库尔勒香梨×褐色句句梨F1代抗寒性研究

Study on the Cold Resistance of F1 Generation Korla Fragrant Pear × Hesejujuli

  • 摘要: 【目的】研究库尔勒香梨和褐色句句梨两个亲本抗寒性及其对杂交后代的影响,为选育抗寒性强的库尔勒香梨杂交后代提供理论依据。【方法】以3年生库尔勒香梨×褐色句句梨F1代群体和其亲本群体为研究对象,对一年生休眠枝进行人工低温胁迫处理,测定不同胁迫温度下休眠枝的电解质渗出率、可溶性糖、游离脯氨酸含量、丙二醛(MDA)含量、超氧化物歧化酶(SOD)的活性和枝条恢复萌芽率。【结果】母本库尔勒香梨、F1代杂交群体及父本褐色句句梨三个群体一年生休眠枝的半致死温度分别为-24.7、-26.5和-28.6℃。三个群体一年生休眠枝可溶性糖、MDA和游离脯氨酸含量均随温度下降而升高,随后趋于平稳或小幅下降;SOD活性随着温度的下降逐渐升高,在半致死温度附近达到峰值之后迅速下降;库尔勒香梨在低于-30℃后,褐色句句梨和F1代杂交群体在低于-33℃后芽体全部死亡。三个群体中,父本褐色句句梨一年生休眠枝的电解质渗出率、MDA含量最低,可溶性糖、游离脯氨酸含量、萌芽率和SOD活性最高,表现出较强的抗寒性。父本褐色句句梨和库尔勒香梨×褐色句句梨F1代群体一年生休眠枝的抗寒性显著强于母本库尔勒香梨,且F1代群体的抗寒性稍弱于父本褐色句句梨。【结论】抗性较强的父本能够较大的提高杂交后代群体抗寒性。

     

    Abstract: 【Objective】 In order to understand the cold resistance of two parents and their effects on offspring and provide a theoretical basis for breeding cold resistance of Korla pear hybrids.【Method】Using 3 year old F1 generation of Korla fragrant pear × Hesejujuli and its parent group as the research object, the electrolyte leakage rate, soluble sugar content, free proline content, MDA content, SOD activity and the recovery germination rate were determined after the annual dormant shoots were treated under different artificial low temperature stress. 【Result】The electrolyte leakage rate, MDA content, soluble sugar content, free proline content of three group annual dormancy branch increased gradually following with the temperature decreased, then remained stable or declined slightly; the SOD activity increased gradually as the temperature decreased, and got the peak at semi lethal temperature (LT50), and then it goes down rapidly. The branches germination rate decreased gradually following with the temperature decreased, which were no longer germination when the temperature exceeds the -33℃. In the three group branches, the annual dormant branches of male parent Hesejujuli showing strong cold resistance that the electrolytic leakage rate and MDA content were the lowest, and the soluble sugar, free proline content, germination rate and SOD activity were the highest. The cold resistance of the male parent Hesejujuli and of the F1 generation Korla Fragrant Pear × Hesejujuli shows stronger than that of female parent Korla Fragrant Pear, in which the cold resistance of F1 generation a little weaker than that of Hesejujuli. 【Conclusion】The male parent with strong cold resistance can greatly improve the cold resistance of the hybrid progeny.

     

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