Abstract: 【Objective】 The characters of different germplasm of Isatis indigotica in Xinjiang were studied,the excellent germplasm suitable for local planting was screened out. 【Methods】 32 cultivated germplasm of Isatis indigotica were collected from the whole production area of Isatis indigotica.Grown in manas county,Changii Hui Autonomous Prefecturd Province.The agronomic traits and quality traits were taken as indexes.To analyze the characters of Isatis indigotica germplasm in Xinjiang. 【Results】 The results showed that most of the germplasms had light brown, oblanceolate fruits with length/width ranging from 2.2 to 5.0, thickness ranging from 1 to 3, and thousand-grain weight ranging from 6 to 20 g. The leaf shape was mostly elliptic, the leaf margin shape was mostly medium and weak in strength, the degree of leaf margin notch was mainly light and medium, the anthocyanic colouring was evenly distributed in four degrees (none, weak, medium and strong), and the leaf length was mostly concentrated in 15-25 cm. Root length was concentrated at 35-45 cm, root width at 1.5-2.5 cm, and number of lateral roots at 4-6. The single root weight was concentrated at 100-150 g, with XJ-1 and XJ-2 being the outstanding germplasms. There were significant differences in (R, S)-goitrin content among the germplasms, with XJ-1 and HN-1 germplasms being the excellent performers. There was a highly significant negative correlation between the number of lateral roots and root length, and a highly significant positive correlation with root width. There was a highly significant positive correlation between root weight and root width and number of lateral roots. Root width was negatively correlated with root length. 【Conclusion】 There are differences in the performance of traits among the various germplasm in Xinjiang, with HLJ-1 showing excellent performance in agronomic traits such as root length and leaf length, and XJ-1 showing relative advantages in both root weight and active ingredient content.