Abstract: 【Objective】 To study the effect of nitrogen fertilizer application ratios on the yield, quality and nitrogen use efficiency of upland rice in the hope of providing a theoretical basis for the rational application of nitrogen fertilizer for upland rice. 【Method】 In this experiment, a plot test was carried out, and four treatments were set, respectively: (1) control (no nitrogen fertilizer was applied); (2) The distribution ratio of nitrogen fertilizer was tiller fertilizer: panicle fertilizer = 2∶8; (3) The distribution ratio of nitrogen fertilizer was tiller fertilizer: panicle fertilizer = 6∶4; (4) The distribution ratio of nitrogen fertilizer was tiller fertilizer: panicle fertilizer = 8∶2; The nitrogen application amounts were all 120 kg/hm², respectively expressed by: CK, N (2∶8), N (6∶4) and N (8∶2). 【Results】 The yield under N (6:4) treatment increased by 17.2% and 13.7%, respectively, compared with that under N (2∶8) and N (8∶2) treatment.Compared with N (2∶8) and N (8∶2) treatments, the yield of N (6∶4) treatment increased by 31.9% and 23.4%, respectively, and the yield of N (6∶4) treatment with partial productivity increased by 17.2% and 13.7%, respectively.In terms of processing quality, nitrogen application significantly reduced the white rice rate and the whole white rice rate, but increased the brown rice rate.The protein content of rice could be significantly increased by increasing the proportion of ear fertilizer, which was beneficial to the nutritional quality of rice. 【Conclusion】 Under the same nitrogen application level, the yield and rice quality of upland rice can be significantly increased and the nitrogen use efficiency of upland rice can be improved when the ratio of nitrogen fertilizer is tiller fertilizer∶panicle fertilizer = 6∶4.