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Effects of Water Stress and Nitrogen Application on Growth and Physiology of Coreopsis tinctoria Seedlings
LI Zhiyuan, JIANG Hong, WANG Yanan, QIN Yanan, YU Ting, ZHANG Pu, DENG Shuangshuang, CHU Jiayao, LI Zhihui, Maierdan Maimaiti, QIN Yong
2020, 57(1):
127-138.
DOI: 10.6048/j.issn.1001-4330.2020.01.015
【Objective】 The main planting area of Coreopsis tinctoria(Coreopsis tinctoria nutt.) is located in the arid and semi-arid climate area of Xinjiang. The physiological response of Coreopsis tinctoria seedlings to drought stress under nitrogen application was studied in order to provide theoretical basis for scientific cultivation of Coreopsis tinctoria in arid areas of Xinjiang.【Method】 Coreopsis tinctoria seeds were collected from Keliyang Township, Pishan County, Hotan area. The growth and physiological response under conditions of no nitrogen application (N0∶0 g/kg soil), low nitrogen (N1:0.1 g/kg soil), high nitrogen (N2:0.3 g/kg soil) and severe water shortage (D1:25%±5%) , mild water shortage (D2: 50%±5%) and normal irrigation (D3:75%±5%) at seedling stage (8 leaves and 1 heart) were studied.【Result】 With the decrease of water content, the plant height, total biomass, root length, root-shoot ratio and fresh weight of above-ground and underground parts of Coreopsis tinctoria decreased. Chlorophyll content, net photosynthetic rate (Pn), transpiration rate (Tr), stomatal conductivity (Gs), intercellular CO2 concentration II quantum yield (φPSII), PS II maximum photochemical efficiency (Fv/Fm), electron transfer efficiency (ETR), photochemical quenching coefficient (qp) continued to decrease, while chlorophyll initial fluorescence(Fo), non-photochemical quenching coefficient (NPQ), malondialdehyde (MDA) content, proline content, The content of soluble sugar and the activity of antioxidant enzyme increased. Low nitrogen only promoted seedling growth under normal irrigation conditions, but low nitrogen did not play an important role in seedling growth under soil water shortage. High nitrogen could increase plant height growth, total biomass, root length, shoot fresh weight, root-shoot ratio, chlorophyll content and Pn, Tr, Gs, Ci,φPSII, Fv/Fm, qp, ETR, under different water conditions to decrease Fo, NPQ, MDA, proline content and soluble sugar content. Especially under severe water shortage, high nitrogen treatment had the greatest effect on reducing antioxidant enzyme activity and MDA content. 【Conclusion】 Mastering the appropriate nitrogen supply level can reduce the negative effect of water stress on Coreopsis tinctoria seedlings to a certain extent, which might be helpful for Coreopsis tinctoria to survive the frail seedling growth period.
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