Abstract: 【Objective】 Study on the differences in seed vitality between different grain sizes of spring wheat varieties and grainsizes. 【Methods】 The purpose of the study is to determine the differ by using 3 kinds of calculation methods on varieties and sizes spring wheat. 【Results】 The results showed that when using different calculation methods for the calculation of different varieties of seed vigor, the results were very significantly different; The ranking of seed vigor size was also inconsistent between different varieties; In addition, when the calculation methods were used to calculate the seed vigor, differences were shown between large seeds and small ones in different varieties. Therefore, the comparison of seed vigor of different spring wheat varieties should not be only based on the calculation method of seed vigor index, but also according to the comprehensive agronomic characters and physiological and biochemical characteristics to make the judgment. 【Conclusion】 Spring wheat seeds of the same variety which have large size and high 1000 grain weight show high vigor. There is no obvious relationship between the seed size and 1000 seed weight of different varieties and the seed vigor, so the seed vigor cannot be determined by a single index. The same varieties, large size or high grain spring wheat seeds, its energy is higher, different varieties, the size of the volume and level of high and low and seed vigor and has no obvious relation, so we can't just rely on a single index to determining the seed vigor