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    25 January 2010, Volume 47 Issue 1 Previous Issue    Next Issue

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    Study on NHX1 Gene Functional Comparisons between Chenopodium glaucum and Oriza sativa Using Affymetrix Gene Chips in Transgenic Arabidopsis
    GUO Ji-na;ZHANG Fu-chun
    2010, 47(1): 1-6. 
    Abstract ( )  
    [Objective and Method]The gene chip is a DNA analysis technology of high throughput and high efficiency which has been widely used in analysis of resistant function.In this study, using Affymetrix Arabidopsis whole-genome expression gene chip, the differential expression analysis of gene was carried out with the transgenic A. thaliana .[Result]In the transgenic Arabidopsis tissues under salt stress, 394 co-expression genes were selected from the four groups, they accounted for 1.64; of the total number of genes, in which there were 177 up-regulated expression genes and 217 down-regulated expression genes. These genes were initially divided into 12 gene categories, including response to abiotic or biotic stimuli, response to stress , electron transport or energy pathways, signal transduction and transcription, and so on. Our results show that the process of plant salt-tolerant is a multi-gene changed complex process that involves many changes of variety related genes.[Conclusion]The results of comparison scatters and cluster analysis illuminate that the salt tolerance of transgenic Arabidopsis cgNHX1, cgNHX1dc, osNHX1 and osNHX1dc have successively weakened.Our study will contribute to find the differences between halophytic and glycophytic species in the mechanism of plant salt tolerance and the function analysis of hydrophilic C-terminal, provide a favorable evidence for the study of the mechanism of plant salt-tolerance between halophytic and glycophytic species, and offer new methodology in study the mechanism for salt-tolerant plants, provide a basis for practical applications of salt-tolerant mechanism in plants.
    Analysis of the Ecological Bearing Capacity in Kanas Scenic Spot Based on "3S" Technology
    ZHENG Wei;ZHU Jin-zhong;PAN Cun-de
    2010, 47(1): 7-13. 
    Abstract ( )  
    [Objective]The ecological bearing capacity is the quantitative evaluation index for sustainable development.[Method]In order to make the dynamic analysis of ecological bearing capacity in Kanas Scenic Spot, the authors obtained information on the land use and natural landscape change in 1992, 2000, 2005 in Kanas Scenic Spot based on "3S". The evaluation indices system of ecological bearing capacity in Kanas Scenic Spot was established using principal components analysis (PCA) and analytic hierarchy process (AHP). On this basis, 13 indices including the resources, environment and social-economy, which were separated from the resource bearing capacity, environment bearing capacity, ecological elasticity and ecological bearing pressure, were chosen as the sustainable development degrees indices. And the whole Kanas Scenic Spot(or different period) was divided into several sustainable development degrees with different sustainability of ecosystems according to the ecological load-bearing (negative direction and positive direction) index calculated. [Result]The results showed the ecological bearing index in Kanas Scenic Spot was decreased from 0.066 9 in 1992,to 0.048 5 in 2000 and 0.004 6 in 2005.The sustainable bearing state was decreased from sustainal bearing capacity in 1992 and 2000 to the poor one in 2005.[Conclusion]Which was occurred mainly due to the increasing of tourism development, population and economical activities. Thereby,all the obtained different data provided scientific basis for the regional sustainable development.
    Somatic Embryogenesis and Plant Regeneration in Cotton Cultivars from Planting Areas of Xinjiang and Yellow River Cotton
    YAN Yong-liang;QU Yan-ying;DAI Xin;WANG Xiang-yong;YANG Zhen;CHEN Quan-jia
    2010, 47(1): 14-19. 
    Abstract ( )  
    [Objective and Method]In this study 6 genotypes of upland cotton were selected for comparison of their regenerability,including 2 cultivars from Yellow River valley, 3 cultivars from Xinjiang,using a highly responsible genotypes Coker 312 as a material. [Result]Most of the genotypes showed high regenerability under the IBA/KT hormone regime.[Conclusion]Under the optimized culture procedure, most of the genotypes collected from the two planting areas showed the similar regeneable abilities.The total number of embryos and frequency of embryo germination of "XLZ15"was higher for cotton transformation. This study will improve the transgenic efficiency in Xinjiang.
    Construction of Cotton GhCAD6 Expression Vector and Transient Expression in Onion by Detecting GUS
    NI Zhi-yong;L(U) Meng;WANG Juan;LI Bo;BAI Yan;FAN Ling
    2010, 47(1): 20-24. 
    Abstract ( )  
    [Objective and Method]In this study β-glucuronidase gene (GUS) was used as reporter gene in biolistic transformation experiment. Transient expression vector of cotton GhCAD6 gene was constructed. [Result]Firstly, the GhCAD6 gene cDNA was obtained from pGEM-T-CAD6 by PCR, then it was cloned into transient expression vector pRTL2-GUS/NIa. The transient expression vector pGUS-CAD6 is driven by CaMV35S promoter with GUS reporter gene and the target gene, GUS reporter gene and target gene simultaneously express in single cell. Secondly, verified by digestion with restriction enzymes, the vector pGUS-CAD6 was transformed into onion using PDS-1000/He biolistic particle delivery system. The GUS gene expression was detected in 24 h after bombardment,strong GUS gene expression was observed in the epidermal cells of onion under microscope, the results indicated that the transient expression vector pGUS-CAD6 could be highly effectively expressed in plant cell. [Conclusion]The results of this paper provide a strong foundation for analyzing function of GhCAD6 gene in cotton in the future.
    Chemical Treatment of Slow-release Mepiquat Chloride from Coated Cotton Seed in South Xinjiang
    ZHAO Qiang;ZHANG Ju-song;TIAN Xiao-li;PENG Xiao-feng;LI Bing;Aimaitijiang;ZHOU Chun-jiang
    2010, 47(1): 25-30. 
    Abstract ( )  
    [Objective and Method]Chemical treatment of slow-release mepiquat chloride from cotton coating seed, which has practical significance in saving cost and increasing benefit, is one way of simplified cultivation of cotton. This paper analyzed the effect of slow-release mepiquat chloride by using different doses of mepiquat chloride in coating seed. It involved in the influence of slow-release mepiquat chloride on cotton growth, agronomic and economic characters, and so on. [Mesult]The results showed that slow-release mepiquat chloride of middle and high dose had good effects on avoiding spindling of cotton before topping, and its function was similar to foliar application of mepiquat chloride in regulating the growth and agronomic characters of cotton.Slow-release mepiquat chloride has little effect on yield. [Conclusion]Chemical manipulation of slow-release mepiquat chloride can replace the foliar application of mepiquat chloride before topping.
    Establishment of Double Direction Gel Electrophoresis of Cotton Fibre Protein
    WANG Juan;NI Zhi-yong;SHI Qin-hua;L(U) Meng;LI Bo;BAI Yan;FAN Ling
    2010, 47(1): 31-35. 
    Abstract ( )  
    [Objective and Method]The objective of this paper was to compare with three different protein extraction methods of Gossypium hirsutum, cv. Xuzhou 142 and the effects of various lysis buffers on thedouble direction electrophoresis (2-D). The loading quantity of sample and staining method were also optimized for the 2-D analysis.[Result]The results showed that the protein content of cotton fibre extracted by saturated powder was highter than that extraced by other methods and the electrophoresis strip of SDS-PAGE were clear;the solubility raised whose protein were dissolved in the mixture of thiourea and CHAPS; the more suitable loading quantity was 300 μg to the pH 3-10 7 cm IPG strip. Furthermore, the protein spots were increased and the background was clear by the modified Coomassie Brilliant Blue method.[Conclusion]The double direction Gel Electrophoresis method used to extract cotton fibre protein was initially established.
    A Study on Relation of Together Growth of Organs and Cotton Bolls Spatial Distribution of Super High-yielding Cotton
    ZHANG Xin-guo;CHEN Qian;ZHANG Ju-song;CHEN Guan-wen;SONG Ji-hui
    2010, 47(1): 36-41. 
    Abstract ( )  
    [Objective and Method]The aim of this text was mainly to study relations of together growth on organs and cotton bolls'spatial distribution of Super high-yielding cotton,[Result]the relation of together growth of fruit branches'first cotton bud and the main stem leaves is n+1.5~n-1.2;The relation of together growth of fruit branches'first flower and the main stem leaves is n+6.2~n+8.8(n for the bit of main stem leaf with the same frui-bit). [Conclusion]The proportion of the central-lower and interior part of bolls of super high-yielding conventional varieties cotton reached 90;,it is the main part of super high-yielding cotton bolls, The top part of bolls is about 10;,the top and external bolls is ultra-high yield potential; The proportion of the central bolls of super high-yielding hybrid'cotton is 50;, the proportion of the lower and top bolls is almost same, the bolls of foliage branch is 4.3;, The proportion of the external bolls is nearly 40;, the top and external bolls is the key of super high-yielding cotton.
    Analysis on Heterosis of Yield and Traits of Self-cultivated Intervarietal Crossing of Sea Island Cotton
    DING Sheng;LU Nai-zeng;YANG Dong-jie;ZHANG Li-li;MEI Yong-jun
    2010, 47(1): 42-46. 
    Abstract ( )  
    [Objective and Method]Five yield traits of 6 parents and their 15 F_1 crosses (6×6) of Sea island cotton were analyzed by the genetic model of additive-dominance effect by MINQUE(1) approaches. [Result]The results indicated that there existed highly significant additive effect on boll number, lint percentage and lint yield per plant (except for boll weight), highly significant dominance effect on boll number, boll weight and lint percentage (except for lint yield per plant ). [Conclusion]There was a significant positive super-parant heterosis in the average genetic performance of boll number and lint percentage, but there was no positive population advantage in the yield of boll weight and lint percentage per plant.
    Effect of Various Amount of Fertilizer Planting and Densities on Yield and Sugar Content of New Sweet Sorghum 3
    JIA Dong-hai;WANG Zhao-mu;LIN Ping;CHEN Yue-hua
    2010, 47(1): 47-53. 
    Abstract ( )  
    [Objective and Method]Best amount of densities and fertilizer can build excellent group structure and is the bases of group building under fine light effect, so this paper studies the yield and sugar content of stem in New Sweet Sorghum 3 after carrying out different densities and amount of fertilizer base on two factors and five level trial by using the group.The main purpose of this article was designed to study the effects of various amount of fertilizer and planting densities on the yield and sugar content of New Sweet Sorghum 3.[Result]The results showed that the plant fresh biomass,the yield of stem,grain,stem sugar content of New Sweet Sorghum 3 have varied significantly under the treatments of different densities and fertilizer in Ili area of north Xinjiang.All the fresh biomass yield,stem yield and sugar yield were lower from D1 to D5 under high planting density ; however,there were no significant difference under fertilizations; there were significant difference in seed yield under planting density and fertilization; also there were no difference in sugar content under planting density and fertilization. [Conclusion]It was suggested that the planting density of 166 650 plants per hectare and fertilization 450kg per hectare was appropriate for New Sweet Sorghum 3.
    Analysis of Combining Ability and Applied Evaluation of Early Maturing Maize Inbred Lines
    SHAO Hong-yu;HAN Deng-xu;Abulaiti·Abula;LIANG Xiao-ling;LI Ming-dong
    2010, 47(1): 54-58. 
    Abstract ( )  
    [Objective and Method]The early maturing maize inbred lines have been tested in this paper with the designing Zign of P1×P2 incomplete diallel crossing of NCⅡ method to study combining ability of 11 traits.Ana1ysis of the general combining ability showed that 11 traits was significantly difference.The special combining ability of 11 traits had significant difference.[Result]The result indicated that the lines of Wu314,Xinzi349,Xinzi3113-1 and ZPA82/9 had higher general combining ability (GCA) of yield per plant., the lines of Xinzi3112,Xinzi3119 and Xinzi4202 must be modified before application,the line of Xinzi3113-1 can be directly used but the traits of plant height and ear seat of it must be controlled by plant height and ear height.There were great effect value of negative combining ability of bare tip of ZPA82/9,Wu314 and Xinzi349 .The line of Zao314L had great negative effect value of combining ability and plant height.[Conclusion]The combinations of Xinzi3113-1×OS87-24,Xinzi3112×L02,Xinzi3119×Zao314L,Xinzi3112×Wu314,Xinzi4202×Xinzi349 and Xinzi4202×ZPA82/9 had special combining ability(SCA) of high yield per plant.
    Study on Physiology of Drought Resistance of Atriplex canescens
    LEI Jun-jie;NIE Xin-hui;GAO Wen-wei;Sailihan·Sai;GAO Yong-hong
    2010, 47(1): 59-66. 
    Abstract ( )  
    [Objective and Method]The objectives of this study were to reveal the ten plants′ content of proline, TTC, MDA, soluble sugar, soluble protein, SOD, POD before and after stress treatments and were to compare their changes and analyze the physiological indexes of Atriplex canescens and other drought-resistant plants quatitatively and qualitatively.[Result]The results showed the content of proline of Atriplex canescens was the highest compared with that of other 9 kinds of plants.The plant in content of MDA was the lowest before and after stress treatments.The content of soluble sugar and soluble protein was in second before and after stress treatment. [Conclusion]It can clearly be seen,Atriplex canescens had the strongest drought-resistance compared with that of other plants.
    QTL Mapping of Yield and Agronomic Traits of Interspecific Hybrid Cotton
    SHI Li-fang;HU Lei;HU Bao-min;CHEN Ling;WANG Pei-zheng
    2010, 47(1): 67-72. 
    Abstract ( )  
    [Objective and Method]In the present paper, a genetic linkage map was constructed using 148 F_2 individuals developed by cross of an upland cotton TM-1 and a sea-island cotton Hai7124. 97 SSR markers were chosen which showed stable polymorphism between two parents. This genetic linkage map covered a total of recombinational length of 673.3 cM with the average interval distance of 17.3 cM. Six agronomic traits including the first fruit node, node number, boll number, boll weight, lint percentage and leaf area were investigated. [Result]Three possible QTLs/loci with LOD≥2.5 were suggested. One QTL of lint percentage was identified on linkage group 4; one QTL of node number was located on linkage group 1; one QTL of leaf area was located on linkage group 6. [Conclusion]These QTLs had greater effect value which can be used effectively for MAS during the process of breeding in the future.
    Establishment of Callus Regeneration System of English Lavender
    LIU Shan;CHEN Quan-jia;SU Xiu-juan;WANG Li-ping;QU Yan-ying;ZHOU Meng-ying
    2010, 47(1): 73-77. 
    Abstract ( )  
    [Objective and Metod]In this experiment,the callus regeneration system of Lavender was established by the use of it as meterial,which layed the basis for fast reproduction of Lavender and genetic transformation.[Result]It was shown that the induction results of the MS +2,4-D 0.10 mg/L + KT0. 30 mg/L medium groups were better than that of other groups.The yellowish callus from 2-3 generations were continuously cultivated on the medium of MS+6-BA 2.0 mg/L+NAA 0.50 mg/L,which form great number of thickly-growing root,when young bud reached 1-2 cm,then was put in medium with MS+6-BA 1.0 mg/L to be expanded for 1-2 generations.After that,the young bud was put in medium with 1/2 MS+IAA 1.0 mg/L to continue to be cultiveated to be rooting.[Conclusion]In 20 days,the rooting rate reached 33;.
    Correlation of Early Maturing Traits and QTL Mapping in Cotton
    LI Ke;LI Zhi-bo;WEI Yi-nong
    2010, 47(1): 78-81. 
    Abstract ( )  
    [Objective and Method]The correlation betewwn growth periods(the days of budding, flowering, opening boll) and agricultural traits(plant height and first fruiting branch node) were analyzed in a F_2 population, which was derived from a cross between Ghirsutum L."Jin13" and Gbarbadense L. "Hai7124". And QTLs detection of these traits were employed in this population. [Result]The conclusion showed that the day of opening boll was positively correlated with the day of budding, flowering, boll setting, but negatively correlated with plant height, and no significant correlation with first fruiting branch node. A total of 18 QTLs were detected by composite interval mapping, including 4 QTLs for opening boll on chromosome 2, 22, 25 and D3. The variances explained by each QTL from 69.6; to 89.9;. The QTLs for budding to opening boll and flwering to opening boll were detected in the same area of chromosome 2, 25 and D3. 2 QTLs for budding were separately detected on chromosome 9 and 17. 2 QTLs for flowering were separately detected on chromosome 15 and 26, and 2 QTLs for bud setting were detected in same area of these chromosomes. The QTL for plant height and first fruiting branch node were detected in same area of chromosome A3.[Conclusion]These QTLs have provided evidence for early maturing cotton breeding .
    Effect of Different Temperature on Quality and Physiological Index of Postharvest Red Globe Grape during Storage
    Wuernisha·Kader;CHE Feng-bin;ZHANG Ting;LI Ping;HU Bai-wen
    2010, 47(1): 82-86. 
    Abstract ( )  
    [Objective and Method]The study was conducted to determine the five different temperatures for Red Globe grape storage experiment and reveal the quality and physiological index of postharvest Red Globe grape,such as the changes of respiration、the production rate of C_2H_2,firmness,soluble solids content,cell membrane permeability,MDA content under the temperature of 1℃,0℃,-1℃,-2℃,-3℃.[Result and Conclusion]The result showed that -1℃ is the best storage temperature for the Red Globe grape,in this temperature,the change tendency of respiration and the production rate of C_2H_2 is gentle,the fruit was kept in suitable firmness.It was slightly better than that of other temperature,MDA content was lower and soluble solids content was higher than other processing.
    Research on Storage Effect of Preservative Solution on Aksu Jujub Quality
    ZHANG Ting;CHE Feng-bin;Aiermaike;HU Bai-wen;LI Ping;PAN Yan
    2010, 47(1): 87-91. 
    Abstract ( )  
    [Objective and Method]Aksu Jujub fruits were treated with A,B,chitosan,1-MCP,2,4-D before it was placed in plastic film bags and stored at -1℃.[Result]The results showed that this treatment of A+2,4-D could delay the decrease of Aksu Jujub fruit firmness ,titrable acidity content and total sugar content,postpone the degradation of Vitamin C in fruit,slow down the increase of relative electrical conductivity,keep the fruit to be lower change of red fruit rate during the storage,keep the quality of the Aksu Jujub better,delay its senescence.[Conclusion]This treatment of chitosan + B could keep the lower fragility rate and fruit rotten,and rotten rate fruit rate was only 0.19; after 108 days's storge,control the disease of the postharvest Aksu Jujub during storage.
    Research on Improving Water Use Efficency of Populus euphratica under Orthogonal Test
    WU Wei;LI Jian-gui
    2010, 47(1): 92-97. 
    Abstract ( )  
    [Objective and Method]In the Populus euphratica original ecological environment,the 4-factor 5-level orthogonal test was designed to discuss the 4 factors 5-level orthogonal test:density, irrigation, fertilization and spraying the amount of fulvic acid. [Result]The results showed that the impact on irrigation water use efficiency of Populus euphratica was the main factors;the order of the intensity of the factors affecting water use efficiency of Populus euphratica was: irrigation frequency> fulvic acid spray volume> density> fertilizer; [Conclusion]the best combination of improving water use efficiency of the Populus euphratica was irrigation cycle 1 time per 16 days, the planting density was 25 plant per pot the concentration of fertilizer was 1 mg/mL, the concentration of fulvic acid spraying was 1.6 mg/mL.
    A Brief Summary on Storage and Preservation Temperature of Jiashi Muskmelon
    CHE Feng-bin;Aiermaike;LI Ping;ZHANG Ting;PAN Yan;HU Bai-wen;XIAO Lei
    2010, 47(1): 98-103. 
    Abstract ( )  
    [Objective and Method]The present experiment was carried out to screen the suitable temperature for Jiashi Muskmelon 1℃,2℃ and 3℃ to study the post-harvesting respiration tate ethylene production,soluble solids content,total sugar content,total acid content,hardness,pulp cell membrane permeability, etc.[Result]The result showed that in the storage process, soluble solids content and total sugar content of Jiashi Muskmelon had dropped slightly, total acid content had dropped obviously in the middle and late storage process; Under 1 ℃,it can maintain hardness of Jiashi Muskmelon,delay the time of respiration peak,reduce respiration rate,last out the lower electrical conductivity,inhibit cell senescence and reduce decay. [Conclusion]Under lower temperature, it is of great importance to devote every effort to maintain the surface of Jiashi Melon.
    Effect of Different Air Condition on the Quality and Physiological Activity of Postharvest 86-1 Melon During Storage
    LI Ping;CHE Feng-bin;HU Bai-wen;Aiermaike;ZHANG Ting;PAN Yan;XIAO Lei
    2010, 47(1): 104-109. 
    Abstract ( )  
    [Objective and Method]The three kinds of storage air condition:O_2+CO_2:3;+0;;O_2+CO_2:4;+1;;O_2+CO_2:5;+2; were designed to study the effect of the different air condition on the respiration rate,ethylene production,soluble solids content,total sugar content,total acid content,hardness,pulp cell membrane permeability, etc of the 86-1 melon during storage for choosing a better storage condition. [Result]The results showed that under 3 ℃,different air condition can not control the 86-1 melon's respiration rate and reduce the ethylene production .Different air condition can keep the 86-1 melon's better flavour and total sugar,acid content. [conclusion]More CO_2 content will be better for the 86-1 melon's storage, such as CO_2/O_2=2/5 was better than CO_2/O_2=0/3,whose reason should be further discussed in the future.
    Research on Botanical Characteristers and RAPD Marker of Thomson Seedless Bud Variation
    YAN Peng;LUO Qiang-wei;LIAO Kang;SUN Feng
    2010, 47(1): 110-114. 
    Abstract ( )  
    [Objective and Metnod]The study was carried out to observe the botanical and biological characters of thomson seedless bud variation and analyze young leaves by RAPD,using thomson seedlings as the control.[Result]There were different variations in bud variation of botanical characters.It was found that there was a bud variation with tetraploid in the test.[Conclusion]Through RAPD analysis, by using 470 random 10-alkali primers, RAPD polymorphism were detected from 30 samples. The polymorphism information could be provided with 3 random primers.
    Analysis on Spatial Distribution Pattern of Tamarix Ramosissima Population in Different Habitat
    CHEN Jin-xing;YIN Lin-ke
    2010, 47(1): 115-120. 
    Abstract ( )  
    [Objective]Tamarix ramosissima population mainly is divided into four kinds of habitat distributed types in the nature:the sandstorm locally born boundary, the meadow soil, spits adds according to the earth and the salt clay. [Method]The article used the pattern analytic method to analyze the different criterion spatial distribution pattern of Tamarix ramosissima in the different habitat. [Result]The result indicated that:①The sandstorm habitat type in criterion of 2-7 m was the colony distribution,when the criterion was transcended by 7 m,it was the random distribution.②Under all criterion (t),Tamarix ramosissima population of the meadow soil habitat type were all obviously deviated the colony distribution, and assumed it to be the random distribution;③The salt clay habitat type in criterion of 6~7 m was the colony distribution, but the criterion between 1~6 m, was obviously assumed it to be the random distribution; When the criterion transcended 7 m,it was the random distribution.[Conclusion]The research analysis result provided the basis for determining horizontal structure disposition pattern of Tamarix ramosissima population in different habitat in the Tamarix garden construction.
    Effect of Sulphur Fertilizer on Morphological Characteristics,Disease Resistance and Yield of the Garlic
    HU Fang;WANG Pu;SHI Shu-bing;NIE Wen-kui;GUO Fei;LIU Zheng-xing;YUAN Jin-ye
    2010, 47(1): 121-125. 
    Abstract ( )  
    [Objective and Method]The field experiment was conducted to study the effect of sulphur fertilizer using garlic plant height, stem diameter, leaf area per plant,rate of rust diseased plant, yield of the bulb and the flower stalk of garlic by use of the Xinjiang White Garlic as material.[Result]The result showed that sulphur fertilizer could accelerate the garlic's growth and development in Xinjiang Aheqi garlic area, increase garlic plant height, the stem diameter and the leaf area per plant, enhance the capacity of rust resistance,increase yield of the bulb and the flower stalk of garlic. It is better that the application of sulphur fertilizer was 12 kg/667m~2. To compare with no sulphur , the garlic plant height was increased by 7.8;, the stem diameter increased by 17.5;, the leaf area increased by 33.3;, the rate of rust diseased plant reduced by 80;, the bulb yield per unit area increased by 14.1;, the yield of the flower stalk of garlic increased by 16.1;. When the sulphur was within 0-12 kg/667 m~2,[Conclusion]the yield of the garlic was increased with the increasing of sulphur fertilizer.However, when the sulphur was exceeded by 12 kg/667 m~2, the yield was decreased.
    Study on Isolation and Identification of Endophytes in Yam Rhizome
    ZHANG Zhi-dong;XIE Yu-qing;CHU Min;GU Mei-ying;SONG Su-qin;TANG Qi-yong;MAO Jun
    2010, 47(1): 126-129. 
    Abstract ( )  
    [Objective and Method]Ten endophytes were isolated from yam rhizome, using five different isolation media. Four microbes were chosen for 16S rRNA gene sequence and systematic analysis.[Result]Results of 16S rRNA gene sequences blasting showed that the strains were within Erwinia genus and Bacillus genus, no actinomycete and fungus was found, which indicated that there was a poor diversity of endophytes in yam rhizome. The max homology of 16S rRNA of the produced polysaccharide strain SS2 product from yam rhizome leaching solution and model strain Erwinia pyrifoliae Ep1/96T was 97.1;.[Conclusion]which suggested the strain may be a novel species.
    Studies on SCP Preparation by Saccharomyces cerevisiae B_(188) Using Bean Dregs
    ZHANG Qiang;MA Qi;XUE Sheng-yun;CHEN Wei-feng;QIN Chen
    2010, 47(1): 130-134. 
    Abstract ( )  
    [Objective and Metnod]The objective of this paper was to investigate the technological conditions of SCP producton by Saccharomyces cerevisiae B_(188) to improve the feeding value of bean dregs and to relieve the shortage of forage by use of the bean dregs as raw medium.[Result]Optimization of the mediums and the cultivating conditions and the technology was determined.The ratio of bran and bean dregs was 1∶6,the initial pH 5.0,ammonium sulfate and added amount were 3.0; and 0.2; respectively,the inoculation volume were 12;,the cultivate temperature was 30℃and the period for 48 h.In the optimum conditions, the content of SCP synthetic was up to the maximum, protein content was 36.19;.[Conclusion]The technology provides a good basis for large scale production of bean dregs feed.
    A Study on Morphological Characters and Growth Expression of Eleven White Clovers
    QIE Cai;LI Wei-jun;ZHU Hao
    2010, 47(1): 135-140. 
    Abstract ( )  
    [Objective and Method]Eleven materials were primarily studied on their morphological characters,growth stages,production etc.[Result]The results showed that the variance of white clover was great.Among them,5,6,10,11 had high plant big leaves,high yield,fair growth expression.The material 8,9 had low plant,small leaves,short green period,low yield and relatively poor growth expression ,others were in the middle;Based on the cluster analysis of main morphological traits.,all the materials were divided into four types.[Conclusion]Three materials were screened, which could serve as green, ecological protection and forage species.
    Study on Absorption Law of Nitrogen,Phoshorus and Potassium of Cotton Plant under Different Nitrogen Application Rate
    ZHAO Shuang-yin;LIN Zhong-dong;LI Xiao-bin;XU Wen-xiu;LI Yin-ping;HOU Song-shan;YANG Tao
    2010, 47(1): 141-145. 
    Abstract ( )  
    [Objective and Method]The purpose of this research was to reveal the absorption law of nitrogen,phosphorus and potassium nutrient by cotton plant under different nitrogen fertilizer rate.[Result and Conclusion]The results demonstrated that when there was minimum accumulation of nitrogen,phosphorus and potssium of cotton plant with different treatments in the seedling stage of cotton,it was increased in bud stage,it reached a peak in flower bolling stage,but it was decreased in boll opening state.Among all the treatments,the productive period of accumulation of nitrogen,phosphorus and potassium treated with N_2 was the longest,which reached 54-100 d,57-120 d,44-89 d,respectively after sowing,which was earlier and longer than that of other treatments.The nitrogen fertilizer rate of the highest seed cotton yield was 348.32 kg/hm~2,the economic nitrogen fertilizer rate was 320.46 kg/hm~2.
    Analysis on Energy Dynamic State of Oasis Agriculture Eco-economic System in Aksu Region
    LI Yun-bin;XU Wen-xiu;WANG Jiao;ZHENG Ying-da
    2010, 47(1): 146-151. 
    Abstract ( )  
    [Objective]The aim of the study was to analyze the energy input,energy output,environmental load rate and index of sustainable development of the oasis agriculture eco-economic system in Aksu region.[Method]by the use of energy theory method from 2002 to 2007.[Result]The result showed that the total energy input,total energy output wre increased year by year.The total energy input in 2007 was increased by 50.7;,compared with that in 2002.The total energy output was increased by 160.13;,in which the output of farm porduction and forestry and fruit growing was faster increased.The energy output of farm production was kept in lower level about 0.05;The environmental load rate was incresaed year by year,which reached 8.32 in 2007,which was 2.97 times as average level of the whole China.The energy index of sustainable development was decreased.[Conclusion]Therefore,the new environmental resources should be proteced and developed to keep the agricultural eco-economic system in the sustainable developing state.
    Analysis of Landscape Pattern Change in Yuli Countyof the Lower Reaches of Tarim River
    HE Long-mei;CHENG Xia;SHI Qing-dong;CHANG Shun-li
    2010, 47(1): 152-156. 
    Abstract ( )  
    [Objective and Method]Through the use of modern 3S Technology , mathematical software and graphics software, based on vector map of multi-temporal remote sensing images, this paper tries to use GIS data input, output, management and spatial overlay analysis function to systematically analyze the changes in ecological pattern of Yuli County in middle and lower reaches of the Tarim River in 1990-2005.[Result]Since 1990, the basic distribution pattern of the landscape in Yuli County has not changed the desertification into a substrate, farmland and grassland into the main embedded block. [Conclusion]Landscape diversity index and evenness index were reduced, the degree of landscape heterogeneity was decreased , the dominance index was increased, the landscape is under the control of a few types of landscapes.
    Evaluation on the Land Ecological Security of Huocheng County in Xinjiang
    YUAN Lin;LIU Zhi-hui;LI Min
    2010, 47(1): 157-162. 
    Abstract ( )  
    [Objective and Method]The indicator system of land ecological security were established based on the P-S-R model, the weight of the indicator was determined by the method of mean square deviation, and the land ecological security were calculated from 1995 to 2005. [Result]The result showed that the change of the land ecological security in Huocheng County had experienced three stages: decrease,increase and re-decrease. [Conclusion]On the basis of the analysis of the causes, the strategies of the construction of land ecological security in Huocheng County were brought forward.
    Effects of Cotton Cropping Cotton Soil on Microorganism and Soil Enzyme Activity
    SHI Chong;LIANG Zhi;XU Wan-li;SHAN Hong-bin;JIA Hong-tao;WANG Li
    2010, 47(1): 163-167. 
    Abstract ( )  
    [Objective and Method]The studies were carried out on the effects of 5, 10, 15 and 20 years continuous cropping cotton soil on the microbial quantities, activities and enzyme activities in Xinjiang.[Result]The results showed that continuous cotton cropping has great effect on the number of its soil microbes and soil enzyme activities. With increasing year of cropping, the amount of bacteria, actinomycetes and fungi had a slight decrease. The amount of bacteria in the soil microorganisms proves to have the absolute superiority over actinomycosis and fungi. With increasing year of cropping, the B/F had a slight decrease. [Conclusion]The soil urease, phosphatase,invertase of cotton field with different cropping years were determined.The results demonstrated that the change of urease and invertase were similar to one another,which was increased with the increasing of continuous cropping years,but the activity of phosphatase was decreased first,then was increased with the increasing of continuous cropping years.
    Study on Spatial Variability of Soil Water and Salt under the Two Vegetation Coverages
    LI Bao-fu;XIONG Hei-gang;ZHANG Jian-bing;LONG Tao
    2010, 47(1): 168-173. 
    Abstract ( )  
    [Objective and Metnod]This paper comparatively analyzes the characteristics of the spatial variability of soil profile average water and salt content in the bare land and vegetational area using the typical salinizational area of oasis-desert ecotone in Qitai as the study area.[Result]The results showed that the soil water and salt content of bare land and vegetational area obey normal distribution; soil salinity belongs to moderate variability with a stronger spatial autocorrelation. Soil moisture variability in the bare land belongs to weak variability with moderate intensity of the spatial autocorrelation; vegetational area belongs to medium (weaker) variability with strong spatial autocorrelation. Bare land correlation distance is about 1/10 of vegetational area. With soil depth increasing, the activity changes of soil salinity in bare land and vegetational area are as follows: active layer, sub-active layer and stabler layer. Soil moisture change and its relationship with salt content are inconsistent, the surface of bare land affected largely by random factors is the most active and its correlation with salinity is also poorer,while the vegetation area in the middle and lower layer (60-80 cm) is the most active, which shows highly positive correlation with the salt. [Conclusion]Affected by random factors and structural factors (such as vegetation, micro-topography, etc.), the spatial variability of soil water-salt in the two vegetational coverages shows obvious difference and reflects significant active force and reactive force that the vegetation affects the water and salt distribution and the random factors of water and salt distribution.
    Effects of Phosphate on Phosphorus Conditions in Cotton-field Soil
    LIU Yong-gang;SHENG Jian-dong;CHEN Bo-lang;Kuerban·Maimaiti
    2010, 47(1): 174-179. 
    Abstract ( )  
    [Objective and Method]The aim of this text was mainly to study effects of DAP and TSP fertilizer different levels on the total P and available phosphorus in cotton-field soil at all growing stage.[Result]The results showed that phosphate fertilizer can increase soil available phosphorus, total phosphorus content different levels of phosphorus in the cotton seedling-stage and budded-stage the soil of application TSP were significantly more than DAP at soil total phosphate and available phosphorus; In the all growing stage of cotton , there were no significant difference between the cotton plant applied with DAP all levels and TSP(P_2O_5) at 75,150 and 300 kg/hm~2 levels and the cotton plant without aplication of phosphorus fertilizer.[Conclusion]So advice was given that application of phosphorus 150 kg/hm~2 could meet the needs of cotton plant.
    Effect of Using Top Nitrogen Fertilizer on Abscission Rate of Cotton Buds and Bolls
    GUO Yong;MA Xing-wang;YANG Tao;NIU Xin-xiang;WANG Bin;XU Yong-mei;LIU Hua
    2010, 47(1): 180-183. 
    Abstract ( )  
    [Objective and Method]The present study investigated the effects of nitrogen application rate on the abscission rate of cotton buds and bolls,on the basis of the survey of the abscission rate of cotton buds and bolls at different growth period.[Result]The results showed that if nitrogen application rate was too small or too large, the abscission rate of cotton buds and bolls would be different and only appropriate level of nitrogen application rate was used,there was no occurrence of serious abscission rate of cotton buds and bolls.[Conclusion]When irrigation volume was 5 250 m~3/hm~2, total N-amount of was 380 kg/hm~2, basal fertilizer was 40;,the first top dressing was 15;, the second top-dressing was 23;, the third top dressing was 22;, the abscission rate of cotton buds and bolls was the smallest in Kuerle of southern Xinjiang.The top fertilization of nitrogen of 22; at the late flower bolling stage and full bolling stage,it can make the abscission rate of cotton buds and bolls be the smallest and cotton yield be the highest.
    Research on Evaluation of Soil Fertilities of Farmland at County Level in Xinjiang based on GIS
    CHEN Shu-huang;GENG Qing-long;ZHANG Yun;ZHUANG Yu;DONG Ju-he;JIA Deng-quan;LAI Bo
    2010, 47(1): 184-188. 
    Abstract ( )  
    [Objective and Method]Based on the geographic information system(GIS) and the second general survey soil,overlaying the current landuse map with the basic farmland protection map and the soil map,forming 7 352 patches, which are used as the basic management and evaluation cells.This research selected twelve influencing factors from three aspects such as soil nutrient content,profile situation and land management,and the evaluation system and model of farmland fertility was built up. Thegrade of arable land productivity was formed according to the theory of Fuzzy Mathematics, Telphi Principle and Analytic Hierarchy Program,and the farmland fertility of Kuche county and analysis of its spatial distribution were evaluated.[Result and Conclusion]The results suggest that 73 553.52 hm~2 farmlands could be divided into six grades,the grade one was 7 713.07 hm~2,the grade two was 20 526.13 hm~2,the grade three was 14 621.53 hm~2,the grade four was 11 987.8 hm~2,the grade five was 11 302.33 hm~2 and the grade six was 7 402.53 hm~2,the fertility of grade one to six account for 10.49;,27.91;,19.88;,16.3;,15.37;,10.06; respectively.
    Research on Application Effect in Breeding of Molecular Marker in Sheep BMPR-IB Gene
    FANG Di;WU Jian-ping;XU Jian-feng;LUO Yan-ru;ZHANG Li-ping;WANG Lei;YANG Lian;LEI Zhao-min
    2010, 47(1): 189-194. 
    Abstract ( )  
    [Objective and Method]The polymorphism of bone morphogenetic protein receptor type IB gene (BMPR-IB gene) of 204 sheep of three breeds or strains ,which included Small Tail Han sheep,F_1 generation from German Merino(♂)×Small Tail Han sheep(♀) cross generation (DF1), German Merino ,was analyzed by PCR-sscp. The relationship between genotype and litter size was also identified.[Result and Conclusion]The results showed that the frequency of B allele in Small-Tail Han sheep, German Merino and DF1 was 0.660,0.532 and 0.600,there was no significant difference of distribution of gene frequency and genotype frequency in 3 groups.The litter size of BB,B+ and ++ was 2.86,2.27 and 2.08,BB were both significantly higher than B+ and ++ (P<0.05),and B+ were significantly higher than ++ type.
    Investigation on Infection of Sheep Eperythrozoon in Xinjiang
    ZHONG Fa-gang;HE Li-xiong;HUANG Xin;XU Xue-ping
    2010, 47(1): 195-199. 
    Abstract ( )  
    [Objective and Method]To learn the infection and epidemic status of eperythrozoon in Xinjiang,blood sheeting, Wright's stained blood smear with lightmicroscope and PCR were used to detect infection of the sheep eperythrozoon in Xinjiang, eight hundred and fifty blood samples were collected.[Result]The result indicated that there is serious infection of the eperythrozoon in Xinjiang; The infection rate reached 97; by use of blood sheeting and Wright's stained blood smear with light microscope.The infection rate reached 75; by PCR;Throughout the year there are occurrence of infections,no obvious seasonal one and no significant difference between the districts.Infection intensity was moderate or slight,but severe infection included two small peaks, respectively, from May to June and from November to December. [Conclusion]Conclusion was drawn that there was a high infection rate of Eperythrozoon in the population of Xinjiang sheep.The main predisposition of occurrence of this disease lies in the seasonal changes and forage conversion.
    Effect of Natural Grazing and Supplementary Feeding on Production Performance of Xinjiang Brown Cattle
    WANG Wen-qi;LIU Yan-feng;ZHAO Yun-jun;TANG Shu-zhen;ZHANG Yang;LIN Hong-kai;LI Hong-bo;OUYANG Wen
    2010, 47(1): 200-203. 
    Abstract ( )  
    [Objective and Method]Forty Xinjiang Brown Cattles were selected and divided into 4 groups:control group,test group 1,test group 2 and test group 3,which were fed with supplementary feeding by 0 kg/day/head,1.0 kg/day/head, 1.5 kg/day/head and 2.0 kg/day/head respectively.Effect of low-quality grazing pasture and supplements on Xinjiang Brown Cattle production performance was studied in the experiment.[Result]Results showed that the supplementary concentrated feed could increase milk production of Xinjiang Brown Cattle under the low quality grazing pasture conditions,it could inhibit the declining of milk production after high lactation period.[Conclusion]Supplementary feeding had no effect on lactoalbumin,batterfat rate and milk sugar when it was given.
    Design of the Land-use Classification System Based on Remote Sensing Image——A Case Study of Shihhotze Reclamation Area
    XU Xi-pan;CAO Wei-bin;LIU Jiao-di;LI Hua
    2010, 47(1): 204-210. 
    Abstract ( )  
    [Objective and Method]In order to protect, develop and utilize the land resources effectively, and grasp the true, accurate and real-time land-use information, the article takes Shihhotze reclamation area as an example to design an appropriate method of developing land-utilization -classification system which is based on remote sensing image technology and elaborate its significance.[Result]There were significant differences in the respect of the effect of the remote sensing image processing and analysis methods in the different goals and states.[Conclusion]In relation to the demonstration zones of significant regional features,the paper established the best way of land classification and model, designed the technological methods of developing land-utilization -classification system and also introduced the key research methods of data images processing,remote sensing images classified processing and classified precision assessment & methods validation.