Abstract: 【Objective】 To explore the differences of microbial species and abundance in different types of Aphis gossypii Glove. 【Methods】 The V3-V4 region of 16SrRNA gene of symbiotic bacteria in different types of A. gossypii in cotton fields was sequenced by HiSeq platform, and the species and diversity of symbiotic bacteria in green bug bugs were analyzed. 【Results】 The dominant phylum of A. gossypii in the control group, serratia and Hamiltonella A. gossypii was Proteus, and the relative species abundance accounted for 97.42%, 95.55% and 92.78%, respectively. The dominant bacteria in the control group and the experimental group were Enterobacteriaceae, but the relative abundance was different, accounting for 96.14%, 81.285% and 84.22%, respectively. Compared with the control group, the microbial abundance of Hamiltonella and Serratia increased significantly, accounting for 77.40% and 12.04%, respectively. 【Conclusion】 The microbial abundance of A. gossypii containing Serratia and Hamiltonella in cotton field is significantly affected, and the relative abundance of Hamiltonella and Serratia increased significantly.