【Objective】 To systematically evaluate the resistance level of Xinjiang maize germplasm resources to dominant pathogens of corn stalk rot in the hope of providing scientific basis for genetic breeding and scientific control of corn stalk rot resistance.【Methods】 The level of resistance to P. inflatum stem rot of 62 maize germplasm resources and 100 maize germplasm resources to Fusarium stem rot were identified and evaluated by field artificial soil embedding method.【Results】 In 2020, 62 maize germplasm resources were identified for resistance to P. inflatum stem rot by field artificial soil embedding method. Seven highly resistant materials were identified, accounting for 11.29%, and 5, 18, 5 and 27 resistant, moderately resistant, susceptible and highly susceptible materials accounted for 8%, 29%, 8% and 24%, respectively. In 2021, 100 maize germplasm resources were artificially inoculated with F. Verticillioides to identify and evaluate its resistance to fusarium stem rot. 14, 16, 37, 15 and 18 highly resistant, moderate resistant, susceptible and highly susceptible materials were screened out, respectively. In addition, the correlation coefficients between plant rate and plant height, ear length and ear diameter were -0.333**, -0.388** and -0.283**, respectively, indicating that maize varieties with high stalk and ear thickness had a good basis for disease resistance. The correlation coefficients between dry ear weight, grain weight, 100 grain weight and the disease plant rate were -0.203*, -0.222*, -0.379**, respectively, showing significant or extremely significant negative correlation, indicating that the disease plant rate had a great influence on the yield index. The analysis of the effect of stem rot on the field characters of different maize varieties showed that the tasseling stage, silking stage and powder dispersing stage of 21KBL054 were less affected and delayed by 2, 2 and 4 days respectively, while 21KBL096 was seriously affected, and the tasseling stage, silking stage and powder dispersing stage were delayed by 13, 12 and 8 days respectively. The average plant height and ear position of 21KBL058 strains were 224.80cm and 83.09cm, respectively, increased by 22.15% and 51.76% compared with the control. The average plant height and ear position of 21KBL045 strains at the same resistance level were 186.50cm and 72.64 cm, respectively, which increased only by -0.40% and 3.04% compared with the control.【Conclusion】 In conclusion, a number of disease-resistant varieties with excellent disease resistance and stable field characters are comprehensively screened, such as 21KBL096, 21KBL030 and 21KBL033.