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    25 January 2017, Volume 54 Issue 1 Previous Issue    Next Issue

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    Genetic Effect Analysis of Main Breeding Targets of Three Lines Hybrids Cotton in Northern Xinjiang
    DAI Yong-qiang;ZHANG Xin-yu;SUN Jie
    2017, 54(1): 1-9. 
    Abstract ( )   PDF (1160KB) ( )  
    [Objective] This study aims to understand the genetic rules of main selection index of three lines hybrids of Xinjiang cotton with genetic effect analysis to screen high heterosis hybrid combination.[Method] Forty-two crosses were made between 7 sterile lines and 6 restorer lines by using 7 × 6NC Ⅱ cross design.The yield,lint percentage and fiber quality of F1 generation were used to carry out statistical analysis of genetic variance and combining ability.[Result] Yield was affected by both dominant and additive effects,and may be affected by overdominance effects.Lint percentage,length,micronaire and strength were predominantly affected by additive effect and party by partial dominance effect.In the sterile lines,3-248A showed the highest general combining ability,2-20A and 6-89A showed higher general combining ability,but other restorer lines showed low or negative general combing ability.In the restorer lines,12-278R showed the highest general combining ability,8-420R and 9-186R showed higher general combining ability,but other restorer lines showed low or negative general combing ability.In cross combinations,2 × 9 showed the highest specific combining ability,6 × 12,4 × 9,3 × 8 and 6 × 8 showed higher specific combining ability,exhibiting 23.33;,16.67;,15;,10.83; and 5; higher yield than that of the control,respectively,but other combinations showed low or negative specific combining ability.[Conclusion] Filter out the high advantages of hybrid 2 × 9,exhibiting 23.33; higher yield than that of control respectively,lint percentage and fiber quality of F1 generation made between 7 sterile lines and 6 restorer lines with 7 ×6NC Ⅱ cross design.
    On Characteristics of Boll Development of Xinluzao Series of Cotton Varieties and Their Relationship with the Yield and the Fiber Quality in Northern Xinjiang
    CHEN Zong-kui;TIAN Long-hai;SUO Xiao-xia;TIAN Jing-shan;LUO Hong-hai
    2017, 54(1): 10-19. 
    Abstract ( )   PDF (1465KB) ( )  
    [Objective] This experiment aims to explore the cotton boll development and the effect on fiber quality and yield of Xinluzao cotton varieties,which might be of great significance to construct high-yielding cultivation technique system and new variety cotton breeding in Xinjiang.[Method] Field experiments were carried out to study the characteristics of cotton boll development among 12 main cotton varieties used widely from 1980s to 2010s,and the boll length,diameter,volume,single boll weight,yield and fiber quality during development were determined.[Result] During the evolutional succession of Xinluzao cotton varieties,boll length,boll diameter,boll volume and boll weight decreased by 2.79;-16.92;,2.27;-13.28;,10.15;-35.9; and 11.5;-18.9;,respectively;Bolls number per plant and seed cotton yield increased by 4.0;-45.29; and 8.75;-28.04;,respectively.From 1980s to 2000s,fiber length and fiber strength increased;during the period from 2000 to 2010,there were no obvious changes.The correlation analysis showed that boll length,boll diameter and boll volume were significantly negatively correlated with the bolls number per plant,seed cotton yield,fiber uniformity and micronaire,and significantly positively correlated with single boll weight,fiber length and specific strength.The further path coefficient analysis showed that boll length and boll diameter went against increasing bolls number per plant,but both helped to improve fiber strength and fiber length.[Conclusion] The increase of yield was mainly attributed to the increase of boll number per plant during the evolutional succession of Xinluzao cotton cultivars,and the length and diameter of 0 ~21 d were closely related to the yield and quality.
    Correlation Analysis between Hyperspectral Vegetation Indices and Photosynthetic Parameters of Cotton under Different Water Treatments
    GUO Xiao-fei;HUANG Chun-yan;TIAN Chun-yan;LIU Xin-yue;WANG Deng-wei
    2017, 54(1): 20-26. 
    Abstract ( )   PDF (1168KB) ( )  
    [Objective] To establish a correlation model between cotton hyperspectral data and photosynthetic characteristic parameters in the hope of providing a scientific foundation for effective,rapid,non-destructive diagnose and surveillance of cotton growth process in large area.[Method] Using ASD hyperspectral radiometer and Li-6400 photosynthetic instrument,the hyperspectral data of the two varieties Xinluzao No.13 and No.33 in key growth stages and photosynthetic parameters were obtained by five water treatments:net photosynthetic rate (Pn) and stomatal conductance (Gs) were calculated using hyperspectral data to obtain the normalized difference vegetation index (NDVI),ratio vegetation index (RVI) and the second modified soil adjusted vegetation index (MSAVI2) of the two cotton cultivars,thus establishing the linear,logarithmic and power function correlation equations of Pn and Gs,respectively.[Result] The results showed that three type function models were of significant and extremely significant correlation,and the R value of the equation of the three models in the two varieties RV[and Pn and Gs was higher,among which,using Xinluzao 33 RVI and Pn,Gs power function equation to estimate the Pn and Gs and carry on correlation analysis of the predicted value of Pn and Gs with the measured value,it was found that R value reached a very significant level (R measured pn =0.827,RMSE =1.089,R measured gs-estimation gs =0.586,RMSE =0.138,n =20,P < 0.01).The estimation accuracy of the model equation was greater than 80;.[Conclusion] There was a significant correlation between the spectral vegetation indexes and photosynthetic parameters of the two cotton varieties under different water treatments and the correlation model could be used to estimate the Pn and Gs,and real-time monitoring of the growth status of cotton.
    Research of Drip Spring Wheat Nitrogen Fertilizer Model Based on Nitrate
    LIU Xiao-fang;ZHANG Ze;L(U) Xin;NIE Ying-bin
    2017, 54(1): 27-32. 
    Abstract ( )   PDF (1149KB) ( )  
    [Objective] To adopt crop nutrition diagnosis technology and establish fertilizer nitrogen diagnosis period model of drip irrigation wheat based on the plant nitrate.[Method] Determine plant nitrate content of key growth period of drip irrigation wheat through the relationship between nitrate concentration and N application rate and the yield and establish the fertilizer nitrogen diagnosis model of the key remaining period.[Result] There was a very significant correlation between nitrate content of drip irrigation wheat plant stem base and the yield at different development stages,and the nitrate critical value of jointing stage and booting stage were 2 782.5 and 2 553.06 mg/kg,which established spring wheat jointing stage and booting stage fertilizer fertilization model of drip irrigation wheat.[Conclusion] The plant nitrate of irrigation wheat the of jointing stage and booting stage as a diagnostic index was used to establish the corresponding application model and calculate the different nitrate test values corresponding to the amount of nitrogen fertilizer at the drip spring wheat jointing stage and booting stage.
    Effects of Artificial Aging on Seed Vigor, Starch Decomposition and Endogenous Hormones of Barley in the Early Germination
    WANG Feng;QI Jun-cang;ZHENG Xu-guang;GUO Ya-nan;GONG Lei;WANG Shao-yu;CHEN A-long;HUANG Xiang-yi;LI Zhong-hao;SONG Rui-jiao
    2017, 54(1): 33-42. 
    Abstract ( )   PDF (1369KB) ( )  
    [Objective] [Method] This study aims to explore the effects of artificial aging on seed vigor,starch granule,starch mass fraction and analyze the activity and endogenous hormones in the endosperm of barley seeds with high temperature and high humidity artificial aging treatment in the early stage of germination.[Result]The results showed that:The germination indexes and embryo vitality of 4 varieties decreased gradually with increasing aging time.Artificial aging increased the small starch granules and reduced large starch granules of endosperm in the germination 72 h.In addition to that,artificial aging increased starch mass fraction during the germination,reduced the activity of α-amylase and β-amylase of all barley and there were significant differences between the aging 15 d and the control.Content of GA3 was lower than that of CK in the germination 24 h but higher than that of the CK in the germination 48 to 72 h.However,contents of ABA were always higher than those of the CK in the all germination process.[Conclusion] The balance between GA3 and ABA was broken by aging,which resulted in the decrease of amylase activity and the slow-down of starch decomposition,so then barley seed vigor was decreased and the germination inhibited.
    Relationship between Epicuticular Wax Components and Its Content and Drought Resistance in Barley Leaf
    ZHAO Dong-bin;ZHANG Hai-lu;WANG Xian;QI Jun-cang;HUI Hong-shan;LIN Li-hao;WANG Feng;ZHENG Xu-guang
    2017, 54(1): 43-50. 
    Abstract ( )   PDF (1063KB) ( )  
    [Objective] In order to understand the influence of water stress on plant layer of epicuticular wax and its component contents and the relationship between the leaf layer of epicuticular wax and plant drought resistance.[Method] The paper studied the variation of flag leaf and layer of flag leaf epicuticular wax content in two barley with strong drought resistance and 2 varieties with weak drought resistance.[Result] The results showed that the epicuticular wax content of barley leaves under drought stress increased and that of drought tolerant cultivars increased more than that of drought sensitive cultivars,and after drought stress,the main physiological parameters of photosynthesis were decreased.The main components of epicuticular wax of barley leaves were alcohols,alkanes,acids,aldehydes,esters and ketones;the increase of epicuticular wax of barley leaves under drought stress was mainly due to the increase of alcohols,esters and ketones while the increase of alkanes was not obvious;net photosynthetic rate of barley leaves was positively correlated with the alcohols content and negatively correlated with the alkanes content,indicating that alcohols and alkanes as the main components of epicuticular wax of barley leaves had significant auxo-action to photosynthesis;stomatal conductance was negatively correlated with total wax content and alcohols content.[Conclusion] The content of alcohols,esters and ketones of epicuticular wax in barley leaf will be increased under drought stress condition,then the photosynthetic characteristics is regulated by wax components to maintain a high photosynthetic rate,which can improve the drought tolerance of plants.
    Effects of Different Chemical Flower Thinning Agents on the Quality of Prunus salicina × armeniaca
    WEI Ya-jun;XU Ye-yong;FENG Bei-bei;LI Wen-wen;WANG Ming;YANG Hong-li;NIU Ying-ying;LIAO Kang
    2017, 54(1): 51-59. 
    Abstract ( )   PDF (1073KB) ( )  
    [Objective] In order to research the influence of chemical methods on the flower thinning effect and quality of Prunus salicina × armeniaca to screen the suitable chemical agent and concentration and to propose a simple method of flower thinning and control reasonable crop load.[Method] Three cultivars of Prunus salicina × armeniaca were taken as materials,different concentrations of three kinds of chemical flower thinning agents were sprayed at full-bloom stage and then the fruit setting rate and quality were determined.[Result]The results showed that when Fengweihuanghou was sprayed one time 20 mg/L NAA and 0.3°Be lime-sulfur at full-bloom stage,Konglongdan was sprayed 30 mg/L NAA and 0.4° Be lime-sulfur and Weidi was sprayed 30 mg/L NAA and 0.4°Be lime-sulfur,the fruit setting rate of these treatments was lower than that of the CK,and the fruit shape index,soluble solid content and sugar content and acid content were significantly improved.[Conclusion] Spraying one time 20 ~30 mg/L NAA and 0.3 ~0.4° Be lime-sulfur has better effects of flower thinning at one day or two days of full-bloom stage and can improve the quality of fruits.
    Correlation Analysis of Solid Stone Cell Content and Expression of POD4 Gene in Korla Fragrant Pear
    LI Wen-hui;FENG Jian-rong;TANG Zhang-hu;DONG Sheng-li;XU Lin;ZHANG Shi-kui;ZHANG Yan
    2017, 54(1): 60-65. 
    Abstract ( )   PDF (1154KB) ( )  
    [Objective] To study the correlation of stone cell content and POD4 gene expression in rough skin pear and normal skin fruit of mature Korla Fragrant Pear in the hope of providing molecular basis for improving the fruit quality.[Method] Rough skin fruit and normal skin fruit of Korla Fragrant Pear during the mature period were used as materials,and the firmness and the content of stone cell were determinated.Total RNA was extracted from fragrant pear fruit pulp,quantification expression of POD4 gene was determinated by Real-time fluorescent quantitative polymerase chain reaction (qRT PCR),the correlation of the firmness and the content of stone cell and quantification expression of POD4 gene were analyzed.[Result] The content of fruit stone cell was between 0.4 and 0.52 gram,pear firmness was between 245.22 and 314.71 PA and quantification expression of POD4 gene in mature pear was between 106.89 and 743.29.[Conclusion] The content of stone cell in rough skin fruit is 1.3 times than the normal skin fruit,the hardness was 1.28 times,POD4 gene expression was 6.59 times.The correlation between the content of stone cell and the quantification expression of POD4 gene was significantly positive (r =0.823),and correlation between the content of stone cell and the fruit hardness was positive (r =0.783).The correlation between quantification expression of POD4 and fruit firmness was positive (r =0.770).Therefore,the level of expression of POD gene can be used as a reference index for the identification of the content of stone cell.
    Interaction Effects of Water and Fertilizer on the Yield and Physiological Condition of Zizyphus Jujube
    FU Yan-bo;WANG Cheng-fu;HUANG Jian;TANG Ya-li;WANG Zhi-guo;FENG Yao-zu
    2017, 54(1): 66-75. 
    Abstract ( )   PDF (1437KB) ( )  
    [Objective] To understand the effects of water stress and nutrient stress on the yield of Zizyphus Jujube in Xinjiang.[Method] Based on an interactive test of two water gradients and two nutrient gradients,soil moisture content,red date yield as well as nutrients and chlorophyll fluorescence of red date leaves from red date sample plots in Akesu City (Xinjiang) under different water and fertilizer conditions were tested.[Result] (1) Soil moisture content increased with the increase of irrigation amount;(2) With the increase of water and nutrients,Zizyphus Jujube yield,N,P and K contents in leaves,maximum photochemical quantum yield of PSⅡ,PSⅡ actual photochemical efficiency (φPSⅡ),photochemical fluorescence quenching coefficient (qP) and photosynthetic electron transport rate increased,while the initial fluorescence (Fo) and non-photochemical quenching coefficient NPQ decreased;(3) High water and nutrient contents contributed to higher Zizyphus Jujube yield and better physiological indexes than other conditions.[Conclusion] The addition of water alone is more beneficial to the growth of red date than the single nutrient,and water is the main factor to affect the yield of jujube.
    Effect of Different Fruit Set Heights, Shoot Densities and Canopy Thicknesses on Microenvironment and Berry Quality of “厂” Shape Cabernet Sauvignon
    ZHANG Wen;ZHONG Hai-xia;ZHANG Fu-chun;HAN Shou-an;XIE Hui;WU Xin-yu;Aiermaike Caikasimu;PAN Ming-qi
    2017, 54(1): 76-87. 
    Abstract ( )   PDF (1262KB) ( )  
    [Objective] In this article,the effects of fruit set height,shoot density and canopy thickness on microenvironment around berry and fruit quality of Cabernet Sauvignon with ‘厂’hape training structure were studied by L9 (3') orthogonal test design in the hope of providing a theoretical basis for choosing canopy man-agement standards for wine grape in the northern piedmont producing area of Tianshan mountains.[Method] 5a old Cabernet Sauvignon was taken as the testing materials,L9 (34) orthogonal test design was used,3 factors such as fruit set height(A),shoot density (B)and canopy thickness (C) were set and each factor had 3 levels (A:30 cm,50 cm,80 cm;B:25,20,15 bud/m;C:100 cm,80 cm,60 cm),altogether 9 treatments.The temperature,humidity and spectral composition around berry were investigated during the berry veraison,the weight of berry,number of seed,pericarp weight/berry weight,seed weight / berry weight,soluble solid,titratable acid and tannin were measured during the mature period.[Result] Fruit set height,shoot density and canopy thickness influenced the fruit quality through the changing temperature,humidity and spectral composition around berry.Among them,the fruit set height was the main influence factor on spectral composition around berry,soluble solid and titratable acid.The content of soluble solids and titration acid were the highest when the height was set at 50 cm.The content of tannin and fruit was mainly affected by the shoot density,so 25 bud/m shoot density produced the highest value.Canopy thickness was the main influence factor on the number of seed and seed weight/berry weight,so when the thicknes was 80 cm,more number of grains would be fouind.[Conclusion] The order of the influence on berry quality from high to low is fruit set height,canopy thickness and shoot density,among which,the fruit set height 50 cm,canopy thickness 80 cm and shoot density 25 shoot/m have produced the best berry quality.
    Cloning and Sequence Analysis of Anthocyanin Biosynthesis Related Genes (PgANS, PgCHS and PgACT) in Pomegranate (Punica granatum L.)
    WU Zhong-hong;DU Juan;Atawulla Tiemuer;ZHOU Jiang;XU Jing;WU Bin
    2017, 54(1): 88-94. 
    Abstract ( )   PDF (1752KB) ( )  
    [Objective] To obtain PgANS,PgCHS and PgACT genes in Kashgar pomegranate kernels and analyze their sequence.[Method] Xinjiang pomegranate in Kashgar was selected as the materials.The total RNA of pomegranate Kernels was extracted with Phenol-chloroform.The first cDNA was synthesized with TIANScript kit,and amplified to cDNA by RT-PCR using special primer.The pMD19-T vector linked with the cDNA fragment was transformed into Escherichia coli DH5α,and the positive clone was sequenced.[Result] The fragment of PgANS was 474bp,GenBank accession number was KT779433.Compared with Punica granatum L.which the accession numbers were KF841619,GU376749 on NCBI,the PgANS homology of nucleotide sequence was 99;.At the same time,the sequence analysis demonstrated that the deduced nucleotide sequence shared 85;,79; and 76; homology with that of Ipomoea batatas (GU598212),Morus alba (JF499385) and Fragariax ananassa (AY695817);the fragment of PgCHS was 140bp and the PgACT was 148bp.Compared with Punica granatum L.(JF747148,GU376750) on NCBI,the PgCHS homology of nucleotide sequence was 99;,and the PgACT was 93;.[Conclusion] The PgANS,PgCHS and PgACT genes have been obtained,which has laid the foundation for further studying the influence of storage conditions on the anthocyanins of Punica granatum L.kernels.
    Effects of Conservation Tillage Measures on Soil Moisture in Jujube Orchard and Yield of Jujube
    HU Qiang;WAN Su-mei
    2017, 54(1): 95-103. 
    Abstract ( )   PDF (1238KB) ( )  
    [Objective] In order to explore the conservation tillage measures of jujube production in arid regions and promote the red jujube industry efficient,green,sustainable development in Southern Xinjiang.[Method]The soil water content from 0-100 cm soil layers in jujube orchard was evaluated and the yield of jujube and water use efficiency were analyzed by setting different conservation tillage methods.[Result] (1) The average soil water content with the treatment of tillage straw mulching (TS) reached 16.55 ;,which was 4.93;,1.97; and 7.34; higher than those of rotary tillage straw mulching (RS),no-till straw mulching (NS) and no-till bare land (NB) respectively.(2) In the whole growth period,soil water content of 0 to 100 soil profiles showed an overall increasing trend after the initial decrease.Tillage straw mulching (TS) obviously improved the soil water content of 60-100 cm soil profiles.(3) The yield and water use efficiency of the straw mulching (TS) were the highest,which were 10,500 kg/hm2 and 21.67 kg/hm2,respectively,compared with RS,NS,NB processing production,they were increased by 1,650 kg/hm2,3,000 kg/hm2,3,150 kg/hm2,and water use efficiency was increased by 4.33 kg/(mm · hm2),7.94 kg/(mm · hm2) and 8.07 kg/(mm · hm2).[Conclusion] A preliminary exploration of protection tillage straw mulching cultivation measures is more conducive to the implementation of jujube in extreme arid area.
    Strawberry Growth under Different Nitrogen Levels
    ZHENG Hong-bo;LI Ya-li;GENG Qing-long;ZHENG Zhong
    2017, 54(1): 104-109. 
    Abstract ( )   PDF (1140KB) ( )  
    [Objective] To study the best quality and yield of strawberry under different nitrogen levels in greenhouses and determine the optimum N application concentration.[Method] The research object of this study was "Fenxiang".The growth,the fruits and the quality of strawberry under six different nitrogen application rates were studied under the same cultivation and management techniques.[Result] In the treatment of six nitrogen levels,the plant height,stem diameter,leaf area and yield of strawberry increased with the increase of the amount of nitrogen applied in the range of 2 ~ 7.5 mmol/L.But when the nitrogen application rate was over 7.5 mmol/L,the nitrogen utilization rate would decrease and the plant height,stem diameter,leaf area and yield of strawberry showed a trend of decrease.[Conclusion] Nitrogen application level at 7.5 mmol/L was the best concentration for strawberry in greenhouse soilless cultivation.
    Pollen Morphology, Viability and Stigma Receptivity of Iris scariosa Willd
    MA Yu-mei;ZHANG Yun;QIN Jing-yi;WANG Xiu-mei;ZHU Tian-tian
    2017, 54(1): 110-116. 
    Abstract ( )   PDF (1168KB) ( )  
    [Objective] To explore the dynamic variation rule with pollen viability and stigma receptivity of Iris scariosa Willd.,in order to provide a reliable basic data for sexual reproduction.[Method] Scanning electron microscope was used to observe pollen morphology,in vitro pollen germination and staining method were applied to test the pollen viability,and the stigma receptivity of different flowering period was evaluated with the benzidine-α-naphtho method,and the artificial-pollinate method was employed to test the best period of pollination.[Result] The pollen morphology of aperture is monocolpate,belonging to the distal single groove-quasi groove type,the outer wall of pollen surface had scattered columnar grains;The higher pollination held in the first flowering day and flowering after 4 h reached the highest peak,the stigma had receptivity from the first day to flowering after 32 h.[Conclusion] The pollen morpho-gy is a more primitive type,female flower matured before male,Pollen viability and stigma overlap.The day of flowering and the next day are the best time for artificial pollination.
    Effect of Neighboring Crops Distribution Patterns and Film Mulching on the Population Dynamics of Colorado Potato Beetle, Leptinotarsa decemlineata in Xinjiang
    LI Chao;DING Xin-hua;WANG Xiao-wu;GUO Wen-chao;Tuerxun Ahemaiti;HE Jiang
    2017, 54(1): 117-123. 
    Abstract ( )   PDF (1368KB) ( )  
    [Objective] The study focuses on clarifying the role of neighboring crops distribution patterns and plastic film mulching cultivation in ecological regulation of Colorado potato beetle.[Method] The present study compared the differences of Colorado potato beetle population in potato fields accompanied by different adjacent crops in existing cropping patterns.[Result] There were basically the same population dynamic patterns among different potato fields with other different kinds of adjacent crops,but there were significant differences among their population amount of Colorado potato beetle.Near the previous year's potato fields,the fields were damaged seriously,but the damage levels would be reduced with increasing distance between the potato fields.[Conclusion] Planting different kinds of crops influences the population dynamic of Colorado potato beetle to a certain extent and the ecological control of the Colorado potato beetle population can be carried out by adjusting the planting pattern.
    Real-time PCR for Detection of Lactobacillus rhamnosus Based on Dual Priming Oligonucleotide System
    WEI Shuang;MENG Ru;QIAN Yi-ke;SUN Kai;LIU Zhong-yong;YIN Jie-ping;ZHOU Guang-biao;WU Xi-yang
    2017, 54(1): 124-131. 
    Abstract ( )   PDF (1156KB) ( )  
    [Objective] To develop a method for the detection of Lactobacillus rhamnosus.[Method] The species-specific DPO (dual priming oligonucleotide) primers were designed based on thegyrB gene sequences of L.rhamnosus.A SYBR Green I real-time PCR assay based on DPO primers was developed for the detection of L.rhamnosus.The specificity and sensitivity of the assay have been estimated.[Result] The results showed that this method was of high specificity,and only two L.rhamnosus strains can be amplified.The other 10 L.spp.strains gave negative results.The detection sensitivity of this real-time PCR was more than 10 times higher than the conventional PCR.[Conclusion] SYBR Green I real-time PCR method established in this study can detect L.rharanosus accurately and rapidly in the microbial fertilizers and other probiotic products.
    The Screening and Identification of Antagonistic Actinomycetes Strains from the High Radiation Soil
    LI Yang;LI Jian-qiang;SONG Su-qin;WANG Jing;CHU Min
    2017, 54(1): 132-139. 
    Abstract ( )   PDF (1283KB) ( )  
    [Objective] This project aims to isolate and screen 70 actinomycetes strains from the high radiation soil of Xinjiang by antagonistic effect of 8 kinds of pathogenic bacteria such as the Valsamali Miyabe et Yamada.[Method] By the plate confrontation culture method,and through initial screening and repeated screening of antagonistic effect of 8 kinds of pathogenic bacteria,and according to the morphological characteristics of the strains of CH10,the physiological and biochemical characteristics and 16S rDNA sequence went through identification and analysis.[Result] The results showed that 8 kinds of important pathogens of Xinjiang crops,such as the Valsa mali Miyabe et Yamada,were screened out from 70 kinds of actinomycetes,which had good antagonistic effect called CH10.[Conclusion] Strain CH10 belongs to the genus streptomyces and it has good antagonistic effect on the Valsa mali Miyabe et Yamada,which has provided a foundation for the biological control of the disease.
    Hyperspectral Characteristic Analysis and Estimation of Total Nitrogen Content in Grey Desert Soil
    LIU Fan;MA Ling;YANG Guang;CHEN Jian-hua;MA Xue-lian;WANG Hai-jiang
    2017, 54(1): 140-147. 
    Abstract ( )   PDF (1236KB) ( )  
    [Objective] To solve the traditional soil total nitrogen determination methods such as complexity,time-consuming,energy consumption and other issues.[Method] Gray desert soil in Xinjiang was taken as our research object to study the spectral reflection characteristics of different soil total nitrogen and by using classical statistical and spectroscopy method,the remote sensing inversion models of soil total nitrogen were established and validated in the study area.[Result] The results indicated that the content of total nitrogen in soil was similar to those of different spectral reflectance.The spectral reflectance of the soil appeared near 780,1,800 and 2,140 nm wavelength.There was obvious absorption valley in the vicinity of 1,910 nm and the correlation between the total nitrogen content in soil and the original spectral reflectance was poor.The determination coefficients of first-order differential processing to original spectral reflectance were higher than original spectral reflectance and second order differential,the maximum R2 was 0.819,reaching a very significant correlation;By using the first order differential transform to extract the characteristic bands 667 and 1,414 nm,the estimation model of soil total nitrogen content was established,they were:Y =2,698.048 X667-1,062.149 X1414-0.015.The R2 was 0.75;The estimation model validation found that the R2 was 0.80.When the total nitrogen content was too large or too small,the model estimation error was relatively large,and the overall prediction accuracy was quite high.The results has provided a theoretical basis to improve hyperspectral remote sensing monitoring accuracy of soil nitrogen.[Conclusion] High spectral analysis technique has certain significance for prediction of soil total nitrogen content and rapid identification of soil total nitrogen content by using the estimation model can be achieved.
    Dynamic Analysis of Community Characteristics and Diversity of Grassland in the Northern Slope of Tianshan Mountain
    ZHANG Li;ZHANG Xian-hua
    2017, 54(1): 148-155. 
    Abstract ( )   PDF (1057KB) ( )  
    [Objective] The project aims to determine the height,coverage,density and aboveground biomass of the dominant plant species of grassland communities in different months and calculate the important values and analyze the dynamic change of diversity indexes in the northern slope of Tianshan mountain.[Method] The meter ampere quadrates of different grassland types were set based on the crossing method,and community characteristic parameters were monitored from spring to autumn.[Result] There were some differences in the important values of different grassland types.The whole trend of important value from may to september showed decreasing trend;especially forage grass of Stipa capillata,Festuca ovina decreased obviously than the forbs important value.The plant community height,coverage and aboveground biomass showed high trend in late spring and early summer while low from August to September.The statistical results showed that the trend of diversity indexes was increasing first and then decreasing and then increasing,such as Simpson index D and Shannon-Wiener index H,evenness index Esw and richness index R,And Simpson index D and Shannon-Wiener index H and species richness R to aboveground biomass were significantly positively correlated,and evenness index to aboveground biomass was not significantly negatively correlated.[Conclusion] The preliminary study of the quantity characteristics of the plant communities in different grassland types showed a trend of increasing first and then decreasing with the seasonal variation and the species diversity increased firstly and then decreased.The results can provide data for supporting the rational utilization of grassland.
    The Vertical Distribution Characteristics of Ili Zhaosu Grassland Chernozem Soil Organic Carbon at Different Altitudes
    Abudousaimaiti Naihemaiti;Aikebaier Yilahong;Saiyaranmu · Halipu
    2017, 54(1): 156-164. 
    Abstract ( )   PDF (1210KB) ( )  
    [Objective] This project aims to analyze the vertical distribution characteristics of Zhaosu grassland chernozem soil organic carbon content,carbon storage and density.[Method] Using Potassium dichromate external heating method to measure the soil organic carbon content in 0-100 cm soil depth at different altitudes and discuss their changes.[Result] Results showed that Ili Zhaosu grassland chernozem organic carbon content,carbon storage,carbon density revealed a trend of decrease with the increase of altitudes and the soil depths.Organic carbon content,carbon storage and density of 100 cm in the study area at the attitudes between 1,000-3,000 m were 64.70-616.78 g/kg,4.49 × 10-8-1.94 × 10-6 Pg/hm2 and 3.26-194.27 kg/m2,respectively.The typical grassland chernozem soil organic carbon content,carbon storage density and altitude were negatively correlated.(r =-0.92,-0.943,-0.95) and had significantly negative correlation with the soil layer thickness (r =-0.989* *,-0.968* *,-0.966 **).[Conclusion] Ili Zhaosu grassland chernozem soil organic carbon content,carbon storage and carbon density decreased with the increase of altitudes and depths in the profiles.
    Soil Bulk Density Variation of Grassland in Ili Valley and the Measurement Standardization
    ZHANG Wen-tai;CHEN Shi;LIU Yun-hua;SHENG Jian-dong;SU Yue
    2017, 54(1): 165-170. 
    Abstract ( )   PDF (1182KB) ( )  
    [Objective] The purpose of this project is to study the variation of soil bulk density in the horizontal and vertical directions and the influence of the gravel content on the determination results of bulk density in the hope of promoting the standardization of soil analysis and soil resource management.[Method] Based on field soil survey,56 soil profiles were dug in grassland of the Ili Valley.Soils were sampled by using core method at uniformly set soil layers,and each layer was repeatedly sampled five times.Gravel content was measured in lab when it existed at a certain soil layer.The coefficient of variation (CV) was used to quantitatively express lateral variation of soil bulk density.[Result] The mean CV values of soil bulk densities measured from five samples for all soil layers were lower than 8;.Gravel could improve soil bulk density by about 16;.Soil bulk densities of varied soil layers at vertical direction were significantly different,and bulk density increased along with increasing soil depth.[Conclusion] Soil layer should be rationally classified when studying bulk density of soil profile in grassland.In view of the fact that highly stony soils existed in grassland of Xinjiang,we suggest that the relevant standard for measuring bulk density should be modified.
    Simulation and Analysis of Land Surface Process on the Typical Oasis Cropland in Xinjiang
    JING Chang-qing;AN Sha-zhou
    2017, 54(1): 171-178. 
    Abstract ( )   PDF (1443KB) ( )  
    [Objective] The project aims to verify the model performance of CoLM for the simulation on the typical oasis cropland ecosystem in Xinjiang,and analyze the characteristic of the land surface process.[Method] Two years of meteorological data from 2009 to 2010 in CN-Wul station were used to drive the land surface model,verify the model accuracy in this cropland site,and analyze the energy distribution characteristics,the daily and seasonal variations of this cropland ecosystem.[Result] The results showed that the energy flux:Rnet > Qle > Qh,the latent heat flux was the main part of the energy distribution;CoLM showed a good performance to the typical cropland ecosystem.[Conclusion] This land surface model can correctly describe the diurnal variation and seasonal variation of the energy flux,but of sensible heat flux in the field growth season is not ideal,because the simulation value is higher than that of the observations.
    Determination of Freezing Point of Raw Donkey Milk
    JI Min;ZHANG Meng-hua;LU Dong-lin;JIANG Ping;XU Min;HUANG Xi-xia
    2017, 54(1): 179-183. 
    Abstract ( )   PDF (1028KB) ( )  
    [Objective] This paper of Kashi two households donkey milk farmers fresh donkey milk freezing point of detection and watered test,aims to analyze the donkey milk freezing point is able to become the main basis of donkey milk watered.[Method] UL80BC type milk analyzer was used to determine the freezing point of 50 raw donkey milk samples from the two Xinjiang Yuepuhu County dairy farmers.[Result] Freezing point average of donkey milk was-0.563℃,ranging in-0.671--0.530℃.A household eaw donkey milk added with 1; had no significant difference (P > 0.05),and water and 2; had significant difference (P < 0.05),B households fresh donkey milk added with 1; had significant difference (P < 0.05),and 2; of water have significant difference (P < 0.01),the A and B family added with 4;,adding 6; had significant difference (P < 0.01).[Conclusion] Adding water in the fresh donkey milk,the freezing point rises up,and more water added,the higher it will be.But,the milk with 1; of water,the freezing point decreases with the increase of water content.
    Polymorphism Analysis of DMRT3 Gene in Yili Horse
    HE Mei-sheng;YU Xi;GAO Cheng-cheng;CAO Hang;MA Hai-yu;LIU Ling-ling;JIANG Xin;LIU Wu-jun
    2017, 54(1): 184-189. 
    Abstract ( )   PDF (1190KB) ( )  
    [Objective] In this study,the project aims to analyze the association of DMRT3 gene polymorphism with gait trait in trotting type of 103 individuals Yili horse.[Method] Genetic polymorphism of DMRT3 gene was detected by PCR-RFLP and DNA sequencing technology.[Result] The result showed that there was 1 mutation in DMRT3 gene and there were 3 genotypes:CC,CA and AA.The SNP g.22999655 C > A was identified in DMRT3 gene by sequencing,and it caused the premature stop of the encoded acid.Statistical results indicated that the SNP was significantly associated with speed in Yili horse (P < 0.05).[Conclusion] The mutation might be acted as an crucial DNA genetic marker for trotting performance traits selection in Yili horse.
    Establishment of Two-dimensional Gel Electrophoresis Technique for Sheep Serum
    2017, 54(1): 190-196. 
    Abstract ( )   PDF (1238KB) ( )  
    [Objective] To establish two-dimensional gel electrophoresis methods (2-DE) for sheep serum.[Method]The experimental conditions of 2-DE were optimized,and various extraction and process methods,different pH IPG strips,different concentration of SDS-PAGE were studied.[Result] The results showed that the 10; tca/ acetone precipitation method was used to deal with the serum protein,pH 4-7 IPG,2.5 mg,18 cm,10; sds-page,and a good 2-DE map was obtained.[Conclusion]This study got a good IPG maps for sheep serum protein.We have successfully established a 2-DE technique for sheep serum proteome,which can provide important technical information for evaluation of transgenic sheep biological safety.