Abstract:
【Objective】 The purpose of the research is to screen the growth-promoting bacteria that can induce tomato systemic resistance and verify its control effect against early blight and gray mold of processing tomato, so as to provide theoretical basis and bacterial resources for the biological control of processing tomato diseases in Xinjiang.
【Method】 The soil bacteria were isolated by the dilution coating terile normal saline and NB medium diluted to 10
4 times without inoculation as blank control, the germination experiment was used to initially screen processing tomato growth-promoting bacteria.After irrigating root of tomato seedlings with strain fermentation liquid,clean water treatment as a blank jasmonic acid content in the leaves was measured to re-screen the systemic resistance inducing disease index and induced effect counted after inoculation with the pathogen of tomato early blight and gray mold 16S rDNA sequence was determined to identify the strain initially.
【Results】 A total of 147 strains of bacteria were isolated, and 19 strains were screened to significantly (
P<0.05) promote the germination and root growth of processed tomato seeds, and 4 strains significantly (
P<0.05) increased the content of jasmonic acid in processing tomato,3 strains were obtained, which effectively control early blight and gray mold of processed tomato at the same time with the induced effect between 27.59% and 39.44%.Strain FY10, FY12, and FY93 were preliminarily identified as
Bacillus atrophaeus,
Pseudomonas wadenswilerensis and
Bacillus pumilus.
【Conclusion】 3 growth-promoting bacteria obtained with the ability to induce systemic resistance and effectively control early blight and gray mold of processed tomato.