谷子萌发期适宜盐胁迫浓度筛选及耐盐性评价

Screening of suitable salt concentration and evaluation of salt tolerance during the germination period of foxtail millet

  • 摘要:
    目的 筛选谷子萌发期适宜NaCl盐溶液胁迫浓度,建立谷子萌发期耐盐性评价方法。
    方法 用100、150、200、250 mmol/L 4个浓度的NaCl盐溶液对10份不同来源谷子种质进行胁迫,以发芽势、发芽率、发芽指数、活力指数、根长、芽长和鲜重为评价指标,筛选出谷子萌发期耐盐性评价的NaCl盐溶液浓度,进一步应用隶属函数法对筛选出的NaCl盐溶液浓度下各谷子种质萌发期的耐盐性进行综合评价。
    结果 在150 mmol/L NaCl盐溶液胁迫下,40%的谷子种质发芽率与对照相比,存在显著或者极显著差异;80%以上的谷子种质的发芽势、发芽指数、活力指数、胚根长度、胚芽长度、鲜重与对照相比,存在显著或者极显著差异。陇谷13的D值为0.922,天粟G12的D值为0.270。
    结论 150 mmol/L的NaCl盐溶液可以用于谷子萌发期的耐盐性评价;10份谷子种质中,耐盐性最强的是陇谷13,最差的是天粟G12。

     

    Abstract:
    Objective Screening suitable NaCl salt solution concentrations for salt tolerance evaluation during the germination stage of foxtail millet, and to establish a method for salt tolerance evaluation during the germination stage of foxtail millet.
    Methods Ten different varieties of foxtail millet were subjected to salt stress using NaCl solutions at four concentrations (100, 150, 200, and 250 mmol/L). Germination potential, germination rate, germination index, vitality index, radicle length, plumule length, and fresh weight were used as evaluation indicators to screen the NaCl salt solution concentration for salt tolerance evaluation during foxtail millet germination. The membership function method was further applied to comprehensively evaluate the salt tolerance of each variety under the selected NaCl solution concentration.
    Results Under the salt stress of a 150 mmol/L NaCl salt solution, 40% of the varieties showed significant or extremely significant differences in germination rate compared to the control; more than 80% of varieties show significant or extremely significant differences in germination potential, germination index, vitality index, radicle length, plumule length, and fresh weight compared to the control. The D value of Longgu 13 is 0.922, and the D value of Tiansu G12 is 0.270.
    Conclusion 150 mmol/L NaCl salt solution can be used for evaluating salt tolerance during the germination stage of foxtail millet; among the 10 varieties of foxtail millet, Longgu 13 has the strongest salt tolerance, while Tiansu G12 has the worst.

     

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