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Genetic Analysis of Growth Traits in Short Season Cotton TIAN feng;FAN Shu-li;WEI Heng-ling;WANG Han-tao;ZHAO Shu-qi;PANG Chao-you;HU Shou-lin;YU Shu-xun 2017, 54(2): 197-205.  Abstract ( )   PDF (1174KB) ( )   [Objective] To study the genetic law of short-season cotton growth period.[Method]Our study utilized four upland cotton cultivars to construct two crosses and analyzed three traits(Flowering time FT,Flowering and boll-setting period FBP,Whole growing period WGP)of growth period using the joint segregation analysis method of mixed major gene plus polygene inheritance model.[Result]The results from phenotypic analysis showed that: the other phenotypic traits were in favor of the early-maturing parent and the partial early-maturing parent,except for the combination of F1 in the blooming stage and F2 sowing-flowering stage.The genetic analysis results showed that: the same traits of the combination I and II were in accordance with the same genetic model,and were controlled by two pairs of major genes.[Conclusion]It can be seen from the heritability of major genes and polygenes,the three phenotypic traits were mainly controlled by the major genes and the minor genes,the heritability of the three phenotypic traits is high,and the additive effect is large.This study could provide a theoretical basis for QTL mapping and molecular marker assisted breeding of cotton early maturing traits.

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Probe into Degradable Plastic Film Mulching during Cotton Safety Period under Drip Irrigation in Northern Xinjiang 2017, 54(2): 206-212.  Abstract ( )   PDF (1407KB) ( )   [Objective] To make clear plastic film mulching cotton safety period and study the effects of different degradation films on soil temperature,moisture and the yield of cotton under drip irrigation.[Method]The experiment set up 5 treatments with 3 repeated times,T1-T4 treatment was for the degradation of plastic film processing,and T5 was for ordinary plastic film processing.[Result]The results showed that: the laying of eighty-fifth days(July 17th)T1 treatment,plastic film was completely degraded and only partially degraded by T2 treatment.However,degradation began after 130 days(August 31)later by T3 treatment.T4 treatment made no degradation at all.The soil temperature was increased in April,May and June between 55.4;-62.6;,14.3;-23.5;and 9.6;-14.2;,by all treatments,in July it did show warming effect;and water retention performance of the membrane was not associated with its thickness and degradation rate,among them,T4 treatment produed the best water retention.Plastic film was laid 85 days ago,the plastic film degradation time was inversely proportional to cotton growing development and yield.And 85 days later,the film was not degraded by T3,T4 and T5 treatment,which showed no significant difference in cotton yield.[Conclusion]In summary,the plastic degradation time 85 days after the cotton flowering period can effectively exert the soil warming effect to achieve the stable production.

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Silencing Effect of Jasmonic Acid Carboxyl Methyl Transferase(JMT)Gene on Physiological Indexes of Cotton Seedlings AN Wen-kai;YAN Xiao-hong;BAO Qiu-juan;ZHANG Fu-chun 2017, 54(2): 213-222.  Abstract ( )   PDF (1497KB) ( )   [Objective] Jasmonic methylester transferase(JMT)can effectively facilitate the production of jasmonic methylester,so that it enhances plant drought resistance.[Method]A 450 bp silence fragment of GhJMT was cloned from cotton XLZ17(Gossypium hirsutum)using PCR amplification,and then constructed into vector pTRV.The recombinant plasmid pTRV-GhJMT was transformed into competent Agrobacterium tumefaciens(GV3101).Cotton seedlings in hydroponic conditions were infected with the transformed A.tumefaciens GV3101.After one month,the growing cotton seedlings were treated with PEG6000 for 24 h.The water content,the molality of osmotic adjustment substances,photosynthetic pigments,antioxidants and malondialdehyde(MDA)as well as enzymes activities in cotton leaves under drought stress were measured.[Result]The results showed that content of soluble sugar,ASA,betaine,proline and SOD were reduced by 42.1;,36.6;,69.4;and 71.6;compared with the pTRV-00,and MDA,O2-and H2O2 were increased 0.43,1.45 and 0.59 times compared with pTRV-GhJMT,respectively.[Conclusion]Our results demonstrate that GhJMT gene silencing can directly affect the physiological and biochemical characteristics of cotton seedlings treated with drought stress and reduce drought resistant ability of cotton.

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Study on the Population Structure and Cluster Analysis of Some Sunflower Inbred Lines in Xinjiang by SSR Markers LIU Sheng-li;DUAN Wei;WANG Peng;LIU Yan-Tao;WANG Pei-zheng 2017, 54(2): 223-233.  Abstract ( )   PDF (1821KB) ( )   [Objective] Sequence Tagged Site(STS)is a short DNA sequence,which appears only once on its biological chromosome and is suitable for a landmark of crop genome.[Method]500 STS-SSR primers of sunflower were synthesized randomly and were used to screen the samples of Xinjiang edible sunflower and oil sunflower inbred lines for finding the polymorphism.[Result]All of them 62 markers showed stable polymorphism among those 136 sunflowers with clear,stable and easy to read characteristics,which accounted for 12.4;of the number of selected primers.136 Xinjiang sunflower inbred populations could be divided into 2 groups according to the change of △K and be clustered into 2 groups at the 0.65 similarity level.In the 73 oil sunflower inbred lines,70;inbred lines were assigned to first groups which meant the oil sunflower inbred lines were mainly classified in the first group.Among 30 maintainer lines of oil sunflower lines only 4 maintainer lines were assigned to second groups,the rest were in group I.In the 43 restorer line of oil sunflower,25 restorer lines were allocated to first large groups,and the rest were allocated to other groups,which indicated that the genetic diversity of restorer line was more abundant than maintainer lines.In the 55 edible sunflower inbred lines,only 21;of them were divided into first groups which indicated that edible sunflower inbred lines belonged to the second and the third groups.[Conclusion]This study showed that the genetic diversity of edible sunflower was greater than that of oil sunflower,and some genes between the edible sunflower and oil sunflower materials were crossed and penetrated.The results of this study have provided theoretical support for the construction of the core collection and the rational utilization of resources in the future.

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Effects of Different Doses of Control Fertilizer for Continuous Cropping Obstacle on Soil Physical Properties and Economic Benefits of Corn Seed JING Peng-cheng;WANG Shu-lin;CHEN Yi-shi;LU Wei-hua;MA Chun-hui 2017, 54(2): 234-242.  Abstract ( )   PDF (1094KB) ( )   [Objective] To study the effects of different doses of control fertilizer for continuous cropping obstacles on soil physical properties and the economic benefits of corn.[Method]Field test method was used in 13 years continuous cropping soil of maize seed.Using field experiment method,in order to control corn continuous cropping obstacle of Fertilizer Design 7 treatments for the gradient were: CK,0.85,1.70,2.55,3.40,4.25 and 5.10 t/hm2.no fertilizer as CK,each treatment was repeated 3 times,randomized block design.[Result]Along with the increase of continuous cropping obstacle control fertilizer from 0.85 t/hm2 to 5.10 t/hm2,compared with the control,the soil total porosity,aggregates,saturated water content were increased by 6.42;,14.63;and 17.91;,and soil bulk density decreased by 11.11;,which was a significant difference(P< 0.01);Maize plant height,stem diameter,growth rate,fresh weight and dry weight on the ground were increased by 26.06;,32.12;,26.05;,48.05;and 48.06;,respectively with significant differences(P < 0.01);Corn kernel number,kernel weight and 100 kernel weight were increased by 52.24;,46.06;and 20.70;with significant differences(P< 0.01);The corn yield was increased by 46.04;with significant difference(P < 0.01);but the increased yield of fertilizer per kilogram for continuous cropping obstacle decreased by 63.64;with the increased amount of the fertilizer.[Conclusion]When the control fertilizer application amount of continuous cropping obstacle was more than 3.40 t/hm2,the negative value in profit of fertilization appeared,from which it is clear that the amount should be better below 3.40 t/hm2.Applying this amount,the theoretical corn yield is probably over 6.91 t/hm2,reaching the best economic benefit.

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Effects of Simulated Transport Vibration on the Texture Quality of Hami Melon at Different Stacking Heights XU Bin;CHE Feng-bin;PAN Yan;ZHANG Ting;ZHANG Hui;WU Ji-lin 2017, 54(2): 243-253.  Abstract ( )   PDF (1553KB) ( )   [Objective] This study is designed to ascertain the effect on texture quality of Hami melon at different stacking heights by simulation of transport vibration in order to provide theoretical basis for refrigerated transport.[Method]Setting three treatment groups: top(9 boxes high),middle(5 boxes high)and bottom(1 box high).The immobile boxes were taken as the CK.The experiment lasted 14 d under 4-6℃ and the vibrational frequency was 7 Hz.Samples were checked every two days and the food texture was analyzed to measure the melon′s physical index,including hardness,adhesiveness,cohesiveness,elasticity,chewiness and elasticity modulus.Compared with CK,the vibration influence on the texture at different stacking heights of Hami melon was analyzed.[Result]The vibration had great influence on the pulp hardness,after continuous vibration 4 d,pulp hardness began to decrease sharply,the top′s dropped to 38.3;of the starting value.According to the degree,the order was: top>middle>bottom;The vibration had obvious influence on the adhesiveness,according to degree,the order was: top>bottom>middle;In the beginning of 4 d,the vibration postponed the decrease of melon′s cohesiveness,after 6 d,the vibration accelerated the decrease of melon′s cohesiveness;The pulp hardness dropped to the initial value of 38.3;.Vibration did not obviously influence the melon′s elasticity.According to the degree,the order was: top>middle>bottom;The vibration had obvious influence on the melon′s tackiness,after vibration for 4 d,each treatment group′s tackiness declined obviously,the bottom had the greatest decline,falling to 20.6;of the initial value;The vibration didn′t influence the melon′s chewiness and elasticity modulus obviously.Analyzing the texture parameters results showed that pulp hardness was positively correlated with chewiness and elasticity modulus(P<0.01),and it was positively correlated with tackiness(P<0.05),but adhesiveness was negatively correlated with elasticity;Cohesiveness was negatively correlated with elasticity;Tackiness was positively correlated with chewiness(P<0.01),and positively correlated with elasticity modulus(P<0.05);Chewiness was positively correlated with elasticity modulus(P<0.05).The result of one-way ANOVA showed that there were significant differences in the vibration influence on hardness,elasticity and chewiness at different stacking heights,and sorted by the impact size,the order was: top>middle> bottom.[Conclusion]Low frequency vibration could accelerate the softening of melon pulp,and this influence appears obvious 4 days later.The vibration has influence on melon′s texture at different stacking heights: top>middle> bottom.

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Study on Pollination Compatibility of Plum (Prunus domestica L.) FENG Bei-bei;WEI Ya-jun;LI Wen-wen;NIU Ying-ying;XU Ye-yong;WANG Ming;YANG Hong-li;LIAO Kang 2017, 54(2): 254-261.  Abstract ( )   PDF (1194KB) ( )   [Objective] To study the cross pollination compatibility of different varieties in plum(Prunus domestica L.)and lay the foundation for the configuration and cross breeding of the European cross pollination varieties.[Method]Determination of Victoria of pollen number,pollen viability,fruit-setting rate and growth status of pollen tube of three Prunus domestica L.cv.Stanley,Prunus domestica L.cv.French and Prunus domestica L.cv.[Result]The pollen amount of single flower was Prunus domestica L.cv.Stanley 5.00 ten thousand grain/flower,Prunus domestica L.cv.French 3.12 ten thousand grain/flower and Prunus domestica L.cv.Victoria 1.45 ten thousand Grain/flower.Pollen viability was more than 20;,belonged to the medium-higher pollen viability;3 various of plum cultivars self-fertilization of fruit-setting rate,Prunus domestica L.cv.Victoria 0.47;,Prunus domestica L.cv.French 5.71;,Prunus domestica L.cv.Stanley 8.14;;3 plum cultivars pollination: the Prunus domestica L.cv.Victoria as the female parent,respectively in the Prunus domestica L.cv.French and Prunus domestica L.cv.Stanley as male parent,the fruit setting rate was respectively 5.88;,7.17;.Prunus domestica L.cv.Stanley,Prunus domestica L.cv.Victoria as female parent and male parent as the fruit setting rate was 12.15;,with Prunus domestica L.cv.French was a combination of male fruit rate was 6.34;.Prunus domestica L.cv.French as the female parent,Prunus domestica L.cv.Stanley and Prunus domestica L.cv.Victoria as male parent pollination,fruit setting rate were 11.49;,14.41;.When the plum and Prunus salicina L.cv.with Meilili as male parent after pollination,the fruit-setting rate was ≤ 0.05;,showing incompatibility of hybridization;the fluorescent display plum varieties mutual pollination showed affinity,self-fertilization was slow or abnormal pollen tube emerged,only a small amount of pollen tube went into the base of the style;the phenomenon of pollen tube bending or apex expansion after the hybridization of Prunus salicina L.cv.with Meilili hybrid appeared.[Conclusion]Prunus domestica L.cv.Stanley,Prunus domestica L.cv.French,Prunus domestica L.cv.Victoria and Prunus salicina L.cv.Meilili belonged to the middle-higher pollen vitality of varieties;plum cultivars self-fertilization of fruit-setting rate was relatively lower,so they need pollination tree;the pollen viability and stigma fluorescence microscopy,Prunus domestica L.cv.French and Prunus domestica L.cv.Stanley as pollination varieties to Prunus domestica L.cv Victoria,Prunus domestica L.cv Victoria as for Prunus domestica L.cv.French pollination varieties,Prunus domestica L.cv.Stanley appropriate pollination varieties Prunus domestica L.cv.Victoria and Prunus domestica L.cv.French;Prunus domestica L.cv.French×Prunus domestica L.cv.Victoria was better pollinators;Prunus salicina L.cv.Meilili was not fitted with the plum pollination.

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Study on the Induction and Proliferation of Somatic Embryogenesis from the Mature and Immature Zygote Embryos of Picea schrenkiana Yiliminuer;LI Hong 2017, 54(2): 262-271.  Abstract ( )   PDF (1553KB) ( )   [Objective] To study the induction and proliferation of somatic embryogenesis from the mature and immature zygote embryos of Picea schrenkiana,which might have great significance for the understanding of the mechanism of embryonic development and the establishment of rapid propagation system.[Method]Immature embryo and mature embryo were used as explants to induce culture in DCR medium with different growth regulator concentrations.The induction rates of zygotic embryos in different growth stages and its proliferation were studied using immature zygotic embryos with endosperm,immature zygotic embryos without endosperm and mature embryos as explants.[Result](1)When DCR medium added with different concentrations of 2,4-D and 6-BA was used to induce mature zygotic embryos,the combination of 20 μM 2,4-D and 5 μM 6-BA was the best and somatic embryogenesis induction rate was the highest,up to 55;.(2)The induction rate of three zygotic embryos of somatic embryogenesis collected in different periods showed that the immature zygotic embryos with endosperm(June)was up to 32.6;,the mature zygotic embryos(September)was up to 60.6;,Immature embryo without endosperm(July)was 83.1;,which indicated that July was the best picking period of P.schrenkiana cones.(3)The somatic embryogenesis in the five different proliferation medium showed the the proliferation rate was significant(P< 0.05).The proliferation results of somatic embryogenesis in different proliferation medium showed that the effect of BM2 and SH were better than the MSG,1/2DCR,DCR,and the comprehensive proliferation effect of SH was the best.The proliferation rate was 4.55 g and the rate was up 600;.)(4)The five different somatic embryogenesis in SH proliferation medium showed that the difference between somatic embryogenesis was not significant(P > 0.05).[Conclusion]This study has established the somatic embryogenesis technology platform of P.schrenkiana for the first time,which has provided a scientific basis for the genetic improvement of germplasm and shortened the breeding cycle of P.Schrenkiana.

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Experimental Research on Grafting Propagation of Amygdalus ledebouriana Schleche ZHANG Chu-jie;LI Peng;TIAN Jia;LUO Shu-ping;LI Jiang 2017, 54(2): 272-280.  Abstract ( )   PDF (1263KB) ( )   [Objective] Based on the observation of the survival rate and growth of Amygdalus ledebouriana Schleche by different grafting methods,this experiment is designed to screen out the best grafting method.[Method]In this experiment,Prunus triloba and Prunus armeniaca were used as rootstocks to analyze the survival rate of scion and the growth of shoots of wild almond by different grafting methods,time and sites.[Result]The results showed that the grafting of Prunus triloba was better than that of Prunus armeniaca;the survival rate of the cut-grafting was higher than that of the cleft grafting,bark grafting,tongue grafting and T-shaped grafting,and the cut-grafting method could be promoted on large area;the most suitable grafting time for wild almond was in spring and early autumn,specifically,in March and mid-August;the highest survival rate occurred when the grafting angle was between 15-30°.[Conclusion]The best grafting for wild almond is to select Prunus triloba as rootstocks to graft in March or mid-August each year using cut-grafting method with the grafting angles between 15-30°.

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Effects of Different Fertilization on the Growth and Soil Nutrient of Fritillaria pallidiflora Schrek MA Hong-hong;CHEN Bao-yan;YANG Tao;CHENG Zheng-ming;NIU Xin-xiang;MA Xin-wang 2017, 54(2): 281-288.  Abstract ( )   PDF (1599KB) ( )   [Objective] To explore the best fertilization mode for Fritillaria pallidiflora Schrek in Yili area of Xinjiang.[Method]By using four factors and three levels orthogonal test design(the microbial fertilizer,phosphate fertilizer,bio organic fertilizer + organic material decomposing agent,refined organic fertilizer + organic material decomposing agent,design level 1,design level 2(2 level ×0.5)and design level 3(2 level ×1.5)a total of 9 orthogonal treatments were employed to determine the growth and soil of Fritillaria.[Result]Plant height,plant fresh weight and bulb fresh weight,soil organic matter,total nitrogen content and soil pH value were different by different fertilization amounts;With the development of Fritillaria,the soil pH under each treatment showed significantly higher value than that of the bud and flowering stages,and the regular pattern of soil organic matter and total nitrogen content of soil was not obvious;under different fertilization treatments,there was no significant difference between Fritillaria plants per 667 m2;in the same microbial fertilizer dosage,the change trend of Fritillaria yield showed no obvious regularity,treatment 8,namely,microbial fertilizer being 86 kg/667 m2,P being 18 kg/667 m2,bio organic fertilizer and organic material rot agent being 16 kg/667 m2,refined organic fertilizer and organic material and corrosion inhibitor being 60 kg/667 m2,produced the highest yield of Fritillaria 381.14 kg/667 m2;In a single microbial fertilizer dosage,the change trend of Fritillaria yield had no obvious regularity.[Conclusion]The microbial fertilizer and phosphate fertilizer as base fertilizer,combined with organic fertilizers and organic material decomposing inoculants as supplement agents,and microbial fertilizer topdressing used during the growth period of ditching can obtain high yield.

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Construction and Self-activation Detection of Bait Vector for Yeast Two-hybrid System of TA gene in Acidovorax citrulli HU Jin-feng;HUANG Cheng-wen;LIU Jun 2017, 54(2): 289-296.  Abstract ( )   PDF (1223KB) ( )   [Objective] This project aims to construct a bait vector for yeast two-hybrid system of TA gene in Acidovorax citrulli in order to lay the foundation for exploring the gene function involved in the pathogenic mechanism.[Method]The TA gene was amplified by PCR and cloned into the vector pGBKT7.The verified pGBKT7-TA was then transformed into the yeast strain AH109.The activities of report genes ADE2,HIS3 and LacZ were used to detect the self activation of pGBKT7-TA,and the toxicity of pGBKT7-TA was investigated in nutrient deficient medium.[Result]The constructed bait vector pGBKT7-TA had neither self-activation nor toxic effect in the yeast strain AH109.[Conclusion]The constructed bait vector can be used for the follow-up work of the yeast two hybrid system,which has laid a foundation for further screening of proteins interacting with TA from the cDNA library of cucumber cotyledons infected by Acidovorax citrulli.

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Difference Comparison of Agronomic Traits of Two Breeding Strains of Maca PAN Jing-hai;DENG Chao-hong;BAI Xiao-shan;LIN Ming;XU Qi-jiang;WANG Dong-mei;ZHU Guang-hui;CHEN You-qiang;LIU Hua-jun;LIAO Qing;LI Cheng-ye 2017, 54(2): 297-303.  Abstract ( )   PDF (1191KB) ( )   [Objective] This study aims to compare differences in agronomic traits of two varieties of red and yellow maca and select strains with good agronomic traits and high quality for grain harvest in the hope of providing a theoretical basis for the varieties breeding of maca in our region.[Method]Using randomized block contrast experiment.[Result]Each growth period of red maca was 5 to 7 days ahead of the yellow maca.Red maca was better than brown one in such five test items as basal leaf number,leaf length,plant height,main stem height and the number of sprouts.Crown diameter of red maca was about 1 cm smaller than that of yellow maca,but grain number per plant of red maca was more than that of yellow maca and kernel weight per plant and 1000-grain weight were fewer than those of yellow maca.[Conclusion]Through the comparison of maca aerial parts,red Maca agronomic character is superior to the yellow maca.Yellow maca has short growth period and big full bright color grains.In the aspect of maca seed characters,1000-grain weight of yellow maca is heavier than that of red maca;There is no significant difference in seed number and grain weight per plant.

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Biotype Identification of Bemisia tabaci and 11 Kinds of Common Pesticides Resistance Status in Xinjiang Main Agricultural Areas 2017, 54(2): 304-312.  Abstract ( )   PDF (1126KB) ( )   [Objective] This project aims to make clear the biotype distribution of Bemisia tabaci in the main agricultural areas of Xinjiang and commonly used pesticides resistance level in order to provide a scientific basis for scientific prevention and pesticides resistance management.[Method]Mitochondrial cytochrome oxidase I gene(mtCOI)enzyme sequence amplification polymorphism(CAPs)technology was applied to identify the whiteflies biotype.The resistances to 11 commonly used insecticides in Yining,Hotan,Shache,Turpan and Changji were determined by the methods of leaf-dipping and egg impregnation leaf-dipping.[Result]Biological identification showed that Q type was distributed in the 5 tested field populations.Bemisia tabaci in Hotan was a mixed type with B dominant species.Those found in Yining,Shache,Changji and Turpan belonged to type Q.Resistance monitoring showed that the high level of resistance to imidacloprid resistant population in Turpan reached 57.67 times.The population of Yining was only resistant to Imidacloprid.Those populations in Turpan,Shache,Hotan and Changji resulted in from low to high levels of resistance to imidacloprid,thiamethoxam,acetamiprid and dinotefuran(RR was 5.53-57.67 times)and led to from low to middle levels of resistance to pymetrozine+isoprocarb(RR 5.14-31.92 times);Populations in Turpan and Changji displayed moderate level resistance to avermectins and cyantraniliprole(RR 31.67-32.73 times,10.93-29.57 times)and to low to moderate level resistance to sulfoxaflor(RR 9.7-11.66 times).Shache,Yining and Turpan populations exhibited low to moderate level resistance to pyriproxyfen(RR7.96-31.33 times).[Conclusion]The tested five Bemisia tabaci field populations' biotypes mainly belong to Q biotype with a small amount of B biotype.Different Bemisia tabaci biotype shows different resistance levels,among which,the highest level of resistance is to the Imidacloprid.Resistance management should be carried out according to the local resistance level to pesticides.

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Cloning and Expression Profiling of Odorant Binding Protein Gene in Carpomya vesuviana (Costa) (Diptera: Tephritidae) LI Ya-wei;ZHANG Xiao-ju;WANG Ke-ke;YU Yan-xue;ZHANG Xiang-lin;ZHANG Wei;ZHANG Jun-hua;YANG Ding;CHEN Nai-zhong 2017, 54(2): 313-319.  Abstract ( )   PDF (1486KB) ( )   [Objective] This study aims to clone Carpomya vesuviana Costa odorant binding protein(CvcoOBP)and analyze expression profile of CvcoOBP in order to lay the foundation of researching CvcoOBP in Carpomya vesuviana Costa.[Method]Based on transcriptome sequencing of cephalic RNA,the study has analyzed CvcoOBP according to alignment and phylogenetic analysis.The research was also conducted into the tissue specific expression of CvcoOBP by semi-quantitative reverse transcription and polymerase chain reaction(sqRT-PCR).[Result]The study has obtained seven CvcoOBP that had been named respectively as CvcoOBP1-7(GenBank accession number: KU975053,KU975054,KU975055,KX059394,KU975056,KX059395 and KX059396).According to amino acid alignment,CvcoOBP1,CvcoOBP3,CvcoOBP4,CvcoOBP6 and CvcoOBP7 had been proved to be a family of classic OBP,which contained six highly conversed cysteine residues.CvcoOBP2 and CvcoOBP5 belonged to a family of "Minus-C",which had only four highly conversed cysteine residues.Phylogenetic analysis showed that CvcoOBP1-7 had close relationships with different OBPs.Finally,sqRT-PCR revealed that CvcoOBP1-7 could be highly expressed in abdomen,head and thorax.CvcoOBP1 and CvcoOBP2 could be also expressed in wings with relatively low expression.CvcoOBP1,CvcoOBP5 and CvcoOBP6 were also found in legs.[Conclusion]For the first time,the study obtained and analyzed seven CvcoOBP.Different CvcoOBP are various in terms of expression sites and expression quantity.The CvcoOBP1-6 separately belongs to OBP56g,OBP8a,OBP83a,OBP56h,OBP99c and OBP83ef type.

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Study on Inhibition of Two Kinds of Herbicide for Processing Tomato and Broomrape Seed Germination and Control Effect HE Wei;YANG Hua;XU Jian-jun;SUN Xiao-jun 2017, 54(2): 320-326.  Abstract ( )   PDF (1064KB) ( )   [Objective] To understand the effect of pendimethalin,prometryn and two kinds of medicament mixture to processing tomatoes and broomrape seed germination,processing tomato growth.[Method]The seed treatment and pot experiment were used in the study.[Result]Seed treatment test results showed that germination inhibition rate of 33;pendimethalin oil 7 μg/mL,50;prometryn wettable powder 7 μg/mL and two kinds of medicament mixture 1 μg/mL to broomrape seed were high and the tomato seed germination inhibition rate was low;Pot experiment results showed that inhibition effect of 33;pendimethalin oil 10 μg/mL,50;prometryn wettable powder 7 μg/mL and two kinds of medicament mixture 7 μg/mL to broomrape unearthed and growth was remarkable,and there was small or no inhibition to tomato plants growth.[Conclusion]33;pendimethalin oil,50;prometryn wettable powder and two kinds of medicament mixture had the potential to control broomrape in processing tomato field.

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Species Identification of Parasitic Weed Cuscuta in Shihezi Area Aynigul Yiming;FU Chao;GUO Kai-fa;HE Li;ZHAO Si-feng 2017, 54(2): 327-335.  Abstract ( )   PDF (1458KB) ( )   [Objective] In order to identify the species of Cuscuta in Shihezi area and its surroundings in the hope of providing the basis for scientific control and monitoring of Cuscuta.[Method]Stems,flowers and mature,plump seeds of Cuscuta were collected from different host species in Shihezi area and its surrounding and morphology characteristics and ITS,rbcL,trnL-Fgene sequences were used to identify Cuscuta.[Result]A total of 37 samples of Cuscuta were identified as two Cuscuta species,Cuscuta campestris and Cuscuta monogyna based on the result of morphology and ITS,rbcL,trnL-Fgene sequences analysis.[Conclusion]The main Cuscuta species in Shihezi are Cuscuta campestris and Cuscuta monogyna.

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Effects of Temperature-increasing Water Irrigation on Soil Nutrients and Soil Microbial Biomass in Cotton Rhizosphere MENG A-jing;YAN-Jing;Kudereti Bawudon;GU Cheng-gang;WANG Zhi-guo;FU Yan-bo;RAO Xiao-juan;SHENG Jian-dong;FENG Yao-zu 2017, 54(2): 336-342.  Abstract ( )   PDF (1341KB) ( )   [Objective] This project aims to explore the effects of drip irrigation temperature on soil nutrients and soil microbial biomass in cotton rhizosphere by the field experiment of temperature-increasing water irrigation(ZW)and well water irrigation(CK).[Method]This experiment set a water warming irrigation and well irrigation of 2 levels and 3 repeats with drip irrigation under plastic film to research the content of cotton rhizosphere soil available N,available P,available K and organic matter during cotton bud stage and boll stage,and collected the rhizosphere fresh soil within the depths of 0-20 cm to detect soil bacteria,fungi,actinomycetes number in complete irrigation cycle during the bud stage.[Result]The result showed that in the bud stage,soil available N content by ZW treatment was reduced by 13.51;(P<0.05)than the CK,in the boll stage,soil available P was reduced by 26.7;(P<0.05),the content of available K and organic matter were decreased slightly in both stages(P>0.05).The number of soil microorganisms by ZW treatment was increased during the single irrigation period.Three days after irrigation,bacteria,fungi and actinomycetes were increased by 41.5;(P< 0.05),45;(P< 0.01),19;(P < 0.05).Five days after irrigation,the number of actinomycetes was increased by 93.2;(P < 0.01).Total increase order was bacteria > actinomycetes > fungi.[Conclusion]Water warming irrigation can not only promote the absorption of soil nutrients in cotton,thus reducing the nutrient content in soil,but also enrich the amount of microorganism in soil and accelerate soil nutrient cycling.

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Effects Induced by Inputting Biochar into the Saliferous Gray Desert Soil on the Soil Moisture Movement LIU Yi;HUANG Jian;MA Yan-ru;QI Tong;FENG Yao-zu;MENG A-jin;WANG Xin-yong 2017, 54(2): 343-351.  Abstract ( )   PDF (1497KB) ( )   [Objective] To study the effect of biomass carbon input on soil moisture vertical movement in chloride-sulfate saliferous gray desert soil.[Method]The study materials were gray desert soil in three salinization levels which level 1 was low salinization,level 2 was medium salinization and level 3 was high salinization.Under different percentages of biochar inputs before and through the dropwise add of ddH2O,the time-varying of wetting front and accumulative infiltration capacity were calculated and analyzed.[Result]The vertical migration distance and the cumulative infiltration rate of wetting front were significantly increased(P < 0.05)with the infiltration time of different biomass carbon inputs in each degree of saline soil.The soil moisture movement in level 2 and level 3 saliferous soil were hindered by the biomass carbon;when the input percentage of biomass carbon was less than 4;to level 1 saliferous soil,the soil moisture movement could be facilitated by the biomass carbon;The relationship of biomass carbon input percentage with accumulative infiltration capacity in these three salinization soil were negatively correlated;there was no found in the relationship of biomass carbon inputs percentage with the accumulative infiltration capacity of vertical depth of wetting front in different salinization soil.[Conclusion]The ratio of biomass carbon input,the degree of salinization and the interaction effect of the two have a significant impact on the infiltration efficiency and cumulative infiltration of soil water.The infiltration rate and infiltration amount to moderately saline soil were prominent.Low level input has a promoting effect on water transport in mildly saline soil and inhibitory effect on both cumulative infiltration rate and infiltration rate in severe saline soil.

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Effects of Different Primers and Annealing Tempearatures on the Amplification Eefficiency of Real-Time PCR Reference Gene β-actin in the Halophyte Halostachys Caspica BAI Xue-qin;YANG Rui-rui;ZENG You-ling 2017, 54(2): 352-360.  Abstract ( )   PDF (1496KB) ( )   [Objective] To study the effects of different primers and annealing temperatures on real quantitative PCR amplification efficiency of reference gene β-actin with the halophyte Halostachys caspica as research material.[Method]Standard curves of β-actin and peroxidase gene,POD(representative gene responding to salt stress)from this species were built with well-designed two pairs of primers named β-actin1 and β-actin2 for β-actin as internal reference gene,and a pair of primers for POD gene and amplification curves were obtained under different conditions for these genes.[Result]Results showed that the amplification efficiency of the primers β-actin1 was higher than that of the primers β-actin2 in the Halostachys caspica branches under the 55℃ or 58℃ annealing temperature,and amplification efficiency at 55℃ annealing temperature was higher than that at 58℃.On the other hand,it was found there were some differences in the relative expression levels of Halostachys caspica POD gene using the internal reference gene β-actin with two pairs of primers β-actin1 and β-actin2 under the 55℃ annealing temperature.[Conclusion]The research indicates that primers and annealing temperatures can influence the fluorescence quantitative PCR amplification efficiency and then affect relative expression level of candidate genes at a certain extend.

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Cloning and Expression Analysis of HcDNA pol λ Gene from Halostachys caspica under Salt Stress ZHANG Ji;DU Chi;ZHANG Fu-chun 2017, 54(2): 361-370.  Abstract ( )   PDF (2031KB) ( )   [Objective] The expression of DNA damage repair gene under salt stress is helpful to reveal the relationship between DNA damage repair and salt tolerance of halophyte.[Method]According to the transcriptome of Halostachys caspica under salt stress,a DNA damage repair gene DNA polymerase lambda(HcDNA pol λ)was cloned from Halostachys caspica by RACE.[Result]Sequence analysis indicated that HcDNA pol λ contained an open reading frame of 1,335 bp,which encodes 444 amino acids.Conserved domain analysis showed that HcDNA pol λ was the number of DNA polymerase X family,and phylogenetic tree analysis indicated that HcDNA pol λ was an independent branch,which was an stable hydrophilic proteins sub-celled nuclear.Real time fluorescent quantitative PCR analysis showed that the expression of HcDNA pol λ of assimilating branches gradually up-regulated with the increase of NaCl concentration.At 300 mmol/L NaCl treatments,the expression levels of HcDNA pol λ were increased 3 fold and 5 fold for 7 days and for 14 days,respectively.Finally,at 700 mmol/L NaCl treatment for 14 days,the expression levels of HcDNA pol λ increased 20 fold and reached the peak.[Conclusion]The expression of HcDNA pol λ gene could be induced by salt stress.

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Effects of Clay Mineral Composition on Vertic Characteristics in the Northern Slope of Tianshan Mountain ZHANG Wen-tai;ZHANG Pei-pei;WU Hong-qi;JIA Hong-tao;WEI Ying 2017, 54(2): 371-376.  Abstract ( )   PDF (1239KB) ( )   [Objective] To investigate whether there are vertic soils existing in the northern slope of Tianshan Mountain and explore the internal mineralogical mechanism of vertic soils in Xinjiang by measuring clay mineral composition.[Method]Eleven soil profiles in the northern slope of Tianshan Mountain were studied using traditional field survey approaches.Development degree of soil vertic characteristics was determined according to diagnostic criteria such as cracks,slickenside and wedge-shaped soil aggregates,coefficient of linear extensibility(COLE)and smectite content in clay mineral of 28 soil layers were measured,and their correlation was discussed.[Result]Among the eleven studied soil profiles,four could be relatively classified as soils with strongly developed vertic characteristics.The mean smectite content of the 28 soil layers was 38;.Smectite contents of soils with strongly developed vertic characteristics were significantly higher than those of soils with slightly developed vertic characteristics.A significant power relationship existed between smectite content and COLE.[Conclusion]Low smectite content is the internal mechanism that determines the low development degree of soil vertic characteristics in this study.Distribution area of parent material with high smectite content should be especially considered when surveying vertisols in Xinjiang in the future.

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Comparative Analysis of Amino Acids and Nutrition in the Muscle of Five Kinds of Wild Fish in the Irtysh River WEI Dong-mei;Alimujiang Abudula;SHEN hui;WANG Yong-xing 2017, 54(2): 377-385.  Abstract ( )   PDF (1150KB) ( )   [Objective] To determine the contents of 18 kinds of amino acids in muscle of Tinca tinca,Esox lucius,Perca fluviatilis,Thymallus arcticus and Lota lota living in the Irtysh River and evaluate the nutritional quality.[Method]Using acid hydrolysis and alkaline hydrolysis.[Result]Their total amino acids composition vary from 82.65;to 59.37,of which Thymallus arcticus was lower than the others signally;Mass fraction of essential amino acids varied from 53.52;to 39.95;,except Tinca tinca and Esox lucius,the other three species showed a remarkable difference;Delicious amino acids composition varied from 34.99;to 26.37;,of which Thymallus arcticus and Tinca tinca were lower than the others notably.Compared with the ideal pattern of WHO/FAO,they showed a fine fitness of the essential amino acids composition in the five fishes.RC of the five fishes was 93.27;-83.33;.[Conclusion]These five kinds of fish contain rich amino acids and a balanced essential amino acids proportion,and they also have a high proportion of delicious amino acids.The nutritional value order of the five kinds of fish is: Tinca tinca>Esox lucius>Erca fluviatilis>Thymallus arcticus>Lota lota evaluated by the ratio of amino acid to the reference point and in the meanwhile they reach the specification of commercial fish.

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Cloning, Prokaryotic Expression and Protein Identification of eIF3h Gene from Ovis aries YU Chang-jiang;QI Cheng-nian;ZHANG Yun-sheng;SHEN Ming;YANG Hua;YANG Yong-lin 2017, 54(2): 386-392.  Abstract ( )   PDF (1331KB) ( )   [Objective] To clone gene of ovis aries eukaryotic translation initiation factor 3 subunit H and construct its prokaryotic expression vector,induce the expression and identify target protein in vitro.[Method]Firstly,the ovis aries gene of eIF3h would be cloned from Ovis aries cDNA library using the primers based on eIF3h sequence.The prokaryotic expression vectors were transformed into expression host strain BL21,then after,IPTG was added to induce expression.SDS-PAGE and Western blotting assays were used to detect the expression of target protein.[Result]It successfully cloned eIF3h gene sequences of CDS.The length of the CDS was 1,059 bp.The Escherichia coli containing recombinant vector expressed inclusion body proteins of 40kD after induction by IPTG at 37°C.[Conclusion]The construction of recombinant plasmid is successful,which has provided the basis for further research of eIF3h molecule.