Abstract: 【Objective】 To study the effects of different delinting modes on cotton seed vigor and seedling characteristics under PEG stress in the hope of providing theoretical support for producing cotton seeds with high vigor. 【Methods】 Xinluzao 64 was used as the experimental material and three delinting treatments including T₁ (sulfuric acid), T₂ (laser) and T₃ (laser) were set.Then standard germination test was carried out under four modes CK (0%), P₅ (5%), P₁₅ (15%) and P₂₅ (25%) of PEG-8000 concentration. 【Results】 With the increase of PEG-8000 concentration, the germination rate, germination potential, germination index, vigor index, dry weight of vegetative organs, plant length, contents of soluble sugar and peroxidase decreased at seedling stage.While the contents of malondialdehyde and proline significantly increased.Simultaneously, the average of stem diameter, volume and average diameter of root increased first and then decreased at seedling stage.Significant effects were found on cotton seed germination rate and germination potential under different delinting methods.Among them, germination rate, germination potential, root volume, average root diameter, the contents of proline and peroxidase showed T₁ > T₂ > T₃ under three delinting methods.The germination index, vigor index, average stem diameter, root length and root surface area were performed as T₁ > T₃ > T₂.The contents of soluble sugar and malondialdehyde indicated T₃ > T₂ > T₁ among three delinting modes. 【Conclusion】 Compared with delinting method of sulfuric acid depilation, the germination rate and germination potential of cotton seeds are reduced using laser depilation method, the seed vitality and drought resistance is improved.