Abstract: 【Objective】 To explore the effects of different proportions of organic fertilizers replacing chemical fertilizers on soil nutrient content, plant nutrient uptake, fertilizer utilization rate and sugar beet yield, so as to provide a scientific fertilizer program for achieving high and stable crop yield and soil fertilizer cultivation. 【Methods】 A total of 7 treatments were set up: No fertilization control (CK), household conventional fertilization (CF), humic acid organic fertilizer replacing 10% chemical fertilizer (10%HF), humic acid organic fertilizer replacing 20% chemical fertilizer (20%HF), humic acid organic fertilizer replacing 30% chemical fertilizer (30%HF), humic acid organic fertilizer replacing 40% chemical fertilizer (40%HF), Humic acid organic fertilizer replacing 50% chemical fertilizer (50%HF). Soil nutrients, nutrient uptake by sugar beet plants, dry matter accumulation and sugar beet yield were measured. 【Results】 The results showed that compared with conventional fertilizer treatment, the content of organic fertilizer in soil was increased by 2.37%-7.38% by humic acid fertilizer. Alkali-hydrolyzed nitrogen, available phosphorus and available potassium increased by 1.07%-11.39%, 10.65%-37.83% and 6.04%-14.68%, respectively. 30%HF and 40%HF increased significantly by 16.91% and 17.65% compared with CF treatment, respectively. 【Conclusion】 Compared with conventional application of chemical fertilizer by farmers, organic humic acid fertilizer instead of chemical fertilizer in different proportions can significantly increase soil nutrient content, increase nutrient uptake of beet plants, increase dry matter accumulation of beet, and achieve the purpose of reducing chemical fertilizer, changing soil and increasing yield. Among them, organic fertilizer instead of 30% chemical fertilizer treatment has the better effect of increasing yield and improving soil fertility.