Ultrastructural observations of leg sensilla in Eupeodes corollae using scanning electron microscopy

doi:10.6048/j.issn.1001-4330.2024.04.018

Abstract

Abstract:

【Objective】 The objective of this study is to elucidate the types, features, and distribution of leg sensilla in female adult Eupeodes corollae, in the hope of providing a morphological foundation for further studying on the egg-laying selection mechanism of the natural enemy E. corollae. 【Methods】 Using scanning electron microscopy, the ultrastructure of leg sensilla in female E. corollae adults was observed. 【Results】 The results showed that the legs of female adults of E. corollae consisted of six segments, including coxa, trochanter, femur, tibia, tarsus, and pretarsus. In total, five types of sensilla were distributed on the legs: sensilla basiconica (subtypes I, II, and III), sensilla trichodea (long sensilla trichodea subtypes I and II; short sensilla trichodea subtypes I and II), sensilla chaetica (subtypes I and II), Böhm bristles, and microtrichiae. Sensilla basiconica (SB) were exclusively located on the tarsus, and one subtype SB I had a pore at its tip, suggesting a crucial function in chemoreception. Sensilla trichodea and sensilla chaetica were distributed on the femur, tibia, and tarsus of legs in female adult. These two types of sensilla had the longitudinal furrows on their hairy surface, no wall pore, and a movable socket at the base, which were characteristic features of mechanosensilla. The number of Böhm bristles and microtrichiae were large and widely distributed around various types of sensilla. 【Conclusion】 In this study, five types of sensilla are identified on the leg of female E. corollae. The abundance and diverse types of sensilla are found in the tarsus segments of the legs, denoting their critical role in chemosensory perception.

Key words: Eupeodes corollae; tarsus; sensilla basiconica; ultrastructure; chemosensation; mechanosensation

摘要：

【目的】分析大灰优蚜蝇Eupeodes corollae雌成虫胸足的感器类型、特征及分布,为天敌昆虫食蚜蝇产卵选择机制奠定形态学基础。【方法】利用扫描电镜技术观察其足上感器的超微结构。【结果】大灰优蚜蝇雌成虫胸足由基节、转节、腿节、胫节、跗节和前跗节6个部分组成,其上分布5种类型的感器,分别为锥形感器(3种亚型I~III)、毛形感器(长毛形感器2种亚型I~II;短毛形感器2种亚型I~II)、刺形感器(2种亚型I~II)、Böhm氏鬃毛和微毛感器。锥形感器仅分布于足跗节,其中锥形感器I亚型顶端具孔,在化学感受过程中发挥重要的作用;毛形感器和刺形感器在雌成虫胸足腿节、胫节和跗节均有分布,这2种类型感器的毛干表面有纵脊,无壁孔,基部具可活动的臼状窝,是典型的机械感器;Böhm 氏鬃毛和微毛感器数量较多,散布在各类感器之间。【结论】在大灰优蚜蝇雌成虫胸足上鉴定了5种类型的感器,足跗节上的感器类型和数量最多,是足上重要的化学感受区域。

关键词: 大灰优蚜蝇, 足跗节, 锥形感器, 超微结构, 化学感受, 机械感受

CLC Number:

Q964
S43

WU Jinan, DONG Wanying, LIU Tongxian, WANG Bing, WANG Guirong. Ultrastructural observations of leg sensilla in Eupeodes corollae using scanning electron microscopy[J]. Xinjiang Agricultural Sciences, 2024, 61(4): 937-944.

吴基楠, 董婉莹, 刘同先, 王冰, 王桂荣. 大灰优蚜蝇雌成虫足感器扫描电镜分析[J]. 新疆农业科学, 2024, 61(4): 937-944.

Figures/Tables 4

Fig.1 Scanning electron micrographs of foreleg in female adults of Eupeodes corollae Note: A. General view of the part of leg from Eupeodes corollae, showing the femur, the tibia, the tarsus, the pretarsus; B. General view of the femur; C. General view of the tibia; D. General view of the tarsus; E. General view of the pretarsus (yellow rectangular border); Femur (Fe); Tibia (Ti); Tarsus (Tar); Pretarsus (Pretar)

Fig.2 Scanning electron micrographs of femur and tibia in foreleg of female adults of E. corollae Note: A. Sensilla trichodea in the femur; B. Long sensilla trichodea I (LST I) and short sensilla trichodea I (SST I); C. Böhm bristles (BB), epidermal fossa at the base of sensilla trichodea (ST); D. Sensilla chaetica (SC) and böhm bristles (BB) at the tibia

Fig.3 Scanning electron micrographs of tarsus in foreleg of female adults of E. corollae Note: A. Short sensilla chaetica (SSC) and long sensilla chaetica (LSC) at the back of the tarsus; B. Short sensilla chaetica (SSC) and long sensilla chaetica (LSC) at the both sides of the tarsus; C. Short sensilla chaetica (SSC) and böhm bristles (BB) at the tarsus; D. Microtrichiae (Mt); E. Long sensilla trichodea Ⅱ (LST Ⅱ) and short sensilla trichodea Ⅱ (SST Ⅱ) at the ventral sides of the tarsus; E. SB I, SB II and SB III at the ventral sides of the tarsus; G. SB I; H. SB II; I. SB III

Fig.4 Scanning electron micrographs of pretarsus in foreleg of female adults of E. corollae Note: A. General view of ventral view of the pretarsus (yellow rectangular border); B. General view of back view of the pretarsus (yellow rectangular border); C. Claw and seta at the back side of the pretarsus; D. Puvillus at the ventral side of the pretarsus

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