Abstract: 【Objective】 To isolate and characterization phytase production probiotic lactic acid bacteria. 【Methods】 Morphologically different strains were isolated from fermented feed, boza and Jiangshui. Through enzyme production screening and 16S rDNA molecular identification, phytase producing lactic acid bacteria strains were obtained and evaluated through acid tolerance, bile salt tolerance, intestinal and gastric juice tolerance, self-aggregation ability, and antibacterial analysis. 【Results】 Four phytase producing lactic acid bacteria were identified. Among them, strain JS5, which was 100% similar to Lactobacillus paracasei JCM 1171, with a maximum phytase activity of 0.83 U/mL. The strain was designated as lactobacillus paracasei. The survival rate of strain JS5 under pH 3 was as high as 100%. At a bile salt concentration of 0.1%, the survival rate of JS5 was 0.55%. In simulated gastric juice and intestinal juice, the survival rates were 8.56% and 0.38%,respectively, and the number of viable bacteria was above 1×10⁶CFU/mL, which was the lowest viable bacteria number with beneficial effects. The self-aggregation ability of strain JS5 was (9.69±0.44), which can promote the colonization of strain JS5 in the intestine. It had inhibitory effects on Escherichia coli, Salmonella, Staphylococcus aureus, Candida albicans, and Pseudomonas aeruginosa. 【Conclusion】 Lactobacillus paracasei JS5 has a high phytase production and can survive in simulated gastrointestinal fluid. It has good cell adhesion ability and inhibits the growth of pathogenic bacteria and can be used as a probiotics for livestock and poultry.