Abstract: 【Objective】 To further investigate the effects of organic inorganic combination application on winter wheat growth and soil quality. 【Methods】 A randomized block design of experiments was used to set six treatments: no fertilization, farmers' conventional fertilization, manure rplacing 25%(CF+M25) chemical fertilizer, manure replacing 50%(CF+M50) chemical fertilizer, manure replacing 75%(CF+M75) chemical fertilizer, and single application of manure. 0-20 cm of arable soil was collected at the turning green, jointing, flowering, and filling stages, and the internal relationship between soil enzyme activity and wheat yield, soil nutrients under the condition of organic and inorganic fertilizer application at each growth stage was discussed. 【Results】 Compared with the conventional fertilization treatment for farmers, 25% and 50% manure treatment increased the number of ears and grains per ear per unit area; The yield of winter wheat under M treatment was 16.2%, 15.9% and 16.8% lower than those under 25%(CF+M25), 50%(CF+M50) and 75%(CF+M75) manure replacement treatments, respectively; The total nitrogen content of CF+M25 and CF+M50 treatments showed a continuous increasing trend, reaching its maximum value during the mature period. The highest available potassium content was observed in the M treatment at each growth stage, which showed a significant difference compared to other treatments. It reached its maximum value of 297.5 mg/kg during the jointing stage, significantly increasing by 50.5% compared to the CF treatment; Soil enzyme activity was highly significantly correlated with available phosphorus content, but not significantly correlated with available potassium. 【Conclusion】 Soil nutrients, enzyme activities and winter wheat yield are closely related. The combination of manure with 30% can achieve the theoretical maximum yield.