Abstract: 【Objective】 Study on impact of reduced nitrogen fertilization combined with bio-organic fertilizer on spring wheat yield enhancement and soil enrichment. 【Methods】 A field enrichment experiment was conducted at Shihuzi Village Farm in Wugongtai Town, Hutubi County, Changji Prefecture, Xinjiang to study the effects of organic fertilizer on soil organic matter and nutrient levels. Six treatments were employed: a control without fertilizer application (CK), a conventional fertilizer application (CF), two levels of nitrogen reduction (T₁D₁, T₁D₂, reduced by 15% and 30%, respectively), and two bio-organic fertilizer application rates (T₂D₁, T₂D₂, at 1,125 kg/hm² and 2,250 kg/hm²). Each treatment was replicated four times. 【Results】 In comparison to CF, treatments combining reduced nitrogen and bio-organic fertilizer application exhibited improved leaf area index (LAI) and soil plant analysis development (SPAD) values for spring wheat. The theoretical yield of spring wheat increased by 9.03% to 28.84%. The nitrogen reduction treatments lowered soil pH and electrical conductivity in the 0-10 cm and 10-20 cm soil layers, while augmenting soil organic matter. With the same nitrogen reduction, the treatment with 2,250 kg/hm² bio-organic fertilizer displayed higher soil nutrient content compared with the treatment with 1,125 kg/hm². When applying the same quantity of bio-organic fertilizer, the 15% nitrogen reduction treatment had greater soil nutrient content than the 30% reduction treatment. In contrast to CK and CF treatments, the treatments integrating reduced nitrogen and bio-organic fertilizer application exhibited increased populations of soil bacteria and actinomycetes, whereas soil fungal populations diminished. 【Conclusion】 A 15% reduction in nitrogen fertilization coupled with bio-organic fertilizer application emerges as an effective fertilization strategy, which encourages prudent utilization of fertilizer resources, enhances soil conditions, promots spring wheat growth in saline-alkali lands, reduces soil pH and electrical conductivity, boosts soil nutrient levels, and optimizes soil microbiota composition.