一株海洋杆菌属新菌种XAAS-72T的植物促生功能分析及ACC脱氨酶蛋白结构预测

Structure prediction of the ACC protein from Pontibacter kalidii XAAS-72T with the plant growth-promoting character

    摘要
  • 摘要: 【目的】 研究海洋杆菌属新菌种XAAS-72的植物促生功能,挖掘其潜在功能基因。 【方法】 通过对菌株全基因组测序,分析相关功能基因组成,挖掘ACC脱氨酶合成相关的候选基因,并进行功能预测。 【结果】 海洋杆菌属新种Pontibacter kalidii XAAS-72菌悬液处理可显著提高盆栽小麦麦苗生长,其基因组长度为5 054 860 bp,含1个环形质粒,总GC含量为54.52%,注释的基因数目为4 391个,编码蛋白数4 261个,具有多种抗逆和促生相关基因。其与Pontibacter sp. BAB1700的ACC脱氨酶(1-aminoeyclopropane-1-earboxylate-deaminase)相似度最高为72.48%。该蛋白属于不稳定亲水性蛋白,不具备跨膜结构,且无信号肽结构。 【结论】 海洋杆菌属新种XAAS-72蕴藏着丰富的抗逆和植物促生相关基因。

     

    Abstract: 【Objective】 To explore the plant growth promotion function of a novel strain Pontibacter kalidii XAAS-72T, and investigate the potential functional genes. 【Methods】 The strain whole genome was sequenced, and the composition of function genes were analyzed. A candidate gene related to ACC deaminase synthesis was obtained, and the enzyme protein characteristics were predicted. 【Results】 The results showed that the growth of wheat seed treated with Pontibacter kalidii XAAS-72T was promoted significantly in pots.The genome length of strain XAAS-72 (accession no. CP111079) was 5,054,860 bp, containing one circular plasmid. A total GC content was 54.52%, and the number of annotated genes was 4,391, coding for 4,261 proteins. A variety of resistance and growth-promoting related genes was observed. It had the highest similarity of 72.48% to the ACC deaminase of Pontibacter sp. BAB1700. It an unstable hydrophilic protein, with none of transmembrane structure and signal peptide structure. 【Conclusion】 Pontibacter kalidii XAAS-72T harbors a lot of genes related to the stress resistance and the plant growth-promoting.

     

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