Abstract: 【Objective】 In the experiment different P fertilizer amounts were designed to study the effects on soil P₂O₅ content, P uptake in plant organs and cotton yields after 6 years of located fertilization. 【Methods】 8 phosphate fertilizer dosage gradient treatments were adopted by random block design to determine the soil available phosphorus content, cotton leaf, cotton stem and seed phosphorus accumulation and cotton yield after 6 years of located fertilization. 【Results】 After 6 years of located fertilization the P₂O₅ contents in soil layers of 0-20 cm increased from 3.1 mg/kg to 65.4 mg/kg from low gradients to high ones, and the soil P₂O₅ content kept stable when the P₂O₅ fertilizer amount was 90.0 kg/hm². With the increase of P₂O₅ amount P content in leaf increased firstly then kept stable when the P₂O₅ fertilizer amount exceeded 90.0 kg/hm². The P content in straw for CK was significantly lower than the treatments of applying P₂O₅ fertilizer, but the differences between treatments of applying P₂O₅ fertilizer were not significant. The P content in seed cotton increased firstly then leveled with the increase of P₂O₅ fertilizer. The uptake of P₂O₅ in leaf, straw and seed cotton increased firstly then kept stable with the increase of P₂O₅ fertilizer, and the uptake of P₂O₅ in seed cotton varied from 51.8-61.7 kg/hm² in different treatments. Compared with CK the seed cotton yield increased by 4.9%-8.2% in treatments of applying P₂O₅ fertilizer, reaching significant level. and the difference was significant, but there were no differences among treatments of applying different amounts of P₂O₅ fertilizer. 【Conclusion】 In cotton areas of central and southern Hebei Province, the optimum P₂O₅ fertilizer was 60.0-90.0 kg/hm² which could keep the balance of soil P₂O₅ and acquire stable cotton yield.