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WAN Peng-cheng;SHI Wen-yan;DAI Rong;ZHOU Ping;SHI Guo-qing;ZENG Shen-ming
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万鹏程;石文艳;代蓉;周平;石国庆;曾申明
摘要: [目的]探讨胚胎附植调控因子αV,β3整合素在绵羊早期体外胚胎的表达情况,为早期胚胎发育及附植调控机理提供理论依据.[方法]采用RT-PCR和间接免疫荧光染色技术,检测绵羊体外受精个发育阶段胚胎中αV及β3整合素mRNA的表达模式及蛋白表达定位情况.[结果]在绵羊2-细胞至桑椹胚体外胚胎中未能检测到整合素αV及β3 mRNA的表达,但可以检测到囊胚期胚胎有微量整合素αV及β3 mRNA的表达.并且在绵羊第7d扩张囊胚和第8d孵化囊胚上均可检测到整合素αVβ3蛋白的表达,且蛋白表达主要分布在胚胎滋养层细胞周围,孵化囊胚蛋白表达量略高于扩张囊胚.[结论]αV,β3整合素在绵羊早期胚胎发育及附植过程中具有重要调控作用.
WAN Peng-cheng;SHI Wen-yan;DAI Rong;ZHOU Ping;SHI Guo-qing;ZENG Shen-ming. The Expression Pattern of αV, β3 Integrin on Ovine Embryo Derived From in Vitro Fertilization[J]. .
万鹏程;石文艳;代蓉;周平;石国庆;曾申明. αV,β3整合素在绵羊体外受精胚胎的表达模式研究[J]. .
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http://www.xjnykx.com/EN/Y2014/V51/I9/1711