Abstract: 【 Objective 】 To explore the effect of polysaccharide extracted from traditional Chinese medicines on immunomodulatory in different MHC B Lβ II genotype chickens.【 Method 】PCR-SSCP technique was applied to group feather white feather chickens according to different MHC B-LβII genotype, the different MHC B-LβII genotypes were collected from white feather chickens and peripheral blood lymphocytes were isolated. cCHMPS of the final concentrations 100 μg/mL, 75 μg/mL, 50 μg/mL, 0 μg/mL was added and cultured together for 24 h, and then the expression of lymphocyte IL-2, IL-12, IL-4 mRNA was detected by using Real-time PCR method.【 Result 】(1) Traditional Chinese medicinal polysaccharide extraction of different doses could improve IL-2, IL-12, IL-4 mRNA expression levels of chicken lymphocyte compared with the control group in each MHC B Lβ II genotype chickens (P ＜0.05). (2) And when the traditional Chinese medicines concentration was 50 μ-g/mL, the IL-2, IL-12, IL-4 mRNA expression levels of AA and BC genotype chicken lymphocyte were higher than those of the same genotype. When the traditional Chinese medicines concentration was 100 μg/mL, the IL-2, IL-12, IL-4 mRNA expression levels of BB genotype chicken lymphocyte were higher than those of the same genotype.【 Conclusion 】The traditional Chinese medicinal polysaccharide extraction could stimulate IL-2, IL-12 , IL-4 mRNA expression in different MHC B-LβII genotype chickens, and the optimum immunomodulatory doses vary in each MHC B-LβII genotype chickens.