Abstract: 【 Objective 】 To investigate the resistance of Escherichia coli from different animals to commonly used antimicrobial agents in Yanqi County, Xinjiang. 【 Method 】Feces of piglets (aged 40 days and 60 days) were collected from the main farms in the county, and the feces of pregnant sows, cattle and sheep were collected, respectively. The minimum inhibitory concentration of Escherichia coli was determined by agar dilution method.【 Result 】The results showed that the resistance severity of fecal Escherichia coli from 40-day-old > fecal Escherichia coli from 60-day-old piglets > fecal Escherichia coli from pregnant sows. Escherichia coli from sheep and cattle had different degrees of resistance to detected antimicrobials. Escherichia coli from piglets was mainly resistant to 6-8 (40 days old: 89.2%, 74/83; 60 days old: 49.0%, 49/100), and pregnant sows was mainly resistant to 2-4 (63.3%, 62/98). Escherichia coli from cattle was mainly resistant to 1-2 (58.0%, 47/81) and sheep was mainly resistant to 0-2 (80.2%, 138/172).【 Conclusion 】Escherichia coli from different animals in the main farms had different degrees of resistance to the commonly used antibiotics in clinics in Yanqi County, Xinjiang, so in the breeding process, the rational use of antimicrobial agents should be taken into consideration in the case of bacterial resistance.