新疆部分小麦材料淀粉品质基因等位变异的分子鉴定及其分布

Molecular Identification and Distribution of Allelic Variation of Starch Quality Genes in Some Wheat Varieties in Xinjiang

  • 摘要:目的 】研究小麦淀粉品质基因Wx-A1、Wx-B1、Wx-D1基因的等位变异组成与分布。比较新疆当地小麦材料与外引小麦材料淀粉品质之间的差别,为选育和改良小麦品质奠定基础。【 方法 】利用MAG264、BDFL/BRD、MAG269 分子标记,对新疆小麦材料中淀粉品质基因Wx-A1、Wx-B1、Wx-D1的分布情况进行分子标记鉴定。【 结果 】在鉴定169份材料中,在冬麦材料中Wx-A1a、Wx-B1a类型为18.67%,Wx-D1a类型为10.67%;在春麦材料中的Wx-A1b类型占30.85%,Wx-B1b类型占41.49%;国外品种、其他国内品种、新疆当地品种等材料中,含有Wx-A1a基因的比例分别为74.49%、84.78%、56%,含有Wx-A1b基因的比例分别为25.51%、15.22%、44%;含有Wx-B1a基因的比例为63.27 %、82.61 %、64 %;含有Wx-B1b基因的比例为36.73%、17.39 %、36 %, 国外品种和新疆当地品种的全部携带Wx-D1a基因,其他国内品种含Wx-D1a基因为82.61%;携带Wx-D1b突变类型中,其他国内品种为17.39%,国外品种和新疆当地品种无材料携带此基因。【 结论 】94份春小麦的缺失Wx-A1、Wx-B1亚基突变频率远远高于冬小麦,新疆品种材料中未检测出Wx-D1b基因类型。春麦材料Wx-A1a、Wx-B1a基因的比例同为18.67%,Wx-D1a基因的比例为10.67%。同时缺失Wx-A1、Wx-B1基因类型的品种有14份,其中4份为新疆品种,10份为国外品种。同时缺失Wx-B1、Wx-D1基因类型的有3份,都是国内品种。

     

    Abstract:Objective 】 The study is to research the allelic composition and distribution of starch quality gene (Wx-A1,Wx-B1 and Wx-D1) and the difference of starch quality gene between local wheat materials of Xinjiang and introduced wheat materials in the hope of laying the foundation for improving wheat variety in Xinjiang. 【 Method 】Using molecular markers MAG264, BDFL/BRD and MAG269 to identify the distribution of starch quality genes Wx-A1,Wx-B1 and Wx-D1 in cultivated wheat germplasms in Xinjiang.【 Result 】The results showed that in the 169 materials to be identified, 18.67% materials of winter wheat had allele Wx-A1a and Wx-B1a,10.67% had Wx-D1a, and 30.85% materials of spring wheat had allele Wx-A1b, 41.49% had Wx-B1b. The distribution frequencies of oversea cultivars, other Chinese cultivars, Xinjiang local cultivars were respectively 74.49%,84.78% and 56% in mutant gene type Wx-A1a,for Wx-A1b gene type, they were respectively 25.51%,15.22% and 44%, for Wx-B1a gene type they were respectively 63.27 %, 82.61 % and 64 %,for Wx-B1b gene type they were respectively 36.73%,17.39 % and 36 %. All foreign varieties and Xinjiang local varieties carried Wx-D1a gene, 82.61% materials of other Chinese cultivars had Wx-D1a and 17.39% materials of other Chinese cultivars had Wx-D1b. Foreign varieties and local varieties of Xinjiang did not carry this gene.【 Conclusion 】The study concluded that the frequency of subunit mutation of Wx-A1 and Wx-B1 genes in 94 spring wheat was much higher than that in winter wheat and Wx-D1b gene was not tested in Xinjiang local cultivars and other Chinese cultivars. 18.67% materials of spring wheat had allele Wx-A1a and Wx-B1a gene, 10.67% had Wx-D1a. At the same time, 14 varieties lost Wx-A1 and Wx-B1 gene types, among which 4 were Xinjiang local cultivars and 10 varieties were foreign ones. At the same time, three copies were of Wx-B1, Wx-D1 deletion gene type and they were all domestic cultivars.

     

/

返回文章
返回