Abstract: 【 Objective 】 In order to solve the slow Philodendron con-go seedling cuttings, sowing, ramet production problems, this project aims to provide a theoretical basis for the large-scale production by taking advantage of tissue culture technology.【 Method 】The annual stems of Philodendron con-go were employed as experimental materials. The study assessed induction and proliferation medium, simplified rooting, transplanting method and rapid tissue culture for producing high quality seedlings.【 Result 】The results showed that the induction philodendron medium was MS plus BA1.5 mg/L with the addition of NAA0.1 mg/L, and the proliferation medium was MS plus BA 0.2 mg / L plus NAA0.1 mg / L, in which, the survival rate reached 98%. Peat and perlite mixed matrix was the most suitable for transplanting the aerial roots. 【 Conclusion 】Technical research of tissue culture production technology of Philodendron con-go established rapid propagation system and provided reliable technical support for quality seedling factory production.