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    Crop Genetics and Breeding · Cultivation Physiology · Germplasm Resources · Physiology and Biochemistry
    Effects of Different Concentrations of NaCl Stress on Seed Embryo Protective Enzyme Activity during Cotton Germination
    ZHANG Hongxia, YONG Xiaoyu, HE Fei, XIA Jun, LI Huiqin, WANG Tangang
    2023, 60(1): 1-10.  DOI: 10.6048/j.issn.1001-4330.2023.01.001
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    【Objective】 To study the effects of different concentrations of NaCl stress on germination characteristics and key enzyme activities of cotton seeds. 【Method】 Xinluzao 45 and Xinluzao 65 were used as the testing materials and different concentrations of NaCl stress were set to determine relative water content, germination potential, germination rate, proline(Pro), soluble protein(SP), superoxide dismutase, (SOD), catalase (CAT), malonaldehyde(MDA). 【Result】 With the prolongation of the salt stress treatment time, the relative water content continued to increase within 0-15 h, gradually flattened from 15-60 h, and the relative water content showed an upward trend at 63 h.The relative water content of Xinluzao 45 seeds under different NaCl concentrations showed that: 0.2% was higher than the control relative water content, and 0.8% relative water content was the lowest; there was no significant difference between the different concentration treatments of Xinluzao 65.Among the varieties, Xinluzao 65 had a higher water absorption rate than Xinluzao 45, and the relative water content was: Xinluzao 65 > Xinluzao 45. 【Conclusion】 Salt stress in a certain range makes cotton seeds improve their salt tolerance by increasing SOD and CAT activities and reducing MDA content, thus reducing the damage of salt stress on seedling emergence.

    Analysis of QTL Mapping for Fiber Quality Traits in Introgression Lines Cotton
    MA Jun, YANG Yanlong, SHI Weijun, WANG Penglong, ZHENG Juyun, GUO Rensong, HU Wenran, YANG Yang
    2023, 60(1): 11-16.  DOI: 10.6048/j.issn.1001-4330.2023.01.002
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    【Objective】 The research laid a foundation for the function research of high quality gene of long-staple cotton and broad genetic basis of upland cotton. 【Methods】 In this study, Xinjiang’s main early-middle-maturing upland cotton variety Xinluzhong 60 was used as the female parent and the high-quality island cotton variety Xinhai 41 was used as the male parent. Then, the BC1F2 population composed of 151 individual plants combined with Xinluzhong 60 as the recurrent parent was used as the mapping population to construct a genetic linkage map. We excavated stable QTLs related to fiber quality to provide a basis for molecular marker-assisted selection breeding. The linkage map was constructed with SSR markers by Join Map 4.0 software. The QTLs for fiber quality properties in BC1F2 populations were mapped by the inclusive composite interval mapping method with Ici Mapping. 【Results】 The map included 52 SSR loci and 14 linkage groups, covering 824 cM and accounting for 18.5% of the cotton genome. And the longest linkage group is 150.3 cM, containing 6 markers, and the shortest is 0.3 cM, containing 2 markers.A QTL related to the average length of the upper half of the fiber was detected, located on chromosome 14. It can be used as a candidate gene for functional research of cotton fiber quality genes in land-sea introgression lines. 【Conclusion】 The polymorphic markers selected in this study can assist early breeding selection.

    Analysis of Quality Traits and Genetic Diversity of 120 Cotton Germplasm Resources
    ZHAO Shuangyin, WANG Weiran, YAN Xuexue, Bimairemu Abuduaihaiti, DONG Jie, Tuerxon Tuerhong, Alip Aierxi
    2023, 60(1): 17-24.  DOI: 10.6048/j.issn.1001-4330.2023.01.003
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    【Objective】 To study the diversity of fiber quality traits of cotton germplasm resources by using field phenotype combined with molecular marker technology, improve the utilization efficiency of cotton germplasm resources and lay the foundation for the research of hybrid parents germplasm resources. 【Methods】 120 introduced cotton germplasm resources were used as research materials, and the field planting method was employed to study the overall status of fiber quality traits and the correlation between shapes and after that, the genetic diversity of germplasm resources was further analyzed by combining molecular marker technology. 【Results】 The fiber lengths of all 120 samples were less than 33 mm, and the Micronaire Values of 31 samples were in the range of A.The minimum of breaking strength was 24.08 and the maximum was 38.4.A total of 95 polymorphic loci were detected, with an average of 5.58 polymorphic loci per primer pair.Cluster analysis and population structure analysis showed that the tested materials could be divided into two groups, and the classification results were similar to their quality traits. 【Conclusion】 The genetic diversity of 120 germplasm resources is abundant; PSP 25523-1, 5-line of Soviet Union cotton, 28 lines of Soviet Union cotton and Ekangmian 7 belong to high quality cotton varieties with high fiber quality, which can be used as high-quality parents to improve Xinjiang cotton germplasm resources.

    Effects of Acidifier Types and Dosage on Cotton Growth
    CUI Lei, MA Tengfei, Halihashi Yibati, WANG Bin, SUN Chen, YANG Jinyu, QIAO Xiaoyan, HUI Guolong, WANG Jinxin
    2023, 60(1): 25-31.  DOI: 10.6048/j.issn.1001-4330.2023.01.004
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    【Objective】 Field experiments were conducted to study the effects of drip application of acidifier on soil pH, available zinc, cotton root system, yield and quality in root zone, so as to screen out suitable acidifier types and dosage for local cotton production. 【Methods】 Three kinds of Acidifiers, ammonium sulfate (S), oxalic acid (C) and citric acid (N), were set as the test control (CK) without acid treatment. The dosage of ammonium sulfate and urea was determined by substituting 30%, 50% and 70% urea N for each top dressing. The same amount of H+ was provided by acidifier solution as the standard, and then the dosage of other Acidifiers was determined. 【Results】 In the vertical direction, soil pH decreased by 0.05-0.24 with the increase of acidifier application rate; in the horizontal direction, soil pH decreased by 2.3%-3.2% with the increase of ammonium sulfate application rate; no matter in the vertical direction or in the horizontal direction, the closer the distance from the emitter or the higher the proportion of ammonium sulfate replacing urea, the higher the content of soil available zinc; when 30%-70% of nitrogen fertilizer was replaced by ammonium sulfate, oxalic acid and lime decreased When citric acid was applied to cotton root zone with the same amount of H+, the dry matter of 0-30 cm root increased significantly compared with CK. The results showed that the dry weight of root system was S3 > C3 ≈ N3 > CK; ammonium sulfate and oxalic acid had the largest accumulation of dry matter in cotton; compared with the control, the application of acidifier increased the single boll weight of cotton, significantly increased the number of bolls per plant, and the cotton yield increased with the increase of the proportion of ammonium sulfate replacing urea; compared with the control, acidifier promoted the increase of fiber length and specific strength, and reduced the uniformity and yield Micronaire value. 【Conclusion】 Among the three Acidifiers, ammonium sulfate is the best acidifier, and the proportion of replacing urea is 70%.

    Screening and Evaluation of Wheat Germination and Seedling Growth Activity by 16 Plant Methanol Extracts
    ZHENG Ruiming, WANG Li, ZHANG Jungao, YAN Rong, LI Jin, LIANG Jing, LEI Bin, ZHOU Xiaoyun
    2023, 60(1): 32-42.  DOI: 10.6048/j.issn.1001-4330.2023.01.005
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    【Objective】 To explore medical plant resources, develop new pesticides, and promote green agricultural production, the regulatory effects of methanol extracts of 16 common medical plants on wheat germination and seedling growth were investigated, and new plant source extracts with good growth regulation activity were screened out and the appropriate concentration range was determined. 【Methods】 16 kinds of agricultural medicinal plant methanol extract were prepared into four different concentrations (10%, 1%, 0.1%, 0.01%) of the solution.Through germination test screening and potting test verification, the leaf length, stem thickness, dry and fresh weight of aboveground parts, endogenous hormone content and photosynthetic characteristics of wheat seedlings were determined, and the plant-derived extracts with the best effect were evaluated by the membership function method and principal component analysis method and after that the appropriate concentration range was determined. 【Results】 The results showed that the germination potential of 0.01%Sophraalopecuroides, Vallisnerianatans and Cuminumcyminum methanol extracts increased by 10.00%, 8.83% and 7.62%, respectively, and the root length increased by 23.44%, 21.88% and 18.75%, respectively.The comprehensive evaluation of seed germination experiments by membership function method was preliminarily screened out, 0.1% Sophraalopecuroides, 0.01% Sophraalopecuroides, 0.01% Cuminumcyminum, 1%Vallisnerianatans, 0.1%Vallisnerianatans, 0.01%Vallisnerianatans, 10%DaturametelL and 0.01%Euphorbia pekinensis extract, and further potting experiments verified that the leaf length of 0.01% Sophraalopecuroides and Vallisnerianatans extract increased by 16.62% and 15.91%, respectively, and the fresh weight of wheat treated with Cuminumcyminum and Sophraalopecuroides extract increased by 14.78% and 5.15%, respectively.The indexes were comprehensively sorted by principal component analysis, and the top three evaluation scores were 0.01%Sophraalopecuroides, 0.01%Vallisnerianatans and 0.01% Cuminumcyminum extract, which had certain growth promotion potential for wheat. 【Conclusion】 The three concentrations of methanol extracts of 0.01% Sophraalopecuroides, 0.01% Vallisnerianatansand 0.01% Cuminumcyminum have good effects, which can regulate the endogenous hormone level of wheat and promote the growth of seedlings.

    Optimal Selection of Water and Fertilizer for Spring Wheat under Drip Irrigation in Gravel Sandy Soil Based on Response Surface Methodology
    ZHAO Jinghua, YANG Tingrui, ZHANG Heng, Hudan Tumaibai, MA Liang, CHEN Kaili
    2023, 60(1): 43-51.  DOI: 10.6048/j.issn.1001-4330.2023.01.006
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    【Objective】 Based on the spring wheat planting environment with gravelly sandy soil in Fuhai County, Altay, Xinjiang.this paper studied the effects of different water and fertilizer treatments on the growth and development of spring wheat under drip irrigation under the condition of gravelly sandy soil., so as to provide guidance for the application of water and fertilizer of wheat in Altay Prefecture. 【Method】 Three irrigation levels of 30 mm (W1), 45 mm (W2) and 60 mm (W3) and three fertilization levels of urea application 0 kg/hm2 (N0), 300 kg/hm2 (N1) and 600 kg/hm2 (N2) were set, and the results were optimized by ANOVA and response surface methodology. 【Result】 The experiment found that under the same irrigation level, spring wheat plant height and dry matter accumulation of N0 treatment were lower than that of N1 and N2 treatments.Among them, the plant height and dry matter accumulation of N0 treatment and N2 treatment were significantly different (P<0.05); Under the same fertilization level, the plant height and dry matter accumulation of spring wheat increased gradually with the increase of irrigation amount.The amount of irrigation has a significant effect on the effective ear number (P<0.05), but has no significant effect on the thousand-grain weight (P>0.05).The amount of fertilization had no significant effect on the thousand-grain weight and effective ear number (P>0.05).Irrigation amount and fertilization amount have a very significant effect on the number of grains per panicle and yield (P<0.01), and the interaction of water and fertilizer only has a very significant effect on yield (P<0.01). 【Conclusion】 Under the applicable conditions of the response surface model, the optimal water and fertilizer conditions optimized by the response surface method are 60 mm irrigation and 388 kg/hm2 fertilization.Under these conditions, the wheat yield is 6,923.1 kg/hm2; analysis of comprehensive test results It was found that the dry matter accumulation, yield composition and yield performance of wheat under W3N1 treatment (irrigation rate 60 mm, fertilization rate 300 kg/hm2) were better, and the final yield was 7,040.93 kg/hm2, so W3N1 was better.Water and fertilizer treatment.

    Effects of Rhizobium Inoculation Mode on Growth and Development of Multiple Soybean
    WU Shu, XU Yue, XU Yaxin, HUANG Xingjun, WU Quanzhong, CHEN Guodong, ZHAI Yunlong
    2023, 60(1): 52-60.  DOI: 10.6048/j.issn.1001-4330.2023.01.007
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    【Objective】 To screen out the best inoculation method and rhizobia suitable for the growth and yield of soybeans in southern Xinjiang. 【Method】 In this experiment, 3 kinds of inoculation methods (seed dressing, seed manure, drip irrigation) and 3 kinds of rhizobia (SMH12,T6, SN7-2) were used to inoculate soybean variety Suinong 35 in the field, the design of crack zone test was adopted, the changes in soybean agronomic traits, photosynthetic characteristics, yield and yield components were measured. 【Result】 Rhizobia inoculated with different inoculation methods promoted the growth and yield of soybeans in southern Xinjiang to different extents.Seed-dressing T6 treatment had the best growth on average node number of main stem of soybean; Drip irrigation T6 treatment had the best effect on average plant height and LAI growth; Drip irrigation SN7-2 treatment promoted the growth of stem diameter, LAD, grain number per plant and pod number per plant best.Seed-dressing SN7-2 treatment had the best effect on Tr, Gs and Pn, grain weight per plant and yield of soybean. 【Conclusion】 Under the experimental conditions, the combination with the best yield effect is seed dressing-SN7-2, which reached 5,946.0 kg/hm2.The combinations with better yield of drip irrigation-T6 is 5,888.6 kg/hm2, seed manure-SN7-2 is 5,461.6 kg/hm2, and seed manure-SMH12 is 5,446.6 kg/hm2.

    Horticultural Special Local Products
    Analysis of Genetic Structure and Construction of Gene Editing Vector of Melon AMS Gene
    CAI Yi, DAI Dongyang, TAN Hai, WANG Di, YANG Fen, WANG Ling, SHENG Yunyan
    2023, 60(1): 61-68.  DOI: 10.6048/j.issn.1001-4330.2023.01.008
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    【Objective】 The construction of gene editing vector is an important step in gene function identification.Therefore, this article aims to study the gene family identification of ABORTED MICROSPORES (AMS), a candidate gene for male sterility in melon, explore CRISPR gene editing technology and construct its knockout vector, which is the function of melon gene, in the hope of providing a basis for AMS functional validation of the male infertility gene's melon. 【Methods】 In this study, bioinformatics technology was used to identify ABORTED MICROSPORES (AMS) family genes, which were candidate genes for male sterility in melon, and analyze their evolutionary relationships, conservative motifs, and physical and chemical properties.And CRISPR/Cas9 gene editing technology was applied to design gRNA primers in the exon region of the melon AMS gene, with the vector pHSN401 as a template to amplify the sgRNA cloning frame, with Bsa I to digest the pHSN401 vector, and DNA recombinase to construct the recombinant vector and transform it into agrobacterium.In addition to that, a single clone was selected for cultivation, and then PCR identification of bacterial liquid carried out. 【Results】 The results showed that the length of the protein encoded by the melon AMS gene ranged from 501-605 aa, the average molecular weight was about 59,351 Da, and the isoelectric point was between 5.04-6.45.Most of the melon AMS genes were located in the nucleus; CRISPR/Cas9 gene editing technology was used to design three target sites in the CDS region of the AMS gene, namely AMS-pHSN401-1, AMS-pHSN401-2 and AMS-pHSN401-3. 【Conclusion】 After analyzing the bioinformatics of muskmelon, the AMS gene is mainly predicted to be expressed in the nucleus, and the muskmelon knockout recombinant vector AMS-pHSN401 is successfully constructed by using CRISPR/Cas9 gene editing technology and transformed into agrobacterium, and the positive clone is obtained through the test.

    Comparison of Phenotypic and Photosynthetic Differences between Saimisu 1 Jujube and Its Budding
    ZHANG Yanfei, JIN Juan, Abudoukayoumu Ayimaiti, JIA Pingping, YANG Lei, FAN Dingyu, ZHOU Long, HAO Qing
    2023, 60(1): 69-78.  DOI: 10.6048/j.issn.1001-4330.2023.01.009
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    【Objective】 To determine the difference of phenotypic characteristics and diurnal variation of photosynthesis between Saimisu 1 and its budding Saimisu 2 under the same cultivation and management conditions in the hope of providing reference basis for bud breeding. 【Method】 In this experiment, the phenotypic characteristics of the two were observed and compared, the photosynthetic indexes of the two were measured by LI-6800 portable photosynthetic instrument, and then the correlation between leaf characteristics and photosynthetic parameters was analyzed. 【Results】 The flowering periods of Saimisu 1 and Saimisu 2 were basically synchronous, and there were significant differences in flower structure.Only the leaf width had significant differences, and the difference between leaf widths was about 8.90 mm.There was no significant difference in other phenotypic characteristics; It was found that the leaves of Saimisu 1 were slender, and the leaves of Saimisu 2 were flat and long.There was a significant difference between the hanging length and hanging thickness of jujube, but there was no significant difference between the amounts of single hanging flower; there were significant differences in photosynthetic indexes, and the diurnal variation curves of photosynthetic indexes of the two were basically identical.The net photosynthetic rate (Pn), transpiration rate (Tr) and stomatal conductance (Gs) of the two were "bimodal" curves.The phenomenon of "photosynthetic lunch break" was obvious, the curves of water use efficiency (WUE) were large "V", and the WUE of Saimisu 1 was higher than that of Saimisu 2; The differences of Pn, Tr, Gs, Ci and WUE between the two varieties were 2.60 respectively μmol /(m2·s), 1.49 mmol /(m2·s), 0.07 mol /(m2·s), 8.04 μmol /(m2·s) and 0.19 μmol /mmol. 【Conclusion】 Through comparison, it was found that the flowers and leaves of Saimisu 2 are larger than those of Saimisu 1, and so is the growth potential of jujube hanging.In addition, the net photosynthetic rate (Pn), transpiration rate (Tr) and stomatal conductance (Gs) of Saimisu 2 are significantly higher than those of Saimisu 1.

    Effects of Different Rootstock Grafting on the Growth, Yield and Quality of Flame Seedless
    DING Xiang, XIAN He, ZHOU Xiaoming, ZHANG Fuchun, HU Xin, PAN Mingqi, ZHONG Haixia, ZENG Bin, WU Xinyu
    2023, 60(1): 79-85.  DOI: 10.6048/j.issn.1001-4330.2023.01.010
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    【Objective】 To study the effects of different rootstocks on the growth, yield and quality of flame-seedless grape and provide a scientific basis for the dominant flame-seedless grafting combination in Xinjiang cultivation. 【Methods】 5 kinds of grafting the scion of 6 a flame seedless grapes 5BB, 11R, 101-14MG, SO4 and the 188-08 were taken as test materials, flame seedless self-roots were used as a control to determine such quality indicators as the fruit grain weight, spike weight, color, soluble solids content, titratable acid during the whole growth cycle in the field development and production. 【Result】 The results showed that the growth, yield and fruit quality of different grafting combinations were significantly or extremely significantly improved compared with the flame seedless self-rooted seedlings.Flame seedless/101-14MG promoted the early ripening of Flame seedless grape and its fruit weight was the largest, the fruit color the best, the soluble solid content and the ratio of solid to acid the highest. 【Conclusion】 The 101-14MG rootstock grafted with flame-seedless grape has the advantages of early maturity, high yield, good coloring and high soluble solid content, and is the best rootstock for flame-seedless grape among the five kinds of rootstock and ear combinations.

    Differential Evaluation of Heat Tolerance of 15 Grape Rootstocks
    HU Jinge, BAI Shijian, CHEN Guang, CAI Junshe
    2023, 60(1): 86-95.  DOI: 10.6048/j.issn.1001-4330.2023.01.011
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    【Objective】 To study the growth of 15 grape stock varieties and the response of leaf chlorophyll fluorescence parameters to high temperature and evaluate the differential of heat resistance by selecting stocks with strong heat resistance in the hope of providing a theoretical basis for grape grafting cultivation. 【Methods】 15 grape rootstocks were used as experimental material,growth and chlorophyll fluorescence parameters were measured under natural high temperature,and the heat resistance was evaluated by membership function method. 【Results】 Leaves of 110R and Fercal were curled,leaves of Shanhe 2 was slightly curled,others were smooth,leaf margin was normal,no spot was found on leaves.Growth rate was decreased with temperature increasing,and at later stage, growth rate tended to be stable.Chlorophyll fluorescence parameters were measured when temperature was stable above 40℃ and order sample optimal segmentation clustering method was used to analyzed chlorophyll fluorescence parameters of grape rootstock,15 rootstocks were divided into 6 categories. 【Conclusion】 Rootstock Shanhe 2 has the strongest heat resistance and 140R is the weakest.

    Effects of Different Cultivation Posture on Chlorophyll Fluorescence Characteristics of Shine-Muscat grape
    YANG Xiang, SU Xuede, LI Pengcheng, LI Ming, GUO Shaojie, ZHANG Jinqiang
    2023, 60(1): 96-104.  DOI: 10.6048/j.issn.1001-4330.2023.01.012
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    【Objective】 To explore the change of chlorophyll fluorescence characteristics in different grape posture and its influence on tree growth and the quality of fruits. 【Methods】 The response to light intensity and energy allocation strategies of bow pergola trellis (Tp), sunny side (Ts) and night side (Tn) of vertical trellis on 6-year-old Shine-Muscat grape were studied. 【Results】 The result showed that: the Fo of Tp was 12.20% and 20.36%, and Fm was 14.57% and 39.27% higher than those of Ts and Tn, respectively, which was significantly different higher than Ts and Tn.The Fv/Fm of Tp and Ts was 19.95% and 17.05%, and Fv/Fo treatment was 3.69% and 3.31% significantly higher than those of Tn.The initial slope α of different cultivation posture of PSⅡwas Tp (0.28) > Ts (0.24) > Tn (0.22), the maximum electron transfer rate ETRmax (73.04) and saturated intensity PARast (1,170.93) were also the highest, the semi saturated intensity Ik was Ts (271.16) > Tp (257.35) > Tn (246.66).ETR first increased and then decreased, Y(Ⅱ) decreased with the increase of PAR, the values of ETR and Y(Ⅱ) were Tp > Ts > Tn.qP and NPQ of bow pergola trellis were also higher than those of vertical trellis, qP value decreased with the increase of PAR.qP value of each treatment was significantly different, Tp treatment had the largest qP value, followed by Tp treatment, and Ts treatment had the smallest value when PAR was less than 285 μmol/(m2 / s).qP value of Tp and Tn treatment had little difference, and Tp treatment had the smallest value when PAR was more than 285 μmol/ (m2/s).NPQ increased with the increase of PAR, it was increased slower when PAR was lower than 190 μmol/(m2 /s).The increase rate of NPQ value in Tp and Ts treatments was significantly increased, while that in Tn treatment was relatively slow, Tp treatment was significantly higher than that in Ts and Tn treatment when PAR was higher than 190μmol/(m2/s).That was to say, compared with vertical trellis, bow pergola trellis improved the chlorophyll fluorescence characteristics, increased Fv/Fo, Fv/Fm, Y(Ⅱ),increased the maximum potential quantum efficiency and actual quantum efficiency of PSⅡ reaction center, increased qP and NPQ.The energy distribution trend of light energy absorbed by Shine-Muscat grapes with different cultivation postures was basically the same,with the increase of PAR, Y (Ⅱ) gradually decreased, Y (NPQ) gradually increased, and Y (NO) showed a relatively stable small decline trend, the distribution ratio of Y (NO) did not change significantly with the increase of light intensity. 【Conclusion】 In a word, the Shine-Muscat grape has strong adaptability to light and bow pergola trellis are more conducive to photosynthesis of the Shine-Muscat grape.

    Analysis on Cultivation Characteristics and Raisin Characteristics of Five Seedless Grape Cultivars in Turpan Region
    XUE Feng, PAN Xubing, WEI Dengpan, LIU Xiuhai, FAN Guoyuan, HU Jinge, ZHAO Ronghua, CAI Junshe, XIE Hui, BAI Shijian
    2023, 60(1): 105-115.  DOI: 10.6048/j.issn.1001-4330.2023.01.013
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    【Objective】 To analyze the differences of cultivation characters, fruit quality and raisin characteristics of five seedless grape cultivars in Turpan in the hope of providing reference for the popularization and utilization of new seedless grape cultivar and the optimization of cultivar structure in the production area. 【Method】 Five seedless grape cultivars (Thompson seedless, Long-berry Thompson seedless, Centennial seedless, Polette, Zitian seedless) were used as materials in Turpan and their phenophase, growth and fruiting habit, fruit quality and raisin characteristics were compared under open field cultivation.Principal component analysis was used to evaluate the comprehensive quality of grape and raisin. 【Result】 The results showed that there were significant differences in phenophase, growth and fruiting habits, fruit quality and raisin characteristics among the five seedless grape cultivars.Thompson seedless, Long-berry Thompson seedless and Centennial seedless were mid-maturation, Zitian seedless belonged to mid-late maturity, and Polette was of late maturing, percentage of bearing shoots of Centennial seedless and Zitian seedless were above 65.00%, percentage of bud burst was only 53.70%, average yield per plant of Thompson seedless was over 35.00 kg, berry weight of Centennial seedless and Zitian seedless were more than 6.00 g, Thompson seedless was only 3.79 g, soluble solid content of Zitian seedless was 19.66%, however other varieties were more than 21%, total acid content of most of Polette was 0.65%, Zitian seedless was only 0.34%, Centennial seedless and Zitian seedless had slightly rose fragrance and light milk fragrance respectively, comprehensive quality ranking of Zitian seedless grapes was in the first place, followed by Centennial seedless, Long-berry Thompson seedless, Polette and Thompson seedless in order.Raisins weight of Polette and Centennial seedless were bigger than others, 1.58 g and 1.44 g respectively, but Thompson seedless was only 0.79 g, soluble solid content of Thompson seedless and Long-berry Thompson seedless were higher than others, 83.33% and 82.84% respectively, total acid content of most of Centennial seedless and Zitian seedless were lower than others, 1.50% and 1.75% respectively, drying rate of Zitian seedless was only 16.90%, very non-plump. 【Conclusion】 Zitian seedless has high and stable yield, but drying rate is very low, raisin characteristics is bad, raisins of Centennial seedless is the best, but high and stable yield are not good.There is still a lack of table and dried grape cultivars, especially those with aroma, large grains and color diversity.

    Comparative Analysis of Fruit Folic Acid Content and ISSR in Different Grape Germplasm Resources
    LIANG Ju, LIU Guohong, WANG Baomin, HE Qiao, Abrake Niazi, GUO Hongmei, LI Xingting, REN Hongsong
    2023, 60(1): 116-126.  DOI: 10.6048/j.issn.1001-4330.2023.01.014
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    【Objective】 To compare the differences of folic acid accumulation in grape fruits from different sources and to evaluate and screen high folic acid germplasm resources by molecular marker technology. 【Methods】 The folic acid content of 22 grape germplasm resources was detected by ELISA; The genomic polymorphism and cluster analysis of 22 grape germplasm resources were carried out by ISSR molecular markers; The detected folic acid content was analyzed with the clustering results. 【Results】 The fruit folic acid content of 22 grape germplasm resources ranged from 1.93 to 46.95 ng/g.Compared with the clustering results, the 22 grape germplasm resources were obviously divided into two groups at the genetic similarity coefficient of 0.621.The average folic acid content of the first category was 35.79 ng/g; The rest belonged to the second category, with an average folate content of 15.71 ng/g.At the genetic similarity coefficient of 0.622, the 21 grape materials were obviously divided into 2 groups.The average folic acid content of the first category was 16.53 ng/g and the average folic acid content of the second category was 12.24 ng/g.At the same time, the analysis of variance showed that the folic acid content of Eurasian species was slightly higher than that of European and American hybrids by 3.998,7 ng/g; Through ISSR molecular marker technology, 9 primers with stable amplification and abundant polymorphism were selected from 30 primers for ISSR amplification of grape.A total of 78 bands were amplified, including 70 polymorphic bands, with a polymorphic percentage of 89.7%.According to the ISSR amplification results, the Jaccard similarity coefficient was analyzed by NTSYSpc2.10e software, and the cluster analysis was carried out by unweighted paired arithmetic mean (UPGMA) to establish the genetic relationship map.The genetic similarity coefficient of 22 grape germplasm resources was between 0.474,4-0.846,2, and the average genetic similarity coefficient was 0.654,5.At the genetic similarity coefficient of 0.621, 22 grape materials were obviously divided into 2 groups.The first category included one Eurasian species (red balado), and the rest was the second category.At the genetic similarity coefficient of 0.622, the 21 grape materials were obviously divided into 2 groups.The first category included 11 Eurasian species (Yanxing seedless, Pinot Grigio, Ruidu Cuixia, Jingmi, Heiqi Seedless, Seedless White, 1124, Jingcui, Ruby, Seedless Purple, Damina), and 6 European and American hybrids (ZA 1, Xiahei, Mihong, Hongbiao Seedless, Luopu Zaosheng and Yuxuan 1).The second category included 4 Eurasian species (Beauty finger, Pink Yadumi, Munag and Rizamat). 【Conclusion】 Through comparing and analyzing the fruit folic acid content and clustering results of different grape germplasm resources, it can be seen that the folic acid content of population 1 is higher than that of population 2.The folic acid content of Eurasian species was slightly higher than that of European and American hybrids.

    Analysis and Comprehensive Evaluation of Nutritional Quality of Different Mulberry Varieties
    DOU Ziwei, YANG Lu, CHENG Ping, ZHANG Zhigang, LI Hong
    2023, 60(1): 127-139.  DOI: 10.6048/j.issn.1001-4330.2023.01.015
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    【Objective】 To select the dominant varieties suitable for popularization in Xinjiang by analyzing the differences of nutritional composition, composition characteristics and comprehensive evaluation results based on quality of different varieties of mulberry so as to provide reference for variety optimization, new variety cultivation and multiple utilization of fruit mulberry resources. 【Methods】 6 farm varieties and 2 introduced varieties in Xinjiang were taken as experimental materials to determine 33 quality indexes, respectively.Correlation analysis, cluster analysis, principal component analysis and orthogonal partial least square-discriminant analysis were used to distinguish the differences of nutritional quality and key characteristic indexes of mulberry.Finally, the varieties with the best quality were selected according to the score of comprehensive evaluation. 【Results】 The basic nutritional components of different mulberries had certain differences and strong correlation, and there were variations in different degrees; all mulberries contained higher rutin and chlorogenic acid, among which the total phenolic acid contents of medicinal mulberry and Taiwan black were higher, 17 single amino acids were detected in 8 kinds of mulberries, and the changes of Asp and Glu were the most drastic and the average content was high.The highest content of TAAs was black mulberry, NEAAs was the main component of mulberry amino acid, followed by MAAs, and the lowest was CEAAs.Different mulberry varieties were divided into 4 groups by cluster analysis, and 11 different quality indexes were obtained by OPLS-DA analysis.The first five principal components were extracted by principal component analysis, and a comprehensive evaluation mathematical model was constructed: Densemble =0.474,8F1+0.219,7F2+0.142,6F3+0.097,2F4+0.065,8F5, and the final comprehensive evaluation score of mulberry could be obtained by calculation. 【Conclusion】 According to the comprehensive evaluation, the top three are black mulberry, black pearl and Taiwan black mulberry, which can be used as the dominant fruit mulberry varieties suitable for popularization in Xinjiang.

    Effects of Different Concentrations of NaCl and NaHCO3 Stress on Diurnal Changes of Photosynthetic Characteristics of Dried Pepper
    XIAO Zhonglin, YAN Huizhuan, GAO Jie, WANG Siyi, ZHANG Xuxu, Ailixire Nijiati
    2023, 60(1): 140-149.  DOI: 10.6048/j.issn.1001-4330.2023.01.016
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    【Objective】 To study the effects of different concentrations of NaCl and NaHCO3 on the photosynthetic characteristics of two dried pepper varieties Hongjiaowang and Chuanjiao 19. 【Methods】 Hongjiaowang and Chuanjiao 19 were used as test materials, NaCl and NaHCO3 solution concentrations as variables, the net photosynthetic rate, transpiration rate, stomatal conductance and intercellular CO2 concentration of pepper leaves at flowering stage were measured by pot irrigation.Correlation analysis and linear regression analysis were used to explore the diurnal variation of photosynthetic characteristics of two dried pepper varieties. 【Results】 Under NaCl and NaHCO3 stress, the net photosynthetic rate, transpiration rate, stomatal conductance and intercellular CO2 concentration of pepper leaves were decreased after 50, 100, 150 and 200 mmol/L NaCl and NaHCO3 were irrigated in the culture substrate, respectively.Under 200 mmol/L Nacl treatment, the lowest daily average net photosynthetic rate of Hongjiaowang was 13.79 μmol/(m2·s), and that of Chuanjiao 19 was 16.85 μmol/(m2·s) under 200 mmol/L NaHCO3 treatment. 【Conclusion】 NaCl and NaHCO3 stress inhibit the photosynthetic characteristics of two dried pepper varieties Hongjiaowang and Chuanjiao 19.

    Effects of Different Compound Substrates on the Growth and Quality of Herba schizonepetae in Pots
    ZHANG Lifang, LI Zhiyuan, QIN Yong
    2023, 60(1): 150-160.  DOI: 10.6048/j.issn.1001-4330.2023.01.017
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    【Objective】 To explore the effects of different compound substrates on the growth and quality of Herba schizonepetae in pot cultivation and select the suitable compound substrates for the plant in pot cultivation. 【Methods】 The experiment used Big leaf Herba schizonepetae as the testing materials by adding a proportion of perlite, vermiculite to the charcoal and coconut bran, studied the physical and chemical properties on the growth and quality of different compound matrix, analyzed the physical and chemical properties of different mixed substrates and its effects on the potted Herba schizonepetae growth and quality by using the method of fuzzy membership function of different distribution matrix of Herba schizonepetae plants for comprehensive analysis. 【Results】 T2 treatment (peat∶ coconut bran =2∶1) had the highest plant height and fresh weight (29.35 cm and 10.42 g, respectively), the highest soluble sugar content and vitamin C content were 2.19 % and 0.25 mg/g, respectively.The average value of the member function was the highest, which was 0.67, and the comprehensive evaluation result was also the highest. 【Conclusion】 Adding a certain proportion of coconut bran in the matrix of potted Herba schizonepetae can partially replace peat, promote its growth and improve the quality.According to the comprehensive comparison and analysis of yield, quality and other indexes of potted Herba schizonepetae, T2 treatment is recommended for the potted Herba schizonepetae.

    Research on Water Harvesting and Afforestation Technology of Rosa laxaon the Front Mountain belt of the Northern Slope of the Tianshan Mountains
    ZHANG Shuai, CHENG Ping, ZHANG Qiwu, WANG Kai, WU Shengli, LI Hong
    2023, 60(1): 161-170.  DOI: 10.6048/j.issn.1001-4330.2023.01.018
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    【Objective】 To study the effects of different land preparation methods and water and moisture conservation measures on the survival rate, growth and physiological and biochemical indexes of R. laxa plantations, and to screen the best comprehensive technical measures for R. laxa plantations in the frontal belt area of the northern slope of Tianshan Mountain. 【Method】 Under two types of land preparation methods, namely horizontal ditch and V-shaped pit, three single water and moisture conservation measures, namely mulching (A), bentonite water retention (B) and xanthate moisture retention (C), and four comprehensive water and moisture conservation measures, namely A+B, A+C, B+C and A+B+C, were set, and the control was no water and moisture conservation measures. We analyzed the effects of different silvicultural technical measures on soil moisture content of silvicultural land and the response of growth index, physiological index and photosynthetic characteristics of R. laxa. 【Results】 The growth and survival rate of R. laxa flowering were not significantly different from those of V-shaped pits (P>0.05), and the soil water content, light and physiological indexes were better than those of V-shaped pits; The soil water content, photosynthetic characteristics, physiological characteristics, growth and survival rate of R. laxa y flowering were significantly different among different water conservation treatments (P<0.05), and the best treatment was A+B+C.The drought resistance of each silvicultural technique was evaluated comprehensively by the subordinate function value method, with the highest score for the A+B+C treatment and the lowest score for the CK treatment in the horizontal ditch. 【Conclusion】 According to the Silvicultural Technical Regulations (GB/T 15776-2016), the survival rate of afforestation in arid and semi-arid areas is 70% (inclusive) or more for qualified, and the best silvicultural techniques for R. laxa were selected as V-pit A+B treatment and V-pit A treatment by combining the principle of minimum cost.

    Plant Protection · Soil Fertilizer · Prataculture · Animal Husbandry Veterinarian
    Identification of Pathogens Causing the Abnormal Appearance of Apples in Alar Based on High-Throughput Sequencing Technology
    LI Chunyan, LIU Fangting, ZHANG Wangbin
    2023, 60(1): 171-177.  DOI: 10.6048/j.issn.1001-4330.2023.01.019
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    【Objective】 To clarify the pathogenic types and severity of the abnormal appearance of Apples in Alar in the hope of providing a theoretical basis for the prevention and treatment of the disease. 【Method】 In Alar in Xinjiang in this study, the occurrence of apple appearance abnormalities was investigated by five-point sampling method in the field, and the types of symptoms in the field were observed and recorded, and the pathogenic viruses were analyzed in molecular biology by high-throughput gene sequencing technology. 【Result】 The investigation showed that the incidence of a disease in the Company 1 of the Sixth regiment was up to 70.45%.There were three types of field symptoms: malformation, rust fruit and coloring abnormality.The high-throughput sequencing results showed that there were 5 types of virus: apple chlorotic leaf spot virus (ACLSV), apple stem pitting virus(ASPV), apple stem grooving virus(ASGV), apple mosaic virus(ApMV) and apple green crinkle virus(AgrCV), and the virus types were different among different varieties. 【Conclusion】 The virus disease of abnormal appearance of apples in Alar is serious, which is widely distributed and occurs with a mixture of various viruses, among which the symptoms caused by a mixture of various viruses are similar to the symptoms of apple scar skin viroid.

    The Quantity and Activity of Biocontrol Bacteria in Cotton Field Soil and the Correlation of Disease Prevention
    WANG Jingyi, ZUO Changgeng, NIU Xinxiang, YANG Hongmei, CHU Min, WANG Ning, LIN Qing, WANG Youwu, LOU Kai, SHI Yingwu
    2023, 60(1): 178-184.  DOI: 10.6048/j.issn.1001-4330.2023.01.020
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    【Objective】 Verticillium dahliae is a soil-borne disease caused by Verticillium dahliae colonizing vascular bundles by infecting roots.Biological control is the most promising control method.Endophytic and rhizosphere bacteria are considered to be the most potential microbial flora in biocontrol bacteria.This project aims to study the changes in the number of biocontrol bacteria and the incidence of cotton Verticillium wilt after antagonistic bacteria is applied to cotton soil, and explore the relationship between the colonization of biocontrol bacteria and disease prevention so as to provide the application of biocontrol bacteria in the control of cotton Verticillium, hoping this theoretical basis will provide technical support for biological control to ensure the quality of agricultural products and safe production. 【Methods】 In this study, 4 cotton plants were selected for pot experiment with endophytic and rhizosphere antagonistic bacteria Bacillus atrophaeus SHZ-24, Bacillus vanillea SMT-24, Bacillu subtilis SHT-15 and Bacillus velezensis BHZ-29, using plate counting and real-time fluorescence quantification quantitative analysis of bacteria and pathogens. 【Results】 The number of 4 antagonistic bacteria in the soil changed in the same trend, and the survival rate at 50 days was SHZ-24, BHZ-29, SMT-24, SHT-15 from high to low, and the 4 antagonistic bacteria could be reduced by treatment.All four antagonistic bacterial treatments could reduce the disease index of cotton seedlings. 【Conclusion】 BHZ-29 worked better. The colonization of the 4 antagonistic bacteria in the soil is significantly positively correlated with the disease prevention effect.

    Effects of the Amount of Phosphate Fertilizer on Processing Tomato Yield and P Use Efficiency by Drip Irrigation
    LU Dawei, Halihashi Yibat, LI Qingjun
    2023, 60(1): 185-191.  DOI: 10.6048/j.issn.1001-4330.2023.01.021
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    【Objective】 To study the effects of applying different amounts of phosphate fertilizer on the yield. 【Method】 Phosphorus utilization efficiency of tomato under drip irrigation using Jinfan 1606 processed tomato as a test variety through field experiments.Based on the same nitrogen and potassium (N 240 kg/hm2, K2O 90 kg/hm2), five treatments were set up in the test: (1) P0: no phosphate fertilizer was applied; (2) P60: phosphate fertilizer (P2O5) was used at 60 kg/hm2; (3) P120: phosphate fertilizer (P2O5) is 120 kg/hm2; (4) P180: phosphate fertilizer (P2O5) is 180 kg/hm2; (5) P240: phosphate fertilizer (P2O5) is 240 kg/hm2. 【Result】 The results showed that compared with the treatment without phosphorus fertilizer, the application of phosphorus fertilizer significantly increased the phosphorus absorption of processed tomatoes, and the P absorption rate increased with the increase of phosphorus application, but the utilization efficiency of phosphate fertilizer decreased with the increase of phosphorus application.At the same time, the application of phosphate fertilizer significantly increased the yield of processed tomatoes, which increased 3.6%-7.4% compared with no phosphate fertilizer.When the amount of phosphate fertilizer was less than 120 kg/hm2, the yield of processing tomatoes increased with the increase of phosphorus application; and when the amount was over than that, the yield no longer increased significantly.The application of phosphate fertilizer had no significant effect on the quality of processing tomatoes. 【Conclusion】 Under this experimental condition, the suitable phosphorus application rate was 129.7 kg/hm2, and the optimal economic yield was 121 t/hm2.

    Effects of Manure Replacing Part Chemical Fertilizers on Soil Nutrient Contents, Cotton Yields and Returns of Fertilizer Inputs
    SHI Xueping, ZHANG Qian, WANG Yan, DONG Ming, QI Hong, FENG Guoyi, SUN Hongchun, WANG Shulin
    2023, 60(1): 192-196.  DOI: 10.6048/j.issn.1001-4330.2023.01.022
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    【Objective】 In the experiment the effects of manure replacing part chemical fertilizers on soil nutrient contents, cotton yield and returns of fertilizer inputs were studied in middle and south of Hebei province where the cotton lands were low fertility, dry and sandy. 【Method】 Adopting randomized block design 5 treatments including CK with no fertilizer, chemical fertilizers, manure replacing 10%, 30% and 50% chemical fertilizers respectively were designed to investigate soil organic matter, P2O5 and K2O cotent, cotton yield traits and returns of fertilizer inputs. 【Result】 After 3 years of located fertilization the soil organic matter contents increased significantly with application of manure, but total N contents of all treatments kept stable.When applying manure the soil P2O5 and K2O cotents decreased with the reduction of chemical fertilizers.The difference of soil P2O5 and K2O cotents between treatments of chemical fertilizer and reducing 10% and 30% chemical fertilizers were not significant, while it decreased significantly when reducing 50% chemical fertilizers.Applying manure with reducing chemical fertilizers affected cotton yields through affecting boll numbers per plant.There were no significant differences for cotton yields between the treatments of chemical fertilizer and reducing 10% and 30% chemical fertilizers, while the cotton yields decreased significantly when reducing 50% chemical fertilizers.The returns of fertilizers input for applying manure with reducing 10% and 30% chemical fertilizers were significantly higher than other treatments. 【Conclusion】 In middle and south of Hebei province where the cotton lands were low fertility, dry and sandy applying manure 1,500 kg/hm2 with reducing 30% chemical fertilizers can increase soil organic matter content, keep cotton yield stable and achieve the highest returns of fertilizer input.

    Balance and Activation of AP in Dry Area Grey Desert Soil under Long-term Fertilization
    WANG Xihe, LIU Hua, FENG Gu, YANG Jinyu, WANG Yanping, HUANG Jian
    2023, 60(1): 197-205.  DOI: 10.6048/j.issn.1001-4330.2023.01.023
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    【Objective】 To investigate the relationship between soil total phosphorus and soil available phosphorus (Olsen-P) and the response to phosphorus budget, that under the long-term different manure management practices in grey desert soil, and investigate the transformation characteristics of phosphorus in grey desert soil, which provides a reference for the scientific application of phosphate fertilizer and oriented cultivation of soil quality. 【Methods】 Taking the long-term positioning experiment of different fertilizer management that started 1989 as the object to analyze the relationship between soil phosphorus activation coefficient (PAC), budget and soil available phosphorus (Olsen-P) response to soil budget. 【Results】 The PAC of soil treated with NP, PK, NPK and NPKS showed a parabolic change with fertilization years, and reached its peak value at the 16th year.The PAC of soil treated with NPKM and 1.5NPKM presented a very significant linear positive correlation with fertilization years (P<0.01), and the annual increase rate was 0.23% and 0.42%, respectively.CK and NK were deficient in soil apparent phosphorus in the current season, and the average phosphorus deficiency was 8.9and 15.5 kg/hm2, respectively.NP, PK, NPK, NPKS quarter average value of a surplus of soil phosphorus were 35.4, 38.9, 32.3 and 25.4 kg/hm2, NPKM, 1.5 NPKM treatment in the surplus value of soil phosphorus were 49.6, 127.8 kg/hm2, annual amount of surplus with the planting fixed number of year was falling, P processing increment and the total available P in soil was significant correlation between profit and loss, every surplus of 100 kg/hm2 phosphorus, Olsen-p concentration increased by 4.02 mg/kg. 【Conclusion】 Fertilization methods and years can significantly affect the PAC and P profit and loss of grey desert soil, and the application of organic fertilizer can significantly increase the PAC and P surplus of soil; There is peak value of PAC and p in soil when only fertilizer is applied or straw applied.

    Effects of Aerated Infiltration Irrigation on Soil Water Infiltration Rate and Water Salt Distribution
    WANG Xuecheng, WANG Zeyu, YAO Baolin, ZHU Zhu, LIU Ran
    2023, 60(1): 206-214.  DOI: 10.6048/j.issn.1001-4330.2023.01.024
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    【Objective】 To save agricultural water in southern Xinjiang and explore the movement law of soil water and salt under aerated infiltration irrigation mode with a view to providing reference for improving saline alkali land in that region. 【Methods】 Through the indoor soil box test, two factors and two levels of complete test were set as follows: adding standard air volume before irrigation (T1), adding standard air volume in the middle section of irrigation (T2), 1.5 times of standard air volume before irrigation (T3), 1.5 times of standard air volume in the middle section of irrigation (T4), and no aeration treatment CK.The irrigation amount was set to 13 L.After irrigation, standing for 24 hours, the infiltration rate of water in soil and the distribution of water and salt in wet body were analyzed. 【Results】 The results showed that the irrigation end time of aerated treatment was earlier than that of non aerated treatment.The irrigation time of T1, T2, T3 and T4 treatments was 8.70%, 28.99%, 31.88% and 43.48% shorter than that of CK treatment respectively, which indicated that the infiltration rate of soil water in aerated treatment was faster than that in CK treatment, and the infiltration rate also increased with the increase of aeration.The average mass moisture content of soil at the horizontal distance of 0 cm and one-dimensional depth of 20-30 cm were 16.2%, 13.31%, 14.61%, 13.07% and 13.21%, respectively.The average mass moisture content of soil was 5.68%, 8.65%, 8.72%, 8.74% and 9.49% respectively at the horizontal distance of 15 cm and one-dimensional depth of 10-35cm from the infiltration irrigation pipe. 【Conclusion】 This indicated that the moisture distribution of aerated treatment was more uniform than that of CK treatment.Compared with CK treatment, aerated treatment had larger range of water infiltration and desalination, and better effect of salt washing.Under the same aeration rate, the effect of aeration in the middle of irrigation was better than that before irrigation.Therefore, it is suggested to adopt the way of aeration in the middle section of irrigation to increase the amount of aeration so as to obtain a larger range of infiltration and desalination.

    Evaluation of the Whole-Plant Corn Silage Quality of Large-Scale Pastures Based on Fuzzy Similarity Priority Ratio Method
    WANG Ting, ZHANG Fanfan, HUANG Hua, YANG Guangwei, CHENG Weiguo, ZHANG Li, MA Chunhui
    2023, 60(1): 215-225.  DOI: 10.6048/j.issn.1001-4330.2023.01.025
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    【Objective】 The purpose of this study is to determine the quality and production level of whole-corn silage in large-scale pasture in northern Xinjiang and analyze the problems in the production of whole-plant corn silage in the hope of providing basis for promoting efficient production in the region. 【Method】 Whole-plant corn silage in 18 pastures of different scales were collected and nutritional quality, fermentation quality, and mycotoxin content analyzed by using the fuzzy similarity priority ratio method to comprehensively evaluate the whole-plant corn silage in each pasture. 【Result】 The overall level of nutrient quality and fermentation quality of each pasture silage reached the national second-level standard, the mycotoxin content of all pastures conformed to the national limit standard.The comprehensive evaluation showed that the top three pastures were: R > I > Q (pastures with a stock of more than 1,000 heads), the bottom three pastures were: N > L > H (pastures with a stock of less than 500 heads). 【Conclusion】 In summary, the overall quality of whole-plant corn silage in large-scale pastures in northern Xinjiang is excellent, but there is a gap between different scales of pastures.Pastures with inventory of more than 1,000 heads are better than pastures with inventory of less than 500 heads; the quality of whole-plant corn silage in each pasture was mainly affected by silage corn varieties, regional climate characteristics and pasture scale.

    Study on Yield and Quality of Forage Rape under Different Models in Northern and Southern Xinjiang
    YANG Jinyu, XU Yongmei, ZHANG Yanhong, ZHOU Bo
    2023, 60(1): 226-233.  DOI: 10.6048/j.issn.1001-4330.2023.01.026
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    【Objective】 To elucidate the biological yield and quality of forage rape under the mode of direct sowing, multiple cropping and different harvest times in the northern and southern Xinjiang. 【Method】 Brassica napus Siyou 2 was taken as the research object, the experiment of direct seeding and multiple cropping was carried out in the souther and northern Xinjiang to analyze the changes of biomass composition and quality index content of forage rape under different modes. 【Result】 The results showed that in the southern Xinjiang replanting mode, the plant height was 1.47 m, the fresh weight was 0.77 kg, the biomass of fresh grass was 7,923 kg / 667m2, the content of crude protein was 20%, the contents of crude fiber and ash were 21% and 14.5% respectively, and the feed quality was good; Under the northern Xinjiang planting mode, the plant height, fresh weight per plant, yield, crude protein content, fiber and ash content of forage rape were 0.91 m, 0.3 kg, 4,978 kg/667m2, 14.9%, 14.1% and 12.5%, respectively, which were beneficial to digestion and absorption, but some nutritional quality indexes were lower than other planting modes.The yield of forage rape was relatively low under the normal planting mode, the first crop fresh grass yield was 1,243 kg / 667m2, the crude fat content was as high as 3.6%, the plant maturity was high, the fresh grass fibrosis degree was high, and the feeding quality was poor.The contents of crude protein, ash and crude fiber in the second crop were 15.9%, 21% and 18.6%, respectively.The yield of fresh grass was 311 kg / 667m2. 【Conclusion】 Based on the analysis of yield and quality, it is recommended that the replanting mode should be adopted in the southern and northern Xinjiang, and the plants should be harvested as early as possible after maturity, and the whole growth period should be 90 days.

    A Comparative Analysis of Milk Nutritional and Functional Components between Junggar Bactrian Camel and Holstein Cow
    SONG Wenwen, CHEN Zhuo, LI Xin, CHEN Lijing, SHU Zhan, LI Ziqian, GUO Xueping, YE Qianwen, MA Xuelian, SU Zhanqiang, YAO Gang
    2023, 60(1): 234-241.  DOI: 10.6048/j.issn.1001-4330.2023.01.027
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    【Objective】 To compare the milk differences between Junggar Bactrian camel and Holstein dairy cow, and to provide a scientific basis for comprehensive understanding of the nutritional value and biological function of camel milk. 【Methods】 Milk composition analyzer, inductively coupled plasma-optical emission spectrometry, gas chromatography, and ELISA were used to detect the main nutrients, physicochemical characteristics, fatty acids and main bioactive substances content of milk. 【Results】 The contents of milk protein, lactose, calcium, ash, dry matter, density, eicosanoic acid, heneicosylic acid, palmitoleic acid and ALA content in the Junggar Bactrian Camel milk were significantly higher than those in the Holstein dairy cow(P < 0.05).However, conductivity, pH value, water, cis-8, 11, 14-eicosatrienoic acid, caproic acid, caprylic acid, capric acid, lauric acid content in the Junggar Bactrian Camel were significantly lower than those in the Holstein dairy cow (P < 0.05).There was no significant difference in bioactive substances detected(P > 0.05). 【Conclusion】 There were many significant differences between Junggar Bactrian camel milk and Holstein dairy cow milk.

    Effects of Bacterial Bran on Maturity Index of Aerobic Composting of Pig Manure and Its Relevant Evaluation Analysis
    LI An, HOU Yuyong, ZHOU Ling
    2023, 60(1): 242-251.  DOI: 10.6048/j.issn.1001-4330.2023.01.028
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    【Objective】 Effects of Bacterial Bran on Maturity Index of Aerobic Composting of Pig Manure and Its Relevant Evaluation Analysis. 【Methods】 The experiment was carried out by adding waste fungus bran to pig manure aerobic compost taken from a large pig farm in Alar City to explore the influence of different proportion of fungus bran on the physicochemical characteristics of pig manure compost and the spectral characteristics of fermented substrateand to compare the two maturity evaluation methods. 【Results】 The results showed that compared with the control group, the addition of fungus bran could accelerate the compost entering a high-temperature period, and the high-temperature period lasted for 12 days. The compost effect of adding 15% fungus bran was the best, and the content of total nitrogen and other nutrients decreased first and then increased.The infrared spectrum analysis of compost samples showed that there were absorption peaks at 3,330 cm-1-3,370 cm-1, 2,924 cm-1, 1,650 cm-1, 1,554 cm-1, 1,410 cm-1, 1,050 cm-1-1,080 cm-1 during compost, the intensityof characteristic peaks in the treatment group increased more than that of the control group.Fuzzy comprehensive evaluation method and grey relational analysis method were used to analyze and evaluate compost maturity. It was found that grey relational analysis method was more suitable for evaluating compost maturity characteristics. 【Conclusion】 It is beneficial to compost pig manure by adding fungus bran and promoting the compost maturity rate.

    Regional Difference and Dynamic Evolution of Agricultural Green Development Level in China
    CHEN Yulan, CHENG Xurui, GUO Jun, SU Wuzheng
    2023, 60(1): 252-260.  DOI: 10.6048/j.issn.1001-4330.2023.01.029
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    【Objective】 To study regional differences and evolution trend of agricultural green development level in China. 【Method】 Based on panel data from 31 provinces in China from 2010 to 2018, Entropy method and Linear Weighting method to measure the level of agricultural green development, and Dagum Gini Coefficient, Kernel Density Estimation and Markov chain analysis methods were used to empirically analyze the regional differences, distribution dynamics and long-term shift trend of China's agricultural green development level. 【Results】 (1) From 2010 to 2018, the difference in the level of agricultural green development in the eastern and central regions increased year by year, with an average annual growth rate of 4.6% and 2.2%, respectively; The difference of agricultural green development level in western region decreased from 0.113 in 2010 to 0.093 in 2018, and the average annual decline rate was 3.In 2018, the difference between east and west is as high as 0.113.(2) The growth rate of agricultural green development in the eastern region was remarkable, and the western region showed strong polarization characteristics.(3) The self-locking probability of agricultural green development level in low level (I), middle level (II), middle and higher level (III) and higher level (IV) increased in turn, which were 64.29%, 71.25%, 84.38% and 97.73%, respectively, and the transition probability to higher level was higher than that of demotion.When the steady state distribution was reached, it would stand at 81.34% higher level (IV) area. 【Conclusion】 The overall differentiation level of agricultural green development in China is increasing year by year, and there is a certain polarization phenomenon; The difference level of agricultural green development between the east, middle and western regions is remarkable, and the phenomenon of polarization increases in turn.With the continuous development of high quality agriculture in China, the level of agricultural green development in China will be steadily distributed to the higher level (IV).