Abstract: 【 Objective 】 To predict and analyze bioinformatics function for the GMD protein of Brucella, and to identify its immunogenicity and then the immune effect was evaluated on mouse model.【 Method 】The structure and function of ManB protein was analyzed by bioinformatics tools. The GMD gene of Brucella was amplified by PCR and the recombinant plasmid was transformed into E.coli BL21 (DE3) after being connected with pET-28a expression vector. The expression of target protein was detected by SDS-PAGE and its reaction was detected by Western Blot. Then the immunogenicity and immunoprotection were evaluated by mouse immunoassay and challenge experiments.【 Result 】Bioinformatics analysis showed that the protein had no transmembrane domain structure and no signal peptide. There were 15 antigenic determinants and its secondary structure was mainly α-helix. And the three-dimensional structure of the protein was constructed by Phyre2 Server. SDS-PAGE analysis showed that the fusion protein was about 43.7 ku. Western Blot showed that the protein could react with sheep serum against Brucella, indicating it possessed reactogenicity. Indirect ELISA results showed that the mice in the experimental group produced antibodies at 7th, 14th and 21st after immunization and the antibody level increased with time. The results of immune protection test showed that the spleen index and CFU of the spleen were lower than those in the PBS group. 【 Conclusion 】The GMD recombinant protein has good immunogenicity and can induce high level of specific antibodies after immunization in mice. After immunization, it can play a certain role in immune protection.