Effects of salt stress on ion balance, membrane lipid peroxidation, and osmotic regulation substance accumulation in thin skin muskmelon ‘Huishu’

doi:10.6048/j.issn.1001-4330.2024.04.014

Xinjiang Agricultural Sciences ›› 2024, Vol. 61 ›› Issue (4): 900-907.DOI: 10.6048/j.issn.1001-4330.2024.04.014

• Agricultural Product Analysis and Detection·Soil Fertilizer·Agricultural Product Processing Engineering • Previous Articles Next Articles

Effects of salt stress on ion balance, membrane lipid peroxidation, and osmotic regulation substance accumulation in thin skin muskmelon ‘Huishu’

YANG Wenli¹(), XU Lirong², LIU Bin¹, LING Yueming¹, LI Meihua¹, YANG Yong¹, FAN Rong¹, LI Yushun¹, ZHANG Yongbin¹, ZHANG Xuejun¹()

1. Hami Melon Research Center,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China
2. Horticulture College, Xinjiang Agricultural University, Urumqi 830091,China

Received:2023-08-01 Online:2024-04-20 Published:2024-05-31
Correspondence author: ZHANG Xuejun
Supported by:
China Agriculture Research System of watermelon and melon(CARS-25);Open Project of Xinjiang Key Laboratory of Crop Biotechnology(XJYS0302-2022-4);Special Project for Key Cultivation of Scientific and Technological Innovation of Xinjiang Academy of Agricultural Sciences(xjkcpy-2022006);Xinjiang Uygur Autonomous Region Tianchi Talent Project

盐胁迫对薄皮甜瓜‘灰鼠’离子平衡、膜脂过氧化及渗透调节物质积累的影响

杨文莉¹(), 许丽蓉², 刘斌¹, 凌悦铭¹, 李寐华¹, 杨永¹, 范蓉¹, 黎玉顺¹, 张永兵¹, 张学军¹()

1.新疆农业科学院哈密瓜研究中心,乌鲁木齐 830091
2.新疆农业大学园艺学院,乌鲁木齐 830091

通讯作者: 张学军
作者简介:杨文莉(1994-),女,河南人,助理研究员,研究方向为西甜瓜抗逆育种,(E-mail)1481209164@qq.com
基金资助:
国家西甜瓜产业技术体系-特色甜瓜品种改良岗(CARS-25);新疆农作物生物技术重点实验室开放课题(XJYS0302-2022-4);新疆农业科学院科技创新重点培育专项(xjkcpy-2022006);新疆维吾尔自治区天池英才项目

Abstract

Abstract:

【Objective】 To explore the physiological response mechanism of thin-skinned melon ‘Huishu’ to salt (NaCl) stress and provide theoretical basis for melon cultivation, demonstration and promotion. 【Methods】 In this study, a substrate pot experiment was conducted to observe the changes in seedling growth, ion homeostasis, antioxidant enzyme system, and osmoregulation substances after 100 mmol/L NaCl short-term stress (5 d) and long-term stress (20 d).【Results】 Salt stress inhibited seedling growth, destroyed ion balance and antioxidant systems. The plant height, petiole length, and leaf number decreased significantly with the prolongation of salt stress time, while the diameter, petiole diameter, and leaf thickness increased significantly with the prolongation of salt stress time. After salt stress, the Na⁺ efflux velocity decreased, the Na⁺ content increased, the K⁺ outflow velocity increased, and the K⁺ content decreased. The content of malondialdehyde increased significantly. The content of malondialdehyde significantly increased, and the activities of SOD, POD, and CAT enzymes first increased and then decreased; the content of soluble sugar, soluble protein, and free proline increased significantly. 【Conclusion】 Under salt stress, the thin-skinned melon ‘Huishu’ responds to salt stress by limiting its own growth, increasing the Na⁺ content and reducing the K⁺ content of its leaves, improving the antioxidant enzyme system, and accumulating more osmoregulation substances.

Key words: melon; NMT technology; ion equilibrium; antioxidant system; osmoregulation

摘要：

【目的】研究薄皮甜瓜‘灰鼠’对盐(NaCl)胁迫的生理响应机制,为甜瓜栽培及示范推广提供理论依据。【方法】采用基质盆栽试验,分别观测其在100 mmol/L NaCl胁迫5 d及胁迫20 d后在幼苗生长、离子稳态、抗氧化酶系统及渗透调节物质的变化情况。【结果】盐胁迫抑制了幼苗生长,破坏了离子平衡及抗氧化系统。甜瓜株高、叶柄长、叶片数随盐胁迫时间的延长而显著降低,茎粗、叶柄粗、叶片厚度随盐胁迫时间的延长而显著增加。盐胁迫后Na⁺外排速度降低,Na⁺含量增加,K⁺外流流速增大,K⁺含量减少。丙二醛含量显著增加,SOD、POD、CAT酶活性先升高后降低;可溶性糖、可溶性蛋白、游离脯氨酸含量显著增加。【结论】盐胁迫下,薄皮甜瓜‘灰鼠’通过限制自身生长,增加叶片Na⁺含量、减少K⁺含量,并提高抗氧化酶活性,积累更多的渗透调节物质缓解盐胁迫。

关键词: 甜瓜, NMT技术, 离子平衡, 抗氧化系统, 渗透调节

CLC Number:

S652

YANG Wenli, XU Lirong, LIU Bin, LING Yueming, LI Meihua, YANG Yong, FAN Rong, LI Yushun, ZHANG Yongbin, ZHANG Xuejun. Effects of salt stress on ion balance, membrane lipid peroxidation, and osmotic regulation substance accumulation in thin skin muskmelon ‘Huishu’[J]. Xinjiang Agricultural Sciences, 2024, 61(4): 900-907.

杨文莉, 许丽蓉, 刘斌, 凌悦铭, 李寐华, 杨永, 范蓉, 黎玉顺, 张永兵, 张学军. 盐胁迫对薄皮甜瓜‘灰鼠’离子平衡、膜脂过氧化及渗透调节物质积累的影响[J]. 新疆农业科学, 2024, 61(4): 900-907.

Figures/Tables 6

Tab.1 Changes of ‘Huishu’ growth indexes under salt stress

处理时间 Treatment Time (d)	株高 Plant height (cm)	茎粗 Diameter diameter (mm)	叶片长 Leaf long (cm)	叶片宽 Leaf side (cm)	叶片厚度 Blade thickness (mm)	叶柄长 Petiole length (mm)	叶柄粗 Petiole thick (mm)	叶片数 Leaf number
0	64.83±3.25^a	3.98±0.41^a	9.83±0.76^a	12.43±0.43^a	0.35±0.03^a	10±1.68^a	3.36±0.1^a	8±1^a
5	54.3±5.5^b	4.15±0.5^a	7.5±0.58^b	9.83±0.76^b	0.44±0.02^b	8.7±1.89^a	3.36±0.06^a	6±0^b
20	40±8.54^c	4.46±0.32^a	6.5±0.71^b	9.25±1.06^b	0.55±0.06^c	7±2.65^a	3.43±0.02^a	5±1^b

Fig.1 Changes of average K+、Na+ fluxes in seedling leaves of ‘Huishu’ after 100 mmol/L NaCl stress for 24 hours

Fig.2 Changes of salt stress on K+ and Na+ contents in leaves of ‘Huishu’seedlings thin muskmelon

Fig.3 Changes of salt stress on malondialdehyde content in leaves of thin muxkmelon‘Huishu’ seedlings

Fig.4 Changes of salt stress on antioxidant enzyme activities in leaves of ‘Huishu’ seedlings

Fig.5 Changes of salt stress on osmoregulation substances in leaves of ‘Huishu’ seedlings Note:A. B and C are the contents of soluble sugar, soluble protein and free proline, respectively

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Effects of salt stress on ion balance, membrane lipid peroxidation, and osmotic regulation substance accumulation in thin skin muskmelon ‘Huishu’

盐胁迫对薄皮甜瓜‘灰鼠’离子平衡、膜脂过氧化及渗透调节物质积累的影响

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