Abstract: 【Objective】 To study the effects of different nitrogen inhibitors on the growth, development, nitrogen utilization, and yield of cotton when nitrogen fertilizer is added. The purpose is to determine the appropriate concentration of nitrogen inhibitors. 【Methods】 A pot experiment was conducted using 'China Cotton Research Institute 100' cotton plants. Under the same nitrogen application level, four types of nitrogen inhibitors, 3,4-dimethylpyrazole phosphate (DMPP), 2-chloro-6-trichloromethylpyridine (CP), dicyandiamide (DCD), and N-butylthiophosphoric triamide (NBPT) were added. And two different concentrations were used for each nitrogen inhibitor. The inhibitors were applied as base fertilizer and topdressing during the flowering period, along with urea. Cotton agronomic traits, biomass quality, nitrogen accumulation, lint cotton yield, and nitrogen fertilizer utilization were compared. 【Results】 Compared to the treatment with urea alone, the cotton plants treated with nitrogen inhibitors had a 6.5% to 16.8% increase in individual plant biomass quality during the flowering period, and a 8.8% to 33.0% increase in individual plant lint cotton yield. The apparent nitrogen fertilizer utilization rate increased by 4.5 to 15.1 percentage points. Among the treatments, cotton treated with 0.5% dicyandiamide (DCD), 0.25% 2-chloro-6-trichloromethylpyridine (CP), 1.0% 3,4-dimethylpyrazole phosphate (DMPP), and 0.5% N-butylthiophosphoric triamide (NBPT) added to urea showed better results in terms of biomass accumulation, nitrogen fertilizer utilization, and yield compared to cotton treated with 1.0% DCD, 0.5% CP, 2.0% DMPP, and 1.0% NBPT added to urea. 【Conclusion】 Cotton plants treated with 0.5% DCD added to urea have the highest individual plant biomass accumulation, and cotton treated with 0.25% CP added to urea shows better increases in cotton yield and nitrogen fertilizer utilization compared to other nitrogen inhibitors treatments.