Abstract:
【Objective】 This study aims to optimize flax germination salt tolerance identification system and the comprehensive evaluation of germplasm resources of salt resistance, salt resistance for flax germplasm resources evaluation and salt-tolerant varieties to lay the relevant foundation.
【Method】 This study selects the shoot long, root length and germination rate as flax germination salt resistance identification of phenotypic traits. Firstly, 30 flax germplasm were treated with 13 NaCl concentration gradients, and the stress concentration was determined by analyzing the changes of different indexes. Then, 150 flax germplasm were stressed with the determined concentration, and the relative shoot length, relative root length and relative germination rate were counted respectively. The salt tolerance of tested flax in germination stage was comprehensively evaluated by membership function method, and the salt tolerance grade was divided by cluster analysis.
【Result】 The appropriate NaCl concentration is 100 mmol/L when the relative shoot length or relative root length is taken as the index, and 220 mmol/L when the relative germination rate is taken as the index; the 150 flax germplasm were divided into five salt tolerance grades: 3 high-tolerance, 36 tolerance, 67 moderate-tolerance, 33 sensitivity and 11 high-sensitivity; all indexes of oil flax subgroup were significantly higher than those of fiber flax and dual-purpose flax.
【Conclusion】 The optimum screening concentration of different salt tolerance indexes is different. Three selected flax germplasm with high tolerance can be used for salt tolerance breeding and follow-up research. Oil flax is more salt-tolerant than dual-purpose flax and fiber flax.