Abstract: 【Objective】 To compare the biological characteristics of defoliating cotton verticillium wilt pathogens from Changji and Shawan and clarify the control effects of carbendazim, ethyl allicin, ethyl azolone, that is, Bacillus subtilis and Trichoderma harzicum, which were commonly used in local production, on cotton verticillium wilt will be determined, and efficient and effective agents selected to guide the control of cotton disease. 【Methods】 The mycelial growth rate method was used to investigate the main biological characteristics of various pathogenic bacteria through adaptive growth of different ions, pH, carbon sources, and temperature. Meanwhile,the toxicity of five pesticides was measured indoors. 【Results】 The optimal temperature for the growth of pathogenic bacteria in Changji City and Shawan City was 20-25 ℃, the optimal ion culture medium was Ca²⁺, the optimal pH was 5-7, and the optimal carbon source was sucrose. All the 5 tested drugs had significantly different inhibitory effects on various pathogenic bacteria. Carbendazim, Bacillus subtilis and Trichoderma harzianum had strong inhibitory effects on pathogenic bacteria. The EC₅₀ of carbendazim, Bacillus subtilis and Trichoderma harzianum to Changji was respectively 0.292,3-0.819,3 μg/mL,1.661×10^-2-2.695×10^-2 μg/mL, 0.101,5-0.884,6 μg/mL, for Shawan, 0.358,8 -5.694 μg/mL, 1.813 ×10^-2-2.727 ×10^-2 μg/mL,0.181,0-0.608,8 μg/mL, respectively, while allicin and azolidinone had the worst control effect on pathogenic bacteria, with an EC₅₀ of 71.04 μg/mL、111.6 μg/mL in the Changji area and in the Shawan area 79.22 and 171.7 μg/mL. 【Conclusion】 In Changji and Shawan, 25 ℃ is the ideal temperature for the growth of several pathogens, and the ideal ion medium is the one that contains Ca²⁺. Different infections develop fastest in pH values between 5 and 7, therefore acidic environments are ideal for their growth. Additionally, they can withstand alkaline conditions well. Microsclerotia develop quickest on culture media containing sucrose as the carbon source and are more likely to occur in alkaline settings. Carbendazim, Bacillus subtilis and Trichoderma harzianum have strong bacteriostatic effects on cotton Vverticillium wilt in Changji and Shawan, which can be used to prevent and control the cotton disease.