Abstract: 【Objective】 The high molecular weight wheat gluten subunite (HMW-GS) plays an important role in regulating the quality of wheat processing. This project aims to understand the difference in the composition of HMW-GS of Xinjiang spring wheat and introduced CIMMYT materials in the hope of providing a basis for spring wheat hybrid selection in Xinjiang. 【Method】 SDS-PAGE electrophoresis technology was used to analyze the composition and distribution frequency of HMW-GS in 192 materials of spring wheat in Xinjiang. 【Result】 The results showed that 13 HMW-GS alleles were detected in the test materials, with the highest Null subunit frequency (64.06%) of the Glu-A1 site allele, followed by 1 subunit (21.35%) and 2 subunit (14.58%). The Glu-B1 site allele variant type was the most abundant, with sub-base frequency order of 7 + 8 (39.58%) >7 (17.19%) > 7 + 9 (13.54%) > 17 + 18 (11.46%) >22 (9.38%)> 13+16 (7.29%) > 6+8 (1.04%) > 14+15 (0.52%); The Glu-D1 bit detection frequency of 2 plus 12(49.48%) and 5 plus 10(50.52%) was close. The high-quality sub-base ratio of Glu-A1, Glu-B1 and Glu-D1 sites was shown in the introduction of CIMMYT materials > spring wheat varieties (series) in Xinjiang. 【Conclusion】 All HMW-GS formed a total of 32 sub-base combinations, the average quality score was divided into 7.04 points, of which the new varieties (departments) of Xinjiang spring wheat cultivated in recent years showed a higher quality score of 8.12 points, significantly higher than the introduction of CIMMYT material quality average score of 6.09 points. There were 25 materials scored at 10 and 90 materials scored above 8.