Abstract: 【Objective】 To determine the optimal solid-state fermentation substrate and culture conditions for the production of volatile antibacterial substances by using the yeast strain 0939-5 as the test material. 【Methods】 The solid nutrients and culture conditions of the strain were optimized by single factor method. 【Results】 The optimum solid-state fermentation substrate of yeast strain 0939-5 was corn meal, the optimum carbon source concentration was glucose 2%, and the optimum inorganic salt concentration was 0.1% MgSO₄.The optimum conditions for solid-state fermentation of yeast strain 0939-5 were inoculation concentration 1×10⁸ CFU/mL, inoculation volume 10%, initial pH 4, and initial water content 40%. 【Conclusion】 On the basis of the basal medium, corn meal was used as a solid fermentation substrate, and 2% glucose and 0.1% MgSO₄ were added respectively, and the optimumculture conditions were inoculum concentration of 1×10⁸ CFU/mL, the inoculum volume of 10%, the initial pH of 4, and the initial water content 40% at 25℃ for 5 days.