Abstract: 【Objective】 To study the effects of microbial agent Junlintianxia on the wheat growth and yield and provide reference for the application of microbial agent in Xinjiang winter wheat. 【Methods】 Under the condition of field drip irrigation, wheat varieties Xindong 22, Xindong 52, Xindong 53 and Shidong 0358 were used to analyze the effects of no microbial agent Junlintianxia and microbial agent Junlintianxia on the growth and yield of winter wheat. 【Results】 The rate of emergence of wheat was slightly higher than that of the control or the same as that, the time of heading to maturity was slightly higher or the same, and the growth period was almost the same. The average plant height, tiller number and stem diameter increased 0.5 cm, 0.4 and 1.1 mm, respectively. The control effect of root rot was the best in No. 103 group (25.5%). The average weight per spike increased by 0.1g, the average number of grains per spike increased by 2.3 grains, the average yield per mu increased by 38.7 kg/667m², and the yield per unit yield of the experiment field increased by 6%, higher than that of the control field. The average net increase was 111.4 yuan/667m² compared with the control field. 【Conclusion】 The application of microbial agent (or Junlintianxia) has no obvious effect on the growth period of wheat, but has seedling preservation effect on winter wheat, which promotes wheat growth and development, has control effect on winter wheat diseases, and increasing effect on winter wheat yield.