3^a	0.184 1^ab	0.185 7^ab	0.199 7^a	0.187 4^a
根干重 Root dry weight(g)	CK	0.010 4^a	0.010 4^a	0.010 4^a	0.010 4^a	0.010 4^a	0.010 4^a
	H	0.012 5^ab	0.011 1^cd	0.011 9^abc	0.011 1^cd	0.012 9^a	0.011 3^bcd
	A	0.012 4^b	0.011 2^d	0.012 2^bc	0.011 8^bcd	0.014 2^a	0.011 4^cd
	B	0.013 1^a	0.011 6^b	0.012 4^ab	0.011 9^b	0.013 5^a	0.011 5^b
苗长 Seedling length(cm)	CK	14.413 3^a	14.413 3^a	14.413 3^a	14.413 3^a	14.413 3^a	14.413 3^a
	H	15.553 3^ab	14.843 3^cd	15.050 0^bc	15.103 3^bc	15.766 7^a	15.186 7^bc
	A	15.803 3^ab	15.166 7^bc	15.026 7^cd	15.390 0^bc	16.320 0^a	15.286 7^bc
	B	15.926 7^ab	15.333 3^b	15.486 7^b	15.630 0^ab	16.130 0^a	15.350 0 ^b
根长 Root length (cm)	CK	13.946 7^a	13.946 7^a	13.946 7^a	13.946 7^a	13.946 7^a	13.946 7^a
	H	15.240 0^b	14.023 3^d	14.826 7^bc	14.233^cd	16.036 7^a	14.433 3^cd
	A	15.590 3^b	14.743 0^c	15.557 8^b	15.193 9^bc	17.215 3^a	14.713 0^c
	B	15.792 8^b	15.262 2^bc	15.672 8^b	15.008 8^bc	17.062 3^a	14.766 2^c

处理 Treatments	超氧化物歧化酶活性 Superoxide dismutase activity (SOD,U/g)	过氧化物酶活性 Peroxidase activity (POD,U/(min·g))	脱氢酶OD值 Dehydrogenase	丙二醛含量 Malondialdehyde content (μmol/g)
CK	209.10 ^f	34.50^e	0.115^g	5.33^a
H	231.56^e	42.17^d	0.138^f	4.05^b
A₁	264.31^c	48.50^b	0.125^e	3.97^b
A₂	304.19^a	56.08^a	0.163^a	2.88^c
A₃	247.96^d	43.50^cd	0.142^c	4.01^b
B₁	293.46^b	54.08^a	0.162^a	3.18^bc
B₂	244.89^d	43.75^cd	0.134^d	4.25^b
B₃	258.69^c	46.42^bc	0.153^b	3.70^bc