Abstract:
【Objective】 In order to understand the cold resistance of two parents and their effects on offspring and provide a theoretical basis for breeding cold resistance of Korla pear hybrids.【Method】Using 3 year old F
1 generation of Korla fragrant pear × Hesejujuli and its parent group as the research object, the electrolyte leakage rate, soluble sugar content, free proline content, MDA content, SOD activity and the recovery germination rate were determined after the annual dormant shoots were treated under different artificial low temperature stress. 【Result】The electrolyte leakage rate, MDA content, soluble sugar content, free proline content of three group annual dormancy branch increased gradually following with the temperature decreased, then remained stable or declined slightly; the SOD activity increased gradually as the temperature decreased, and got the peak at semi lethal temperature (LT
50), and then it goes down rapidly. The branches germination rate decreased gradually following with the temperature decreased, which were no longer germination when the temperature exceeds the -33℃. In the three group branches, the annual dormant branches of male parent Hesejujuli showing strong cold resistance that the electrolytic leakage rate and MDA content were the lowest, and the soluble sugar, free proline content, germination rate and SOD activity were the highest. The cold resistance of the male parent Hesejujuli and of the F
1 generation Korla Fragrant Pear × Hesejujuli shows stronger than that of female parent Korla Fragrant Pear, in which the cold resistance of F
1 generation a little weaker than that of Hesejujuli. 【Conclusion】The male parent with strong cold resistance can greatly improve the cold resistance of the hybrid progeny.