Abstract: 【 Objective 】 This experiment was conducted to study the effects of intramuscular injection of compound polysaccharides on avian influenza antibody titers in serum of different MHC B-LβII genotype laying hens. 【 Method 】500 chickens were divided into three groups according to different genotypes by PCR-SSCP method. The genotypes were divided into high, medium and low dose Chinese herbal compound polysaccharide groups and blank control group. The test chickens of each group were intramuscularly injected with 50 mg / mL, 25 mg / mL, 12.5 mg / mL Chinese herbal compound polysaccharide and saline, 0.2 mL / day for 7 days. At the age of 27, 35, 42 and 49 days, 5 chickens were randomly selected from each group and vein blood was collected from the wings for separation of serum. The titer of H5 and H9 subtype avian influenza antibodies in serum was measured by microhialysis inhibition test (HI).【 Result 】The results showed that, before immunization, the average titer of H5N1 Re-6 and H9N2 maternal antibody was higher than that of the control group, and the titer of H5N1 maternal antibody in AA and BC genotype of high-dose Chinese herbal compound polysaccharide group was significantly higher than that of control group (P <0.05). On the 7 day after immunization, the titer of H5 subtype avian influenza antibody in AA genotype chickens in high, medium and low dose groups was significantly higher than that in control group (P <0.05), the titer of H5 subtype avian influenza antibody in BC genotype high dose group was significantly higher than that in control group (P <0.05) and the titer of H9 subtype avian influenza antibody in BC high, medium and low dose groups was significantly higher than that in control group (P <0.05); On the 14 days after immunization, the titer of H5 subtype avian influenza antibody of AA genotype high and medium dose group was significantly higher than that of control group (P <0.05), the BB genotype in the middle dose group was significantly higher than that in the control group (P <0.05) and the H9 genotype BC avian influenza in the middle dose group was significantly higher than that in the control group (P <0.05); On the 21 days after immunization, the genotype H5 avian influenza of BC genotype high and medium dose group was significantly higher than that of control group (P <0.05) and the avian influenza of BC genotype chicken high dose group was significantly higher than that of control group (P <0.05).【 Conclusion 】These results indicated that different doses of compound herbal Chinese medicine polysaccharide could improve the titer of H5 and H9 subtype avian influenza antibodies in serum of laying hens to certain degrees, and the medium dose and high dose are of good effect.