Abstract: 【Objective】 A strain G. resinaceum LZ02 with high-yield laccase was screened from Tianshan Mountains in Xinjiang. This project aims to optimize its fermentation technology and study its partial enzymatic properties. 【Method】 The single factor experiment was used to optimize the enzymatic production conditions and study the enzymatic characteristics. In the meanwhile, the protein was detected by two dimensional electrophoresis. 【Result】 The optimal carbon was 2.5% wheat bran and 1% glucose, nitrogen sources was 0.5% casein. Adding Cu²⁺, Mg²⁺, Fe²⁺, Zn²⁺ and Ca²⁺ could promote the yield of laccase, the effect of Co²⁺ was adverse. Veratryl and Guaiacol delayed enzyme production peak from the second day to the ninth day, Tannic acidhad inhibiting effect to produce enzyme. The study of enzymatic properties showed optimal reaction temperature of laccase was 60℃ and there was still activity at 80℃, optimal reaction pH was 3.0 and enzyme activity was stable when pH was 4.0-5.0 and temperature was under 40℃. In addition, Co²⁺, Cd²⁺, Cr²⁺and Pb²⁺ could damage enzyme stability. Bidirectional electrophoresis was performed on the bacteria and fermentation liquid in which isoelectric point of laccase was determined to be 4.1. A pure enzyme protein sequence was obtained by mass spectrometry that showed the protein to be Laccase (Ganoderma Lucidum). 【Conclusion】 The synthesis and secretion of laccase from G. sessinaceum are strictly regulated by nutrient level, culture condition, growth stage and inducers in the medium. Lignin or lignin-related aromatic compounds, N and C sources can also regulate the synthesis of laccase. The response of laccase activity to different kinds and concentrations metal ions and inducers is different, indicating that laccase activity has a more complex physiological function and regulatory mechanism.