Abstract: 【 Objective 】 To establish appropriate cryopreservation method to preserve shoot-tips of Populus euphratica dormant buds by using encapsulation-vitrification, thus preserving the germplasm resources of the endangered rare plant.【 Method 】Dormant shoot-tips were encapsulated in 3% calcium alginate beads. The main factors influencing the viability such as sucrose concentration, preculture time, different vitrification solution and the time in vitrification solution were studied.【 Result 】The results showed that the high viability could be obtained when alginate beads were precultured for 24 h in the 0.8 mol/L sucrose and the beads were dehydrated with vitrification solution 40% Gly + 45% (0.4 mol/L) sucrose + 10% PEG (4,000) + 5% DMSO for 30 min at 25℃. 【 Conclusion 】Under optimized conditions, the viability of dormant shoot-tips can reach 77% at the most. After cryopreservation, the dormant shoot-tips can grow directly and the genetic stability can be maintained. It is feasible to freeze the dormant shoot tips of Populus euphratica by liquid nitrogen.